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Jens Randel Nyengaard

Design-based stereological methods for estimating numbers of glomerular podocytes

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Design-based stereological methods for estimating numbers of glomerular podocytes. / Puelles, Victor G; Douglas-Denton, Rebecca N; Cullen-McEwen, Luise; McNamara, Bridgette J; Salih, Firuz; Li, Jinhua; Hughson, Michael D; Hoy, Wendy E; Nyengaard, Jens R; Bertram, John F.

In: Annals of Anatomy, Vol. 196, No. 1, 2014, p. 48-56.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Puelles, VG, Douglas-Denton, RN, Cullen-McEwen, L, McNamara, BJ, Salih, F, Li, J, Hughson, MD, Hoy, WE, Nyengaard, JR & Bertram, JF 2014, 'Design-based stereological methods for estimating numbers of glomerular podocytes', Annals of Anatomy, vol. 196, no. 1, pp. 48-56. https://doi.org/10.1016/j.aanat.2013.04.007

APA

Puelles, V. G., Douglas-Denton, R. N., Cullen-McEwen, L., McNamara, B. J., Salih, F., Li, J., Hughson, M. D., Hoy, W. E., Nyengaard, J. R., & Bertram, J. F. (2014). Design-based stereological methods for estimating numbers of glomerular podocytes. Annals of Anatomy, 196(1), 48-56. https://doi.org/10.1016/j.aanat.2013.04.007

CBE

Puelles VG, Douglas-Denton RN, Cullen-McEwen L, McNamara BJ, Salih F, Li J, Hughson MD, Hoy WE, Nyengaard JR, Bertram JF. 2014. Design-based stereological methods for estimating numbers of glomerular podocytes. Annals of Anatomy. 196(1):48-56. https://doi.org/10.1016/j.aanat.2013.04.007

MLA

Vancouver

Puelles VG, Douglas-Denton RN, Cullen-McEwen L, McNamara BJ, Salih F, Li J et al. Design-based stereological methods for estimating numbers of glomerular podocytes. Annals of Anatomy. 2014;196(1):48-56. https://doi.org/10.1016/j.aanat.2013.04.007

Author

Puelles, Victor G ; Douglas-Denton, Rebecca N ; Cullen-McEwen, Luise ; McNamara, Bridgette J ; Salih, Firuz ; Li, Jinhua ; Hughson, Michael D ; Hoy, Wendy E ; Nyengaard, Jens R ; Bertram, John F. / Design-based stereological methods for estimating numbers of glomerular podocytes. In: Annals of Anatomy. 2014 ; Vol. 196, No. 1. pp. 48-56.

Bibtex

@article{56ca8b5b7de44bc48dbf924351335756,
title = "Design-based stereological methods for estimating numbers of glomerular podocytes",
abstract = "The podocyte depletion hypothesis has emerged as a unifying concept in glomerular pathology. According to this hypothesis podocyte depletion may be absolute (decrease in number of healthy mature podocytes), relative (fewer podocytes per unit of glomerular volume) or involve alterations to the specialized podocyte architecture (such as foot process effacement). To study and understand podocyte depletion it is important to be able to accurately and precisely count these cells. Here we present new design-based stereological methods for estimating podocyte number in individual glomeruli of known volume, and in average glomeruli. Both methods involve serial histological sectioning, triple label immunohistochemistry, laser confocal microscopy and cell counting with the optical disector/fractionator.",
author = "Puelles, {Victor G} and Douglas-Denton, {Rebecca N} and Luise Cullen-McEwen and McNamara, {Bridgette J} and Firuz Salih and Jinhua Li and Hughson, {Michael D} and Hoy, {Wendy E} and Nyengaard, {Jens R} and Bertram, {John F}",
note = "Copyright {\textcopyright} 2013 Elsevier GmbH. All rights reserved.",
year = "2014",
doi = "10.1016/j.aanat.2013.04.007",
language = "English",
volume = "196",
pages = "48--56",
journal = "Annals of Anatomy",
issn = "0940-9602",
publisher = "Elsevier GmbH",
number = "1",

}

RIS

TY - JOUR

T1 - Design-based stereological methods for estimating numbers of glomerular podocytes

AU - Puelles, Victor G

AU - Douglas-Denton, Rebecca N

AU - Cullen-McEwen, Luise

AU - McNamara, Bridgette J

AU - Salih, Firuz

AU - Li, Jinhua

AU - Hughson, Michael D

AU - Hoy, Wendy E

AU - Nyengaard, Jens R

AU - Bertram, John F

N1 - Copyright © 2013 Elsevier GmbH. All rights reserved.

PY - 2014

Y1 - 2014

N2 - The podocyte depletion hypothesis has emerged as a unifying concept in glomerular pathology. According to this hypothesis podocyte depletion may be absolute (decrease in number of healthy mature podocytes), relative (fewer podocytes per unit of glomerular volume) or involve alterations to the specialized podocyte architecture (such as foot process effacement). To study and understand podocyte depletion it is important to be able to accurately and precisely count these cells. Here we present new design-based stereological methods for estimating podocyte number in individual glomeruli of known volume, and in average glomeruli. Both methods involve serial histological sectioning, triple label immunohistochemistry, laser confocal microscopy and cell counting with the optical disector/fractionator.

AB - The podocyte depletion hypothesis has emerged as a unifying concept in glomerular pathology. According to this hypothesis podocyte depletion may be absolute (decrease in number of healthy mature podocytes), relative (fewer podocytes per unit of glomerular volume) or involve alterations to the specialized podocyte architecture (such as foot process effacement). To study and understand podocyte depletion it is important to be able to accurately and precisely count these cells. Here we present new design-based stereological methods for estimating podocyte number in individual glomeruli of known volume, and in average glomeruli. Both methods involve serial histological sectioning, triple label immunohistochemistry, laser confocal microscopy and cell counting with the optical disector/fractionator.

U2 - 10.1016/j.aanat.2013.04.007

DO - 10.1016/j.aanat.2013.04.007

M3 - Journal article

C2 - 23845787

VL - 196

SP - 48

EP - 56

JO - Annals of Anatomy

JF - Annals of Anatomy

SN - 0940-9602

IS - 1

ER -