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Jens Randel Nyengaard

Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

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Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles. / Just, Jesper; Yan, Yan; Farup, Jean; Sieljacks, Peter; Sloth, Mette; Venø, Morten; Gu, Tingting; de Paoli, Frank Vincenzo; Nyengaard, Jens Randel; Bæk, Rikke; Jørgensen, Malene Møller; Kjems, Jørgen; Vissing, Kristian; Drasbek, Kim Ryun.

In: Scientific Reports, Vol. 10, No. 1, 5835, 2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{1c4235ef4af0458aa28357ef1ce0f70b,
title = "Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles",
abstract = "Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.",
author = "Jesper Just and Yan Yan and Jean Farup and Peter Sieljacks and Mette Sloth and Morten Ven{\o} and Tingting Gu and {de Paoli}, {Frank Vincenzo} and Nyengaard, {Jens Randel} and Rikke B{\ae}k and J{\o}rgensen, {Malene M{\o}ller} and J{\o}rgen Kjems and Kristian Vissing and Drasbek, {Kim Ryun}",
year = "2020",
doi = "10.1038/s41598-020-62456-3",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

AU - Just, Jesper

AU - Yan, Yan

AU - Farup, Jean

AU - Sieljacks, Peter

AU - Sloth, Mette

AU - Venø, Morten

AU - Gu, Tingting

AU - de Paoli, Frank Vincenzo

AU - Nyengaard, Jens Randel

AU - Bæk, Rikke

AU - Jørgensen, Malene Møller

AU - Kjems, Jørgen

AU - Vissing, Kristian

AU - Drasbek, Kim Ryun

PY - 2020

Y1 - 2020

N2 - Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.

AB - Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.

U2 - 10.1038/s41598-020-62456-3

DO - 10.1038/s41598-020-62456-3

M3 - Journal article

C2 - 32245988

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 5835

ER -