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Jens Randel Nyengaard

A design-based method for estimating glomerular number in the developing kidney

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

A design-based method for estimating glomerular number in the developing kidney. / Cullen-McEwen, Luise Anne; Armitage, James Andrew; Nyengaard, Jens Randel; Moritz, Karen M; Bertram, John F.

In: American Journal of Physiology: Renal Physiology, 16.03.2011.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Cullen-McEwen, LA, Armitage, JA, Nyengaard, JR, Moritz, KM & Bertram, JF 2011, 'A design-based method for estimating glomerular number in the developing kidney', American Journal of Physiology: Renal Physiology. https://doi.org/10.1152/ajprenal.00055.2011

APA

Cullen-McEwen, L. A., Armitage, J. A., Nyengaard, J. R., Moritz, K. M., & Bertram, J. F. (2011). A design-based method for estimating glomerular number in the developing kidney. American Journal of Physiology: Renal Physiology. https://doi.org/10.1152/ajprenal.00055.2011

CBE

Cullen-McEwen LA, Armitage JA, Nyengaard JR, Moritz KM, Bertram JF. 2011. A design-based method for estimating glomerular number in the developing kidney. American Journal of Physiology: Renal Physiology. https://doi.org/10.1152/ajprenal.00055.2011

MLA

Cullen-McEwen, Luise Anne et al. "A design-based method for estimating glomerular number in the developing kidney". American Journal of Physiology: Renal Physiology. 2011. https://doi.org/10.1152/ajprenal.00055.2011

Vancouver

Cullen-McEwen LA, Armitage JA, Nyengaard JR, Moritz KM, Bertram JF. A design-based method for estimating glomerular number in the developing kidney. American Journal of Physiology: Renal Physiology. 2011 Mar 16. https://doi.org/10.1152/ajprenal.00055.2011

Author

Cullen-McEwen, Luise Anne ; Armitage, James Andrew ; Nyengaard, Jens Randel ; Moritz, Karen M ; Bertram, John F. / A design-based method for estimating glomerular number in the developing kidney. In: American Journal of Physiology: Renal Physiology. 2011.

Bibtex

@article{b407e5192cbf4e228e343bf1cbdd4c82,
title = "A design-based method for estimating glomerular number in the developing kidney",
abstract = "Low glomerular (nephron) endowment has been associated with increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 weeks gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was in part due to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here we describe a method that uses lectin histochemistry to identify developing glomeruli, and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number (N(glom)). We have characterized N(glom) throughout development in kidneys from 76 rats and model this development with a five parameter logistic equation to predict N(glom) from embryonic day 17.25 to adulthood (r2=0.98). This approach represents the first design-based method with which to estimate N(glom) in the developing kidney.",
author = "Cullen-McEwen, {Luise Anne} and Armitage, {James Andrew} and Nyengaard, {Jens Randel} and Moritz, {Karen M} and Bertram, {John F}",
year = "2011",
month = mar,
day = "16",
doi = "10.1152/ajprenal.00055.2011",
language = "English",
journal = "American Journal of Physiology: Renal Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",

}

RIS

TY - JOUR

T1 - A design-based method for estimating glomerular number in the developing kidney

AU - Cullen-McEwen, Luise Anne

AU - Armitage, James Andrew

AU - Nyengaard, Jens Randel

AU - Moritz, Karen M

AU - Bertram, John F

PY - 2011/3/16

Y1 - 2011/3/16

N2 - Low glomerular (nephron) endowment has been associated with increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 weeks gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was in part due to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here we describe a method that uses lectin histochemistry to identify developing glomeruli, and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number (N(glom)). We have characterized N(glom) throughout development in kidneys from 76 rats and model this development with a five parameter logistic equation to predict N(glom) from embryonic day 17.25 to adulthood (r2=0.98). This approach represents the first design-based method with which to estimate N(glom) in the developing kidney.

AB - Low glomerular (nephron) endowment has been associated with increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 weeks gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was in part due to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here we describe a method that uses lectin histochemistry to identify developing glomeruli, and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number (N(glom)). We have characterized N(glom) throughout development in kidneys from 76 rats and model this development with a five parameter logistic equation to predict N(glom) from embryonic day 17.25 to adulthood (r2=0.98). This approach represents the first design-based method with which to estimate N(glom) in the developing kidney.

U2 - 10.1152/ajprenal.00055.2011

DO - 10.1152/ajprenal.00055.2011

M3 - Journal article

C2 - 21411478

JO - American Journal of Physiology: Renal Physiology

JF - American Journal of Physiology: Renal Physiology

SN - 1931-857X

ER -