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Jens Randel Nyengaard

A handheld support system to facilitate stereological measurements and mapping of branching structures

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  • Jonathan Eyal Gardi, Denmark
  • Dvoralai Wulfsohn, Department of Agricultural Sciences - Section for Environment, Resources & Technology, Faculty of Life Sciences, University of Copenhagen, Tåstrup, Denmark
  • Jens Randel Nyengaard
  • Stereological Research Laboratory
  • Institute of Clinical Medicine
  • The Department of Pathology - ÅKH
‘BranchSampler' is a system for computer-assisted manual stereology written for handheld devices running Windows CE. The system has been designed specifically to streamline data collection and optimize sampling of tree-like branching structures, with particular aims of reducing user errors, saving time, and saving data in formats suited for further analysis in other software, e.g. a spreadsheet. The system can be applied in a wide range of applications, from biomedical science to agriculture and horticulture. It can be applied for sampling nested generations of lung bronchioles and renal arterioles or for collection and optimizing sampling of crops for precision agriculture. Although the system has been designed specifically for sampling branching structures, it is sufficiently flexible to be used for other applications involving nested stereological designs. We describe the system specifications, software and Graphical User Interface (GUI) development, functionality and application of the handheld system using four examples: (1) sampling monkey lung bronchioles for estimation of diameter and wall thickness (2) sampling rat kidney for estimating number of arteries and arterioles in a specific generation (3) mapping fruit (apple) tree yield in an orchard, and (4) estimating the total leaf surface area of chrysanthemum plants in a greenhouse.
Original languageEnglish
JournalJournal of Microscopy
Volume227
Issue2
Pages (from-to)124-139
Number of pages16
ISSN0022-2720
Publication statusPublished - 2007

    Research areas

  • Branching, bioscience, bronchioles, computer-assisted stereology, fractionator, handheld, microscopy, measurement, multi-stage sampling, precision agriculture, portable, trees, variance prediction, vessels.

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