Jens Randel Nyengaard

Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians. / Harrath, Abdel Halim; Semlali, Abdelhabib; Mansour, Lamjed; Aldahmash, Waleed; Al Omar, Suliman Y.; Al Anazi, Mohamed S.; Nyengaard, Jens R.; Alwasel, Saleh.

In: Biological Bulletin, Vol. 232, No. 1, 02.2017, p. 12-18.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Harrath, AH, Semlali, A, Mansour, L, Aldahmash, W, Al Omar, SY, Al Anazi, MS, Nyengaard, JR & Alwasel, S 2017, 'Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians', Biological Bulletin, vol. 232, no. 1, pp. 12-18. https://doi.org/10.1086/691408

APA

Harrath, A. H., Semlali, A., Mansour, L., Aldahmash, W., Al Omar, S. Y., Al Anazi, M. S., Nyengaard, J. R., & Alwasel, S. (2017). Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians. Biological Bulletin, 232(1), 12-18. https://doi.org/10.1086/691408

CBE

Harrath AH, Semlali A, Mansour L, Aldahmash W, Al Omar SY, Al Anazi MS, Nyengaard JR, Alwasel S. 2017. Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians. Biological Bulletin. 232(1):12-18. https://doi.org/10.1086/691408

MLA

Vancouver

Harrath AH, Semlali A, Mansour L, Aldahmash W, Al Omar SY, Al Anazi MS et al. Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians. Biological Bulletin. 2017 Feb;232(1):12-18. https://doi.org/10.1086/691408

Author

Harrath, Abdel Halim ; Semlali, Abdelhabib ; Mansour, Lamjed ; Aldahmash, Waleed ; Al Omar, Suliman Y. ; Al Anazi, Mohamed S. ; Nyengaard, Jens R. ; Alwasel, Saleh. / Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians. In: Biological Bulletin. 2017 ; Vol. 232, No. 1. pp. 12-18.

Bibtex

@article{5d4f83f2609b4cbe9b9fda69c00801d2,
title = "Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians",
abstract = "The origin of infertility in the hyperplasic ovary of ex-fissiparous planarians remains poorly understood. In a previous study we demonstrated that a complex process of early autophagy, followed by apoptotic processes, occurs in the hyperplasic ovary of the freshwater planarian Dugesia arabica. The present study aimed to investigate whether the mRNA expression levels of selected mRNA-like genes are altered in the hyperplasic ovary of the ex-fissiparous freshwater planarian D. arabica compared to the normal ovary. Using human cytokine-specific primers including interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), we have successfully amplified by real-time polymerase chain reaction some transcripts that could be similar to those amplified in human. The transcript levels of the human-like transcript (IL-1-like and TNF-alpha-like) were significantly higher, 4.89- and 3.41-fold, respectively, in the hyperplasic ovary compared to the normal ovary (P <0.05). However, although IL-6-like levels were higher in the hyperplasic ovary than the normal ovary (2.57-fold), this difference was not significant (P > 0.05). Immunohistochemical labeling supported the quantitative real-time PCR, showing that, like their respective mRNA expression levels, IL-1, IL-6, and TNF-alpha-like proteins are more highly expressed in the hyperplasic ovary than in the normal ovary.",
keywords = "FRESH-WATER PLANARIANS, POLYCYSTIC-OVARY, TISSUE REGENERATION, FOLLICULAR-FLUID, TNF-ALPHA, TRICLADIDA, CELLS, PLATYHELMINTHES, INTERLEUKIN-33, IDENTIFICATION",
author = "Harrath, {Abdel Halim} and Abdelhabib Semlali and Lamjed Mansour and Waleed Aldahmash and {Al Omar}, {Suliman Y.} and {Al Anazi}, {Mohamed S.} and Nyengaard, {Jens R.} and Saleh Alwasel",
year = "2017",
month = feb,
doi = "10.1086/691408",
language = "English",
volume = "232",
pages = "12--18",
journal = "Biological Bulletin",
issn = "0006-3185",
publisher = "Chicago Univ. Press",
number = "1",

}

RIS

TY - JOUR

T1 - Dynamics of Cytokine-like Activity in the Hyperplasic Ovary of Ex-fissiparous Planarians

AU - Harrath, Abdel Halim

AU - Semlali, Abdelhabib

AU - Mansour, Lamjed

AU - Aldahmash, Waleed

AU - Al Omar, Suliman Y.

AU - Al Anazi, Mohamed S.

AU - Nyengaard, Jens R.

AU - Alwasel, Saleh

PY - 2017/2

Y1 - 2017/2

N2 - The origin of infertility in the hyperplasic ovary of ex-fissiparous planarians remains poorly understood. In a previous study we demonstrated that a complex process of early autophagy, followed by apoptotic processes, occurs in the hyperplasic ovary of the freshwater planarian Dugesia arabica. The present study aimed to investigate whether the mRNA expression levels of selected mRNA-like genes are altered in the hyperplasic ovary of the ex-fissiparous freshwater planarian D. arabica compared to the normal ovary. Using human cytokine-specific primers including interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), we have successfully amplified by real-time polymerase chain reaction some transcripts that could be similar to those amplified in human. The transcript levels of the human-like transcript (IL-1-like and TNF-alpha-like) were significantly higher, 4.89- and 3.41-fold, respectively, in the hyperplasic ovary compared to the normal ovary (P <0.05). However, although IL-6-like levels were higher in the hyperplasic ovary than the normal ovary (2.57-fold), this difference was not significant (P > 0.05). Immunohistochemical labeling supported the quantitative real-time PCR, showing that, like their respective mRNA expression levels, IL-1, IL-6, and TNF-alpha-like proteins are more highly expressed in the hyperplasic ovary than in the normal ovary.

AB - The origin of infertility in the hyperplasic ovary of ex-fissiparous planarians remains poorly understood. In a previous study we demonstrated that a complex process of early autophagy, followed by apoptotic processes, occurs in the hyperplasic ovary of the freshwater planarian Dugesia arabica. The present study aimed to investigate whether the mRNA expression levels of selected mRNA-like genes are altered in the hyperplasic ovary of the ex-fissiparous freshwater planarian D. arabica compared to the normal ovary. Using human cytokine-specific primers including interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), we have successfully amplified by real-time polymerase chain reaction some transcripts that could be similar to those amplified in human. The transcript levels of the human-like transcript (IL-1-like and TNF-alpha-like) were significantly higher, 4.89- and 3.41-fold, respectively, in the hyperplasic ovary compared to the normal ovary (P <0.05). However, although IL-6-like levels were higher in the hyperplasic ovary than the normal ovary (2.57-fold), this difference was not significant (P > 0.05). Immunohistochemical labeling supported the quantitative real-time PCR, showing that, like their respective mRNA expression levels, IL-1, IL-6, and TNF-alpha-like proteins are more highly expressed in the hyperplasic ovary than in the normal ovary.

KW - FRESH-WATER PLANARIANS

KW - POLYCYSTIC-OVARY

KW - TISSUE REGENERATION

KW - FOLLICULAR-FLUID

KW - TNF-ALPHA

KW - TRICLADIDA

KW - CELLS

KW - PLATYHELMINTHES

KW - INTERLEUKIN-33

KW - IDENTIFICATION

U2 - 10.1086/691408

DO - 10.1086/691408

M3 - Journal article

C2 - 28445093

VL - 232

SP - 12

EP - 18

JO - Biological Bulletin

JF - Biological Bulletin

SN - 0006-3185

IS - 1

ER -