Jens Randel Nyengaard

Tissue shrinkage and unbiased stereological estimation of particle number and size.

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Tissue shrinkage and unbiased stereological estimation of particle number and size. / Dorph-Petersen, K A; Nyengaard, J R; Gundersen, H J.

In: Journal of Microscopy, Vol. 204, No. Pt 3, 2001, p. 232-46.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Author

Dorph-Petersen, K A ; Nyengaard, J R ; Gundersen, H J. / Tissue shrinkage and unbiased stereological estimation of particle number and size. In: Journal of Microscopy. 2001 ; Vol. 204, No. Pt 3. pp. 232-46.

Bibtex

@article{87c443608bb911ddbd84000ea68e967b,
title = "Tissue shrinkage and unbiased stereological estimation of particle number and size.",
abstract = "This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paper introduces new estimators to be used in optical fractionator and optical disector designs; the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.",
keywords = "Brain, Humans, Male, Microscopy, Microtomy, Particle Size, Tissue Embedding, Tissue Fixation",
author = "Dorph-Petersen, {K A} and Nyengaard, {J R} and Gundersen, {H J}",
year = "2001",
doi = "10.1046/j.1365-2818.2001.00958.x",
language = "English",
volume = "204",
pages = "232--46",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "Pt 3",

}

RIS

TY - JOUR

T1 - Tissue shrinkage and unbiased stereological estimation of particle number and size.

AU - Dorph-Petersen, K A

AU - Nyengaard, J R

AU - Gundersen, H J

PY - 2001

Y1 - 2001

N2 - This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paper introduces new estimators to be used in optical fractionator and optical disector designs; the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.

AB - This paper is a review of the stereological problems related to the unbiased estimation of particle number and size when tissue deformation is present. The deformation may occur during the histological processing of the tissue. It is especially noted that the widely used optical disector may be biased by dimensional changes in the z-axis, i.e. the direction perpendicular to the section plane. This is often the case when frozen sections or vibratome sections are used for the stereological measurements. The present paper introduces new estimators to be used in optical fractionator and optical disector designs; the first is, as usual, the simplest and most robust. Finally, it is stated that when tissue deformation only occurs in the z-direction, unbiased estimation of particle size with several estimators is possible.

KW - Brain

KW - Humans

KW - Male

KW - Microscopy

KW - Microtomy

KW - Particle Size

KW - Tissue Embedding

KW - Tissue Fixation

U2 - 10.1046/j.1365-2818.2001.00958.x

DO - 10.1046/j.1365-2818.2001.00958.x

M3 - Journal article

C2 - 11903800

VL - 204

SP - 232

EP - 246

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - Pt 3

ER -