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Jens Randel Nyengaard

Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain

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Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. / Hundahl, Christian Ansgar; Allen, Gregg C; Hannibal, Jens; Kjaer, Katrine; Rehfeld, Jens F.; Dewilde, Sylvia; Nyengaard, Jens R; Kelsen, Jesper; Hay-Schmidt, Anders.

In: Brain Research, Vol. 1331, 17.05.2010, p. 58-73.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hundahl, CA, Allen, GC, Hannibal, J, Kjaer, K, Rehfeld, JF, Dewilde, S, Nyengaard, JR, Kelsen, J & Hay-Schmidt, A 2010, 'Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain', Brain Research, vol. 1331, pp. 58-73. https://doi.org/10.1016/j.brainres.2010.03.056

APA

Hundahl, C. A., Allen, G. C., Hannibal, J., Kjaer, K., Rehfeld, J. F., Dewilde, S., Nyengaard, J. R., Kelsen, J., & Hay-Schmidt, A. (2010). Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. Brain Research, 1331, 58-73. https://doi.org/10.1016/j.brainres.2010.03.056

CBE

Hundahl CA, Allen GC, Hannibal J, Kjaer K, Rehfeld JF, Dewilde S, Nyengaard JR, Kelsen J, Hay-Schmidt A. 2010. Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. Brain Research. 1331:58-73. https://doi.org/10.1016/j.brainres.2010.03.056

MLA

Vancouver

Hundahl CA, Allen GC, Hannibal J, Kjaer K, Rehfeld JF, Dewilde S et al. Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. Brain Research. 2010 May 17;1331:58-73. https://doi.org/10.1016/j.brainres.2010.03.056

Author

Hundahl, Christian Ansgar ; Allen, Gregg C ; Hannibal, Jens ; Kjaer, Katrine ; Rehfeld, Jens F. ; Dewilde, Sylvia ; Nyengaard, Jens R ; Kelsen, Jesper ; Hay-Schmidt, Anders. / Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. In: Brain Research. 2010 ; Vol. 1331. pp. 58-73.

Bibtex

@article{a6ed341c95c64a9c9cc714717f2f7e7e,
title = "Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain",
abstract = "The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.",
keywords = "Animals, Brain, Globins, Immunohistochemistry, In Situ Hybridization, Male, Mice, Mice, Inbred C57BL, Microscopy, Immunoelectron, Nerve Tissue Proteins, Neurons, Nitric Oxide, RNA, Messenger",
author = "Hundahl, {Christian Ansgar} and Allen, {Gregg C} and Jens Hannibal and Katrine Kjaer and Rehfeld, {Jens F.} and Sylvia Dewilde and Nyengaard, {Jens R} and Jesper Kelsen and Anders Hay-Schmidt",
note = "Copyright 2010 Elsevier B.V. All rights reserved.",
year = "2010",
month = may,
day = "17",
doi = "10.1016/j.brainres.2010.03.056",
language = "English",
volume = "1331",
pages = "58--73",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain

AU - Hundahl, Christian Ansgar

AU - Allen, Gregg C

AU - Hannibal, Jens

AU - Kjaer, Katrine

AU - Rehfeld, Jens F.

AU - Dewilde, Sylvia

AU - Nyengaard, Jens R

AU - Kelsen, Jesper

AU - Hay-Schmidt, Anders

N1 - Copyright 2010 Elsevier B.V. All rights reserved.

PY - 2010/5/17

Y1 - 2010/5/17

N2 - The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.

AB - The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.

KW - Animals

KW - Brain

KW - Globins

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Microscopy, Immunoelectron

KW - Nerve Tissue Proteins

KW - Neurons

KW - Nitric Oxide

KW - RNA, Messenger

U2 - 10.1016/j.brainres.2010.03.056

DO - 10.1016/j.brainres.2010.03.056

M3 - Journal article

C2 - 20331985

VL - 1331

SP - 58

EP - 73

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -