Jens Randel Nyengaard

IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner. / Botting, Kimberley Jae; Loke, Xin Yee; Zhang, Song; Andersen, Johnnie B; Nyengaard, Jens Randel; Morrison, Janna L.

In: A J P: Regulatory, Integrative and Comparative Physiology (Online), Vol. 315, No. 1, 07.2018, p. 48-67.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Botting, KJ, Loke, XY, Zhang, S, Andersen, JB, Nyengaard, JR & Morrison, JL 2018, 'IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner', A J P: Regulatory, Integrative and Comparative Physiology (Online), vol. 315, no. 1, pp. 48-67. https://doi.org/10.1152/ajpregu.00180.2017

APA

Botting, K. J., Loke, X. Y., Zhang, S., Andersen, J. B., Nyengaard, J. R., & Morrison, J. L. (2018). IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner. A J P: Regulatory, Integrative and Comparative Physiology (Online), 315(1), 48-67. https://doi.org/10.1152/ajpregu.00180.2017

CBE

Botting KJ, Loke XY, Zhang S, Andersen JB, Nyengaard JR, Morrison JL. 2018. IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner. A J P: Regulatory, Integrative and Comparative Physiology (Online). 315(1):48-67. https://doi.org/10.1152/ajpregu.00180.2017

MLA

Botting, Kimberley Jae et al. "IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner". A J P: Regulatory, Integrative and Comparative Physiology (Online). 2018, 315(1). 48-67. https://doi.org/10.1152/ajpregu.00180.2017

Vancouver

Botting KJ, Loke XY, Zhang S, Andersen JB, Nyengaard JR, Morrison JL. IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner. A J P: Regulatory, Integrative and Comparative Physiology (Online). 2018 Jul;315(1):48-67. https://doi.org/10.1152/ajpregu.00180.2017

Author

Botting, Kimberley Jae ; Loke, Xin Yee ; Zhang, Song ; Andersen, Johnnie B ; Nyengaard, Jens Randel ; Morrison, Janna L. / IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner. In: A J P: Regulatory, Integrative and Comparative Physiology (Online). 2018 ; Vol. 315, No. 1. pp. 48-67.

Bibtex

@article{bead64479cf746a1b4651886b89fbef5,
title = "IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner",
abstract = "Intrauterine growth restriction (IUGR) increases the risk of ischaemic heart disease in adulthood. Studies in rats suggest cardiac vulnerability is more pronounced in males and in offspring that were exposed to hypoxia in utero. Therefore, we aimed to test the hypotheses that 1) IUGR adolescent males, but not females, have fewer cardiomyocytes and altered expression of cardiometabolic genes compared to Controls and 2) IUGR due to hypoxia has a greater effect on these parameters compared to IUGR due to nutrient restriction. IUGR was induced in guinea pigs by Maternal Hypoxia (MH, 10% O2, n=9), or Maternal Nutrient Restriction (MNR, ~30% reduction in food intake, n=9) in the second half of pregnancy and compared to Control (n=11). At 120 days of age, post-mortem was performed, and the left ventricle perfusion fixed for stereological determination of cardiomyocyte number or snap frozen to determine the abundance of cardiometabolic genes and proteins by qRT-PCR and Western Blotting, respectively. MH reduced the number of cardiomyocytes in female (P<0.05), but not male or MNR, adolescent offspring. Furthermore, IUGR males had decreased expression of genes responsible for fatty acid activation in the sarcoplasm (FACS) and transport into the mitochondria (AMPKa2 and ACC; P<0.05) and females exposed to MH had increased activation/phosphorylation of AMPK (P<0.05). We postulate that the changes in cardiomyocyte endowment and cardiac gene expression observed in the present study are a direct result of in utero programming as offspring at this age did not suffer from obesity, hypertension or left ventricular hypertrophy.",
keywords = "Cardiac metabolism, Cardiomyocyte development, Intrauterine growth restriction",
author = "Botting, {Kimberley Jae} and Loke, {Xin Yee} and Song Zhang and Andersen, {Johnnie B} and Nyengaard, {Jens Randel} and Morrison, {Janna L}",
year = "2018",
month = jul,
doi = "10.1152/ajpregu.00180.2017",
language = "English",
volume = "315",
pages = "48--67",
journal = "A J P: Regulatory, Integrative and Comparative Physiology (Online)",
issn = "1522-1490",
publisher = "AMER PHYSIOLOGICAL SOC",
number = "1",

}

RIS

TY - JOUR

T1 - IUGR decreases cardiomyocyte endowment and alters cardiac metabolism in a sex and cause of IUGR specific manner

AU - Botting, Kimberley Jae

AU - Loke, Xin Yee

AU - Zhang, Song

AU - Andersen, Johnnie B

AU - Nyengaard, Jens Randel

AU - Morrison, Janna L

PY - 2018/7

Y1 - 2018/7

N2 - Intrauterine growth restriction (IUGR) increases the risk of ischaemic heart disease in adulthood. Studies in rats suggest cardiac vulnerability is more pronounced in males and in offspring that were exposed to hypoxia in utero. Therefore, we aimed to test the hypotheses that 1) IUGR adolescent males, but not females, have fewer cardiomyocytes and altered expression of cardiometabolic genes compared to Controls and 2) IUGR due to hypoxia has a greater effect on these parameters compared to IUGR due to nutrient restriction. IUGR was induced in guinea pigs by Maternal Hypoxia (MH, 10% O2, n=9), or Maternal Nutrient Restriction (MNR, ~30% reduction in food intake, n=9) in the second half of pregnancy and compared to Control (n=11). At 120 days of age, post-mortem was performed, and the left ventricle perfusion fixed for stereological determination of cardiomyocyte number or snap frozen to determine the abundance of cardiometabolic genes and proteins by qRT-PCR and Western Blotting, respectively. MH reduced the number of cardiomyocytes in female (P<0.05), but not male or MNR, adolescent offspring. Furthermore, IUGR males had decreased expression of genes responsible for fatty acid activation in the sarcoplasm (FACS) and transport into the mitochondria (AMPKa2 and ACC; P<0.05) and females exposed to MH had increased activation/phosphorylation of AMPK (P<0.05). We postulate that the changes in cardiomyocyte endowment and cardiac gene expression observed in the present study are a direct result of in utero programming as offspring at this age did not suffer from obesity, hypertension or left ventricular hypertrophy.

AB - Intrauterine growth restriction (IUGR) increases the risk of ischaemic heart disease in adulthood. Studies in rats suggest cardiac vulnerability is more pronounced in males and in offspring that were exposed to hypoxia in utero. Therefore, we aimed to test the hypotheses that 1) IUGR adolescent males, but not females, have fewer cardiomyocytes and altered expression of cardiometabolic genes compared to Controls and 2) IUGR due to hypoxia has a greater effect on these parameters compared to IUGR due to nutrient restriction. IUGR was induced in guinea pigs by Maternal Hypoxia (MH, 10% O2, n=9), or Maternal Nutrient Restriction (MNR, ~30% reduction in food intake, n=9) in the second half of pregnancy and compared to Control (n=11). At 120 days of age, post-mortem was performed, and the left ventricle perfusion fixed for stereological determination of cardiomyocyte number or snap frozen to determine the abundance of cardiometabolic genes and proteins by qRT-PCR and Western Blotting, respectively. MH reduced the number of cardiomyocytes in female (P<0.05), but not male or MNR, adolescent offspring. Furthermore, IUGR males had decreased expression of genes responsible for fatty acid activation in the sarcoplasm (FACS) and transport into the mitochondria (AMPKa2 and ACC; P<0.05) and females exposed to MH had increased activation/phosphorylation of AMPK (P<0.05). We postulate that the changes in cardiomyocyte endowment and cardiac gene expression observed in the present study are a direct result of in utero programming as offspring at this age did not suffer from obesity, hypertension or left ventricular hypertrophy.

KW - Cardiac metabolism

KW - Cardiomyocyte development

KW - Intrauterine growth restriction

U2 - 10.1152/ajpregu.00180.2017

DO - 10.1152/ajpregu.00180.2017

M3 - Journal article

C2 - 29561647

VL - 315

SP - 48

EP - 67

JO - A J P: Regulatory, Integrative and Comparative Physiology (Online)

JF - A J P: Regulatory, Integrative and Comparative Physiology (Online)

SN - 1522-1490

IS - 1

ER -