Jens Randel Nyengaard

Estimating nephron number in the developing kidney using the physical disector/fractionator combination

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearch

  • Luise A. Cullen-Mcewen, Monash University
  • ,
  • James A. Armitage, Monash University
  • ,
  • Jens R. Nyengaard
  • John F. Bertram, Monash University

Design-based stereology is considered the gold-standard method for estimating the total number of glomeruli, and thereby nephrons, in the adult kidney. However, until recently, a design-based method for estimating nephron number in the developing kidney was not available. For such a method to provide accurate and precise estimates, unambiguous identification of developing nephrons is essential. Here, we describe a combined histochemical/stereological technique for estimating total nephron number in the developing mouse and rat kidney. The method can be modified for use in other species.

Original languageEnglish
JournalMethods in Molecular Biology
Pages (from-to)109-119
Number of pages11
Publication statusPublished - 1 Jan 2012

    Research areas

  • Disector, Kidney development, Metanephros, Nephron endowment, Nephron number, Stereology

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