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Jens Randel Nyengaard

Stereological estimation of particle shape and orientation from volume tensors

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DOI

In the present paper, we describe new robust methods of estimating cell shape and orientation in 3D from sections. The descriptors of 3D cell shape and orientation are based on volume tensors which are used to construct an ellipsoid, the Miles ellipsoid, approximating the average cell shape and orientation in 3D. The estimators of volume tensors are based on observations in several optical planes through sampled cells. This type of geometric sampling design is known as the optical rotator. The statistical behaviour of the estimator of the Miles ellipsoid is studied under a flexible model for 3D cell shape and orientation. In a simulation study, the lengths of the axes of the Miles ellipsoid can be estimated with coefficients of variation of about 2% if 100 cells are sampled. Finally, we illustrate the use of the developed methods in an example, involving neurons in the medial prefrontal cortex of rat.

Original languageEnglish
JournalJournal of Microscopy
Volume263
Issue3
Pages (from-to)229-237
Number of pages9
ISSN0022-2720
DOIs
Publication statusPublished - 29 Jan 2016

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