Jens Randel Nyengaard

Stereological estimation of particle shape from vertical sections

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Stereological estimation of particle shape from vertical sections. / Larsen, Nick Y.; Ziegel, Johanna F.; Nyengaard, Jens R.; Jensen, Eva B. Vedel.

In: Journal of Microscopy, Vol. 275, No. 3, 09.2019, p. 183-194.

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Author

Larsen, Nick Y. ; Ziegel, Johanna F. ; Nyengaard, Jens R. ; Jensen, Eva B. Vedel. / Stereological estimation of particle shape from vertical sections. In: Journal of Microscopy. 2019 ; Vol. 275, No. 3. pp. 183-194.

Bibtex

@article{55966971ff38485e8a21e64b294e85a5,
title = "Stereological estimation of particle shape from vertical sections",
abstract = "In the present paper, we describe a new simple stereological method of estimating volume tensors in 3D from vertical sections. The volume tensors provide information about particle shape in 3D. In a model-based setting, the method requires that the particle distribution is invariant under rotations around the vertical axis. In a design-based approach, where the vertical section is uniformly rotated around the vertical axis, the method provides information about an index of elongation of the particles in the direction of the vertical axis. The method has been implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus of Brodmann Area 46. In the actual implementation, the new estimator shows similar precision as an earlier estimator, based on an optical rotator design, but it is a factor 3 faster to collect the measurements for the new estimator. Furthermore, the calculations needed for determining the new estimator are much simpler. Lay Description A new method is described for estimating volume tensors in 3 dimensions based on the stereological method: planar rotator. In general, volume tensors may provide information about particle volume, shape and direction. The new estimator was implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus on Brodmann Area 46 and compared to a previous published method, based on an optical rotator design. The new estimator shows similar precision as the earlier estimator, but it is a factor 3 faster to collect the measurements. Besides, the calculations behind the new estimator are simpler and easier to implement to a software program.",
keywords = "Particle processes, rotational invariance, shape, stereology, vertical sections, volume tensors, NORMAL HUMAN CORTEX, CYTOARCHITECTONIC DEFINITION, PREFRONTAL AREAS, ORIENTATION, FIBROBLASTS, DENSITY, NEURONS",
author = "Larsen, {Nick Y.} and Ziegel, {Johanna F.} and Nyengaard, {Jens R.} and Jensen, {Eva B. Vedel}",
year = "2019",
month = sep,
doi = "10.1111/jmi.12826",
language = "English",
volume = "275",
pages = "183--194",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "3",

}

RIS

TY - JOUR

T1 - Stereological estimation of particle shape from vertical sections

AU - Larsen, Nick Y.

AU - Ziegel, Johanna F.

AU - Nyengaard, Jens R.

AU - Jensen, Eva B. Vedel

PY - 2019/9

Y1 - 2019/9

N2 - In the present paper, we describe a new simple stereological method of estimating volume tensors in 3D from vertical sections. The volume tensors provide information about particle shape in 3D. In a model-based setting, the method requires that the particle distribution is invariant under rotations around the vertical axis. In a design-based approach, where the vertical section is uniformly rotated around the vertical axis, the method provides information about an index of elongation of the particles in the direction of the vertical axis. The method has been implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus of Brodmann Area 46. In the actual implementation, the new estimator shows similar precision as an earlier estimator, based on an optical rotator design, but it is a factor 3 faster to collect the measurements for the new estimator. Furthermore, the calculations needed for determining the new estimator are much simpler. Lay Description A new method is described for estimating volume tensors in 3 dimensions based on the stereological method: planar rotator. In general, volume tensors may provide information about particle volume, shape and direction. The new estimator was implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus on Brodmann Area 46 and compared to a previous published method, based on an optical rotator design. The new estimator shows similar precision as the earlier estimator, but it is a factor 3 faster to collect the measurements. Besides, the calculations behind the new estimator are simpler and easier to implement to a software program.

AB - In the present paper, we describe a new simple stereological method of estimating volume tensors in 3D from vertical sections. The volume tensors provide information about particle shape in 3D. In a model-based setting, the method requires that the particle distribution is invariant under rotations around the vertical axis. In a design-based approach, where the vertical section is uniformly rotated around the vertical axis, the method provides information about an index of elongation of the particles in the direction of the vertical axis. The method has been implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus of Brodmann Area 46. In the actual implementation, the new estimator shows similar precision as an earlier estimator, based on an optical rotator design, but it is a factor 3 faster to collect the measurements for the new estimator. Furthermore, the calculations needed for determining the new estimator are much simpler. Lay Description A new method is described for estimating volume tensors in 3 dimensions based on the stereological method: planar rotator. In general, volume tensors may provide information about particle volume, shape and direction. The new estimator was implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus on Brodmann Area 46 and compared to a previous published method, based on an optical rotator design. The new estimator shows similar precision as the earlier estimator, but it is a factor 3 faster to collect the measurements. Besides, the calculations behind the new estimator are simpler and easier to implement to a software program.

KW - Particle processes

KW - rotational invariance

KW - shape

KW - stereology

KW - vertical sections

KW - volume tensors

KW - NORMAL HUMAN CORTEX

KW - CYTOARCHITECTONIC DEFINITION

KW - PREFRONTAL AREAS

KW - ORIENTATION

KW - FIBROBLASTS

KW - DENSITY

KW - NEURONS

U2 - 10.1111/jmi.12826

DO - 10.1111/jmi.12826

M3 - Journal article

C2 - 31328794

VL - 275

SP - 183

EP - 194

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - 3

ER -