Jens Randel Nyengaard

Corrigendum to "Long-term valproic acid exposure increases the number of neocortical neurons in the developing rat brain" [Neurosci. Lett. 580 (2014) 12-16]: A possible new animal model of autism.

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Corrigendum to "Long-term valproic acid exposure increases the number of neocortical neurons in the developing rat brain" [Neurosci. Lett. 580 (2014) 12-16] : A possible new animal model of autism. / Sabers, Anne; Bertelsen, Freja C.B.; Scheel-Krüger, Jørgen; Nyengaard, Jens R.; Møller, Arne.

In: Neuroscience Letters, Vol. 588, 09.02.2015, p. 203-207.

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@article{4c3cddf404704bb6a8f4cf084d514db7,
title = "Corrigendum to {"}Long-term valproic acid exposure increases the number of neocortical neurons in the developing rat brain{"} [Neurosci. Lett. 580 (2014) 12-16]: A possible new animal model of autism.",
abstract = "The aim of this study was to test the hypothesis that long-term fetal valproic acid (VPA) exposure at doses relevant to the human clinic interferes with normal brain development. Pregnant rats were given intraperitoneal injections of VPA (20mg/kg or 100mg/kg) continuously during the last 9-12 days of pregnancy and during the lactation period until sacrifice on the 23rd postnatal day. Total number of neocortical neurons was estimated using the optical fractionator and frontal cortical thicknesses were sampled in VPA exposed pups compared with an unexposed control group. We found that pups exposed to 20mg/kg and 100mg/kg doses of VPA had statistically significant higher total number of neurons in neocortex by 15.8% and 12.3%, respectively, (p<0.05) compared to controls amounting to 15.5×106 neocortical neurons (p<0.01). There was no statistical difference between the two VPA groups. Pups exposed to 100mg/kg, but not to 20mg/kg VPA displayed a significant (p<0.05) broader (7.5%) of frontal cortical thickness compared to controls. Our results support the hypothesis that fetal exposure of VPA may interfere with normal brain development by disturbing neocortical organization, resulting in overgrowth of frontal lobes and increased neuronal cell numbers. The results indirectly suggest that prenatal VPA may contribute as a causative factor in the brain developmental disturbances equivalent to those seen in human autism spectrum disorders. We therefore suggest that this version of the VPA model may provide a translational model of autism.",
keywords = "Autism, Neurodevelopment, Pregnancy, Teratogenesis, Valproate, Valproic acid",
author = "Anne Sabers and Bertelsen, {Freja C.B.} and J{\o}rgen Scheel-Kr{\"u}ger and Nyengaard, {Jens R.} and Arne M{\o}ller",
year = "2015",
month = feb,
day = "9",
doi = "10.1016/j.neulet.2014.12.014",
language = "English",
volume = "588",
pages = "203--207",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd.",

}

RIS

TY - JOUR

T1 - Corrigendum to "Long-term valproic acid exposure increases the number of neocortical neurons in the developing rat brain" [Neurosci. Lett. 580 (2014) 12-16]

T2 - A possible new animal model of autism.

AU - Sabers, Anne

AU - Bertelsen, Freja C.B.

AU - Scheel-Krüger, Jørgen

AU - Nyengaard, Jens R.

AU - Møller, Arne

PY - 2015/2/9

Y1 - 2015/2/9

N2 - The aim of this study was to test the hypothesis that long-term fetal valproic acid (VPA) exposure at doses relevant to the human clinic interferes with normal brain development. Pregnant rats were given intraperitoneal injections of VPA (20mg/kg or 100mg/kg) continuously during the last 9-12 days of pregnancy and during the lactation period until sacrifice on the 23rd postnatal day. Total number of neocortical neurons was estimated using the optical fractionator and frontal cortical thicknesses were sampled in VPA exposed pups compared with an unexposed control group. We found that pups exposed to 20mg/kg and 100mg/kg doses of VPA had statistically significant higher total number of neurons in neocortex by 15.8% and 12.3%, respectively, (p<0.05) compared to controls amounting to 15.5×106 neocortical neurons (p<0.01). There was no statistical difference between the two VPA groups. Pups exposed to 100mg/kg, but not to 20mg/kg VPA displayed a significant (p<0.05) broader (7.5%) of frontal cortical thickness compared to controls. Our results support the hypothesis that fetal exposure of VPA may interfere with normal brain development by disturbing neocortical organization, resulting in overgrowth of frontal lobes and increased neuronal cell numbers. The results indirectly suggest that prenatal VPA may contribute as a causative factor in the brain developmental disturbances equivalent to those seen in human autism spectrum disorders. We therefore suggest that this version of the VPA model may provide a translational model of autism.

AB - The aim of this study was to test the hypothesis that long-term fetal valproic acid (VPA) exposure at doses relevant to the human clinic interferes with normal brain development. Pregnant rats were given intraperitoneal injections of VPA (20mg/kg or 100mg/kg) continuously during the last 9-12 days of pregnancy and during the lactation period until sacrifice on the 23rd postnatal day. Total number of neocortical neurons was estimated using the optical fractionator and frontal cortical thicknesses were sampled in VPA exposed pups compared with an unexposed control group. We found that pups exposed to 20mg/kg and 100mg/kg doses of VPA had statistically significant higher total number of neurons in neocortex by 15.8% and 12.3%, respectively, (p<0.05) compared to controls amounting to 15.5×106 neocortical neurons (p<0.01). There was no statistical difference between the two VPA groups. Pups exposed to 100mg/kg, but not to 20mg/kg VPA displayed a significant (p<0.05) broader (7.5%) of frontal cortical thickness compared to controls. Our results support the hypothesis that fetal exposure of VPA may interfere with normal brain development by disturbing neocortical organization, resulting in overgrowth of frontal lobes and increased neuronal cell numbers. The results indirectly suggest that prenatal VPA may contribute as a causative factor in the brain developmental disturbances equivalent to those seen in human autism spectrum disorders. We therefore suggest that this version of the VPA model may provide a translational model of autism.

KW - Autism

KW - Neurodevelopment

KW - Pregnancy

KW - Teratogenesis

KW - Valproate

KW - Valproic acid

UR - http://www.scopus.com/inward/record.url?scp=84921448568&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2014.12.014

DO - 10.1016/j.neulet.2014.12.014

M3 - Comment/debate

C2 - 26060869

AN - SCOPUS:84921448568

VL - 588

SP - 203

EP - 207

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

ER -