Jens Randel Nyengaard

Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity. / Moudi, Bita; Heidari, Zahra; Mahmoudzadeh-Sagheb, Hamidreza; Alavian, Seyed Moayed; Lankarani, Kamran B.; Farrokh, Parisa; Nyengaard, Jens Randel.

In: European Journal of Histochemistry, Vol. 62, No. 1, 2859, 29.01.2018, p. 42-50.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Moudi, B, Heidari, Z, Mahmoudzadeh-Sagheb, H, Alavian, SM, Lankarani, KB, Farrokh, P & Nyengaard, JR 2018, 'Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity', European Journal of Histochemistry, vol. 62, no. 1, 2859, pp. 42-50. https://doi.org/10.4081/ejh.2018.2859

APA

Moudi, B., Heidari, Z., Mahmoudzadeh-Sagheb, H., Alavian, S. M., Lankarani, K. B., Farrokh, P., & Nyengaard, J. R. (2018). Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity. European Journal of Histochemistry, 62(1), 42-50. [2859]. https://doi.org/10.4081/ejh.2018.2859

CBE

MLA

Vancouver

Moudi B, Heidari Z, Mahmoudzadeh-Sagheb H, Alavian SM, Lankarani KB, Farrokh P et al. Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity. European Journal of Histochemistry. 2018 Jan 29;62(1):42-50. 2859. https://doi.org/10.4081/ejh.2018.2859

Author

Moudi, Bita ; Heidari, Zahra ; Mahmoudzadeh-Sagheb, Hamidreza ; Alavian, Seyed Moayed ; Lankarani, Kamran B. ; Farrokh, Parisa ; Nyengaard, Jens Randel. / Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity. In: European Journal of Histochemistry. 2018 ; Vol. 62, No. 1. pp. 42-50.

Bibtex

@article{25e3a0973a3e418ab0a6a6445f4f278d,
title = "Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity",
abstract = "Hepatocellular carcinoma is the third leading cause of cancer-related death worldwide and late diagnosis is the main cause of death in HCC patients. In this study expression patterns of HSP70, GPC3 and GS and their relationships with pathogenesis of HCC in Iranian patients were investigated. The expression of HSP70, GPC3 and GS were determined by immunohistochemistry and quantitative real-time PCR (q-PCR) methods, using 121 cases from patients with HBV alone, HCC without HBV, HBV+HCC and 30 normal tissues as control group. HSP70, GPC3 and GS were expressed in higher levels in HBV-related HCC samples compared to HBV alone group. The results showed that the labeling index of HSP70, GPC3 and GS are correlated ith immunohistochemical and molecular expressions of HSP70, GPC3 and GS. The sensitivity and specificity for HCC diagnosis were 43.4% and 89.7% for HSP70, 64.3% and 90.4% for GPC3, and 60.7% and 94.3% for GS, respectively. The sensitivity and specificity of the panels with 3, 2 and 1 positive markers, regardless of which one, were 21.6% and 100%, 51.3% and 100% and 93.4% and 80.5% respectively. The current study demonstrated an association between HSP70, GPC3 and GS expressions and HBV-related HCC in our population. It was concluded that HSP70, GPC3 and GS expressions could be useful biomarkers for increasing the specificity and sensitivity of HCC diagnosis to acceptable level. Also, proper combinations of these 3 markers could improve diagnostic accuracy.",
keywords = "HSP70, GPC3, GS, hepatocellular carcinoma, immunohistochemistry, quantitative real-time PCR, BETA-CATENIN MUTATIONS, DYSPLASTIC NODULES, VIRUS-INFECTION, LIVER BIOPSIES, HEPATITIS-C, EXPRESSION, MARKER, LESIONS, AFLATOXIN",
author = "Bita Moudi and Zahra Heidari and Hamidreza Mahmoudzadeh-Sagheb and Alavian, {Seyed Moayed} and Lankarani, {Kamran B.} and Parisa Farrokh and Nyengaard, {Jens Randel}",
year = "2018",
month = jan,
day = "29",
doi = "10.4081/ejh.2018.2859",
language = "English",
volume = "62",
pages = "42--50",
journal = "European Journal of Histochemistry",
issn = "1121-760X",
publisher = "Pagepress",
number = "1",

}

RIS

TY - JOUR

T1 - Concomitant use of heat-shock protein 70, glutamine synthetase and glypican-3 is useful in diagnosis of HBV-related hepatocellular carcinoma with higher specificity and sensitivity

AU - Moudi, Bita

AU - Heidari, Zahra

AU - Mahmoudzadeh-Sagheb, Hamidreza

AU - Alavian, Seyed Moayed

AU - Lankarani, Kamran B.

AU - Farrokh, Parisa

AU - Nyengaard, Jens Randel

PY - 2018/1/29

Y1 - 2018/1/29

N2 - Hepatocellular carcinoma is the third leading cause of cancer-related death worldwide and late diagnosis is the main cause of death in HCC patients. In this study expression patterns of HSP70, GPC3 and GS and their relationships with pathogenesis of HCC in Iranian patients were investigated. The expression of HSP70, GPC3 and GS were determined by immunohistochemistry and quantitative real-time PCR (q-PCR) methods, using 121 cases from patients with HBV alone, HCC without HBV, HBV+HCC and 30 normal tissues as control group. HSP70, GPC3 and GS were expressed in higher levels in HBV-related HCC samples compared to HBV alone group. The results showed that the labeling index of HSP70, GPC3 and GS are correlated ith immunohistochemical and molecular expressions of HSP70, GPC3 and GS. The sensitivity and specificity for HCC diagnosis were 43.4% and 89.7% for HSP70, 64.3% and 90.4% for GPC3, and 60.7% and 94.3% for GS, respectively. The sensitivity and specificity of the panels with 3, 2 and 1 positive markers, regardless of which one, were 21.6% and 100%, 51.3% and 100% and 93.4% and 80.5% respectively. The current study demonstrated an association between HSP70, GPC3 and GS expressions and HBV-related HCC in our population. It was concluded that HSP70, GPC3 and GS expressions could be useful biomarkers for increasing the specificity and sensitivity of HCC diagnosis to acceptable level. Also, proper combinations of these 3 markers could improve diagnostic accuracy.

AB - Hepatocellular carcinoma is the third leading cause of cancer-related death worldwide and late diagnosis is the main cause of death in HCC patients. In this study expression patterns of HSP70, GPC3 and GS and their relationships with pathogenesis of HCC in Iranian patients were investigated. The expression of HSP70, GPC3 and GS were determined by immunohistochemistry and quantitative real-time PCR (q-PCR) methods, using 121 cases from patients with HBV alone, HCC without HBV, HBV+HCC and 30 normal tissues as control group. HSP70, GPC3 and GS were expressed in higher levels in HBV-related HCC samples compared to HBV alone group. The results showed that the labeling index of HSP70, GPC3 and GS are correlated ith immunohistochemical and molecular expressions of HSP70, GPC3 and GS. The sensitivity and specificity for HCC diagnosis were 43.4% and 89.7% for HSP70, 64.3% and 90.4% for GPC3, and 60.7% and 94.3% for GS, respectively. The sensitivity and specificity of the panels with 3, 2 and 1 positive markers, regardless of which one, were 21.6% and 100%, 51.3% and 100% and 93.4% and 80.5% respectively. The current study demonstrated an association between HSP70, GPC3 and GS expressions and HBV-related HCC in our population. It was concluded that HSP70, GPC3 and GS expressions could be useful biomarkers for increasing the specificity and sensitivity of HCC diagnosis to acceptable level. Also, proper combinations of these 3 markers could improve diagnostic accuracy.

KW - HSP70

KW - GPC3

KW - GS

KW - hepatocellular carcinoma

KW - immunohistochemistry

KW - quantitative real-time PCR

KW - BETA-CATENIN MUTATIONS

KW - DYSPLASTIC NODULES

KW - VIRUS-INFECTION

KW - LIVER BIOPSIES

KW - HEPATITIS-C

KW - EXPRESSION

KW - MARKER

KW - LESIONS

KW - AFLATOXIN

UR - http://www.scopus.com/inward/record.url?scp=85041527875&partnerID=8YFLogxK

U2 - 10.4081/ejh.2018.2859

DO - 10.4081/ejh.2018.2859

M3 - Journal article

C2 - 29569872

AN - SCOPUS:85041527875

VL - 62

SP - 42

EP - 50

JO - European Journal of Histochemistry

JF - European Journal of Histochemistry

SN - 1121-760X

IS - 1

M1 - 2859

ER -