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Jens Randel Nyengaard

Effect of physicochemical properties on intranasal nanoparticle transit into murine olfactory epithelium

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Effect of physicochemical properties on intranasal nanoparticle transit into murine olfactory epithelium. / Mistry, Alpesh; Glud, Sys Zoffmann; Kjems, Jørgen; Nyengaard, Jens Randel; Howard, Kenneth Alan; Stolnik, Snjezana; Illum, Lisbeth.

In: Journal of Drug Evaluation, Vol. 17, No. 7, 2009, p. 543-552.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{027eb9468f044d8182254753c060129f,
title = "Effect of physicochemical properties on intranasal nanoparticle transit into murine olfactory epithelium",
abstract = "Small molecular weight drugs, peptides, and nanoparticles have previously been shown to localize in the central nervous system after intraneural administration. A basic understanding of direct nose-to-brain drug delivery, particularly for nanoparticles with different physicochemical characteristics, remains unclear. In this study, fluorescence microscopy and stereology were used to track intranasally administered chitosan-coated polystyrene (C-PS) or polysorbate-coated polystyrene (P80-PS) nanoparticles (100 nm or 200 nm in diameter) in olfactory and respiratory nasal epithelia and olfactory bulbs in mice. Chitosan coating caused particles to adhere to the extracellular mucus which could provide useful modality for paracellular drug transport. Nanoparticle transport was exclusively transcellular. None of the nanoparticle formulations showed preference for uptake into olfactory axons over other nasal epithelial cells. Both 100 nm PS and 100 nm P80-PS were observed in olfactory epithelial cells but were absent from the olfactory bulbs; therefore, it is speculated that an optimal nanoparticle diameter for axonal transport is ",
keywords = "Adhesiveness, Administration, Intranasal, Animals, Axons, Biological Transport, Chitosan, Drug Delivery Systems, Male, Mice, Mice, Inbred Strains, Microscopy, Fluorescence, Nanoparticles, Olfactory Mucosa, Particle Size, Polysorbates, Polystyrenes, Tissue Distribution",
author = "Alpesh Mistry and Glud, {Sys Zoffmann} and J{\o}rgen Kjems and Nyengaard, {Jens Randel} and Howard, {Kenneth Alan} and Snjezana Stolnik and Lisbeth Illum",
year = "2009",
doi = "10.1080/10611860903055470",
language = "English",
volume = "17",
pages = "543--552",
journal = "The Journal of Drug Evaluation",
issn = "1479-1137",
publisher = "Taylor & Francis ",
number = "7",

}

RIS

TY - JOUR

T1 - Effect of physicochemical properties on intranasal nanoparticle transit into murine olfactory epithelium

AU - Mistry, Alpesh

AU - Glud, Sys Zoffmann

AU - Kjems, Jørgen

AU - Nyengaard, Jens Randel

AU - Howard, Kenneth Alan

AU - Stolnik, Snjezana

AU - Illum, Lisbeth

PY - 2009

Y1 - 2009

N2 - Small molecular weight drugs, peptides, and nanoparticles have previously been shown to localize in the central nervous system after intraneural administration. A basic understanding of direct nose-to-brain drug delivery, particularly for nanoparticles with different physicochemical characteristics, remains unclear. In this study, fluorescence microscopy and stereology were used to track intranasally administered chitosan-coated polystyrene (C-PS) or polysorbate-coated polystyrene (P80-PS) nanoparticles (100 nm or 200 nm in diameter) in olfactory and respiratory nasal epithelia and olfactory bulbs in mice. Chitosan coating caused particles to adhere to the extracellular mucus which could provide useful modality for paracellular drug transport. Nanoparticle transport was exclusively transcellular. None of the nanoparticle formulations showed preference for uptake into olfactory axons over other nasal epithelial cells. Both 100 nm PS and 100 nm P80-PS were observed in olfactory epithelial cells but were absent from the olfactory bulbs; therefore, it is speculated that an optimal nanoparticle diameter for axonal transport is

AB - Small molecular weight drugs, peptides, and nanoparticles have previously been shown to localize in the central nervous system after intraneural administration. A basic understanding of direct nose-to-brain drug delivery, particularly for nanoparticles with different physicochemical characteristics, remains unclear. In this study, fluorescence microscopy and stereology were used to track intranasally administered chitosan-coated polystyrene (C-PS) or polysorbate-coated polystyrene (P80-PS) nanoparticles (100 nm or 200 nm in diameter) in olfactory and respiratory nasal epithelia and olfactory bulbs in mice. Chitosan coating caused particles to adhere to the extracellular mucus which could provide useful modality for paracellular drug transport. Nanoparticle transport was exclusively transcellular. None of the nanoparticle formulations showed preference for uptake into olfactory axons over other nasal epithelial cells. Both 100 nm PS and 100 nm P80-PS were observed in olfactory epithelial cells but were absent from the olfactory bulbs; therefore, it is speculated that an optimal nanoparticle diameter for axonal transport is

KW - Adhesiveness

KW - Administration, Intranasal

KW - Animals

KW - Axons

KW - Biological Transport

KW - Chitosan

KW - Drug Delivery Systems

KW - Male

KW - Mice

KW - Mice, Inbred Strains

KW - Microscopy, Fluorescence

KW - Nanoparticles

KW - Olfactory Mucosa

KW - Particle Size

KW - Polysorbates

KW - Polystyrenes

KW - Tissue Distribution

U2 - 10.1080/10611860903055470

DO - 10.1080/10611860903055470

M3 - Journal article

C2 - 19530905

VL - 17

SP - 543

EP - 552

JO - The Journal of Drug Evaluation

JF - The Journal of Drug Evaluation

SN - 1479-1137

IS - 7

ER -