Jens Randel Nyengaard

Kidney biopsies can be used for estimations of glomerular number and volume: a pig study.

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  • Institute of Clinical Medicine
  • Center of Magnetic Resonance
  • Stereological Research Laboratory
In this study, we wanted to evaluate the use of kidney biopsies for estimation of N(glom) and V(glom) in both healthy and chronically diseased kidneys. Danish Landrace pigs with mean weight of 29 kg (range: 25-35 kg) were either subjected to unilateral ureteral obstruction (UUO) or non-obstruction (healthy). N(glom) and V(glom) was estimated by design-based methods using biopsies, N(glom)(biopsy) and V(glom)(biopsy). From each kidney, six biopsies were withdrawn at six topographically different sites. All estimates were done following stereological principles and reference methods estimated number with the physical fractionator, N(glom)(PF), and volume with test point system, V(glom)(TPS). N(glom)(PF) was 1.30 +/- 0.28 x 10(6) for UUO kidneys and 1.59 +/- 0.28 x 10(6) for healthy kidneys. N(glom)(biopsy) was 1.18 +/- 0.28 x 10(6) (p > 0.05) for UUO and 1.33 +/- 0.24 x 10(6) (p = 0.04) for healthy kidneys. When UUO and healthy kidneys were grouped, N(glom)(PF) was 1.46 +/- 0.31 x 10(6), and N(glom)(biopsy) was 1.28 +/- 0.25 x 10(6) (p > 0.05). V(glom)(TPS) was 1,079 +/- 126 mm(3) for UUO and 1,707 +/- 263 mm(3) for healthy kidneys. V(glom)(biopsy) was 1,048 +/- 291 mm(3) for UUO (p > 0.05) and 1,373 +/- 393 mm(3) for healthy kidneys (p > 0.05). When UUO and healthy kidneys were grouped, V(glom)(TPS) was 1,180 +/- 229 mm(3) and V(glom)(biopsy) 1,129 +/- 334 mm(3) (p > 0.05). Biopsy sites were tested for any systematic differences between site- and mean values, and no significant difference was found (p > 0.05). This study showed that biopsies can be used for estimating N(glom) and V(glom) by design-based methods, but more precise determination of biopsy volume is needed.
Original languageEnglish
JournalVirchows Archiv
Volume452
Issue4
Pages (from-to)393-403
Number of pages10
ISSN0945-6317
DOIs
Publication statusPublished - 2008

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