Aarhus University Seal / Aarhus Universitets segl

Jens Randel Nyengaard

Epidermal nerve fiber length density estimation using global spatial sampling in healthy subjects and neuropathy patients

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Epidermal nerve fiber length density estimation using global spatial sampling in healthy subjects and neuropathy patients. / Karlsson, Páll; Møller, Anette Torvin; Jensen, Troels Staehelin; Nyengaard, Jens Randel.

In: Journal of Neuropathology and Experimental Neurology, Vol. 72, No. 3, 03.2013, p. 186-93.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{beaf4022157a4998b60896d252ec2cef,
title = "Epidermal nerve fiber length density estimation using global spatial sampling in healthy subjects and neuropathy patients",
abstract = "Assessment of intraepidermal nerve fiber density (IENFD) has become a useful tool for the investigation of patients with suspected small-fiber neuropathy (SFN). Here, we estimate epidermal nerve fiber lengths in 12 patients with SFN and 36 healthy controls using global spatial sampling and compare the lengths with IENFD and axonal swelling ratios. Skin biopsies were analyzed on 50-μm-thick free-floating sections immunostained for the neuronal cytoplasmic marker PGP 9.5. Mean IENFD in SFN patients was 2.22 ± 1.63 mm versus 7.51 ± 2.17 mm in controls; mean length density was 112 ± 82.6 mm in SFN patients versus 565 ± 240 mm in controls (p < 0.001 for both). The correlation between the nerve fiber length and the IENFD was r = 0.16 for healthy subjects and r = 0.39 for patients, suggesting that these variables provide different quantitative information. There were significant differences in axonal swelling ratios between healthy subjects and patients, that is, per IENFD and per nerve fiber length. Together, these results suggest that, although length estimation requires more time and additional equipment, it is as effective as IENFD in differentiating SFN patients from healthy subjects. Estimating nerve fiber length may increase mechanistic understanding beyond IENFD estimation and improve efficiency in diagnosing SFN.",
keywords = "Adult, Aged, Aged, 80 and over, Biopsy, Epidermis, Female, Humans, Immunohistochemistry, Male, Middle Aged, Nerve Fibers, Peripheral Nervous System Diseases",
author = "P{\'a}ll Karlsson and M{\o}ller, {Anette Torvin} and Jensen, {Troels Staehelin} and Nyengaard, {Jens Randel}",
year = "2013",
month = mar,
doi = "10.1097/NEN.0b013e318284e849",
language = "English",
volume = "72",
pages = "186--93",
journal = "Journal of Neuropathology and Experimental Neurology",
issn = "0022-3069",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Epidermal nerve fiber length density estimation using global spatial sampling in healthy subjects and neuropathy patients

AU - Karlsson, Páll

AU - Møller, Anette Torvin

AU - Jensen, Troels Staehelin

AU - Nyengaard, Jens Randel

PY - 2013/3

Y1 - 2013/3

N2 - Assessment of intraepidermal nerve fiber density (IENFD) has become a useful tool for the investigation of patients with suspected small-fiber neuropathy (SFN). Here, we estimate epidermal nerve fiber lengths in 12 patients with SFN and 36 healthy controls using global spatial sampling and compare the lengths with IENFD and axonal swelling ratios. Skin biopsies were analyzed on 50-μm-thick free-floating sections immunostained for the neuronal cytoplasmic marker PGP 9.5. Mean IENFD in SFN patients was 2.22 ± 1.63 mm versus 7.51 ± 2.17 mm in controls; mean length density was 112 ± 82.6 mm in SFN patients versus 565 ± 240 mm in controls (p < 0.001 for both). The correlation between the nerve fiber length and the IENFD was r = 0.16 for healthy subjects and r = 0.39 for patients, suggesting that these variables provide different quantitative information. There were significant differences in axonal swelling ratios between healthy subjects and patients, that is, per IENFD and per nerve fiber length. Together, these results suggest that, although length estimation requires more time and additional equipment, it is as effective as IENFD in differentiating SFN patients from healthy subjects. Estimating nerve fiber length may increase mechanistic understanding beyond IENFD estimation and improve efficiency in diagnosing SFN.

AB - Assessment of intraepidermal nerve fiber density (IENFD) has become a useful tool for the investigation of patients with suspected small-fiber neuropathy (SFN). Here, we estimate epidermal nerve fiber lengths in 12 patients with SFN and 36 healthy controls using global spatial sampling and compare the lengths with IENFD and axonal swelling ratios. Skin biopsies were analyzed on 50-μm-thick free-floating sections immunostained for the neuronal cytoplasmic marker PGP 9.5. Mean IENFD in SFN patients was 2.22 ± 1.63 mm versus 7.51 ± 2.17 mm in controls; mean length density was 112 ± 82.6 mm in SFN patients versus 565 ± 240 mm in controls (p < 0.001 for both). The correlation between the nerve fiber length and the IENFD was r = 0.16 for healthy subjects and r = 0.39 for patients, suggesting that these variables provide different quantitative information. There were significant differences in axonal swelling ratios between healthy subjects and patients, that is, per IENFD and per nerve fiber length. Together, these results suggest that, although length estimation requires more time and additional equipment, it is as effective as IENFD in differentiating SFN patients from healthy subjects. Estimating nerve fiber length may increase mechanistic understanding beyond IENFD estimation and improve efficiency in diagnosing SFN.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Biopsy

KW - Epidermis

KW - Female

KW - Humans

KW - Immunohistochemistry

KW - Male

KW - Middle Aged

KW - Nerve Fibers

KW - Peripheral Nervous System Diseases

U2 - 10.1097/NEN.0b013e318284e849

DO - 10.1097/NEN.0b013e318284e849

M3 - Journal article

C2 - 23399897

VL - 72

SP - 186

EP - 193

JO - Journal of Neuropathology and Experimental Neurology

JF - Journal of Neuropathology and Experimental Neurology

SN - 0022-3069

IS - 3

ER -