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Jens Randel Nyengaard

Cellular expression of α7 nicotinic acetylcholine receptor protein in the temporal cortex in alzheimer’s and parkinson’s disease-a stereological approach

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DOI

  • Carolin Banerjee, Universitat zu Koln
  • ,
  • Jens R. Nyengaard
  • Andrea Wevers, Universitat zu Koln
  • ,
  • Rob A.I. De Vos, Laboratorium Pathologie Oost Nederland
  • ,
  • Ernst N.H.Jansen Steur, Medisch Spectrum Twente (MST)
  • ,
  • Jon Lindstrom, University of Pennsylvania, School of Medicine
  • ,
  • Kirsten Pilz, Universitat zu Koln
  • ,
  • Sonja Nowacki, Universitat zu Koln
  • ,
  • Wilhelm Bloch, Universitat zu Koln
  • ,
  • Hannsjörg Schröder, Universitat zu Koln

Cognitive deficits in Alzheimer's and Parkinson's disease are closely related to disturbed cholinergic transmission. The decrease of nicotinic acetylcholine receptor protein has been assessed by Western blotting and immunohistochemistry. Stereology, however, has not been used to assess numbers of receptor-expressing human cerebrocortical neurons. Our approach applies a combination of α7 subunit-immunohistochemistry with a stereological technique using defined stretches of pial surface as reference standard. The number of α7 subunit protein-expressing neurons in the Alzheimer temporal cortices amounted to approximately half of that of controls while numbers in Parkinson patients lay in between. No differences in the total number of neurons were seen. These results corroborate nonstereological studies on Alzheimer cortices and for the first time show a similar decrease in receptor expression in Parkinson's disease. They provide evidence that not only Alzheimer dementia but also cognitive deficits in Parkinson's disease may be related to decreased nicotinic receptor expression.

Original languageEnglish
JournalNeurobiology of Disease
Volume7
Issue6
Pages (from-to)666-672
Number of pages7
ISSN0969-9961
DOIs
Publication statusPublished - 1 Jan 2000

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