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Jens Randel Nyengaard

Application of stereological principles for quantification of bacteria in intact dental biofilms

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Quantative confocal laser scanning microscopy (CLSM) in combination with fluorescent in situ hybridization (FISH) may help to increase our knowledge about biofilm formation. The purpose of this study was to develop and evaluate a stereological method for quantification of bacteria in intact biofilm. The method was applied in a quantitative study of the proportion of streptococci relative to other bacteria in initial in-situ-grown dental biofilms as a function of time.
Original languageEnglish
JournalOral Microbiology and Immunology
Pages (from-to)69-75
Number of pages7
Publication statusPublished - 2009

    Research areas

  • Adult, Biofilms, Colony Count, Microbial, DNA, Bacterial, Dental Plaque, Female, Humans, Imaging, Three-Dimensional, In Situ Hybridization, Fluorescence, Male, Microscopy, Confocal, RNA, Ribosomal, 16S, Streptococcus, Young Adult

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