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Jens Grønbech Hansen

Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis

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Standard

Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. / Walter, Stephanie; Ali, Sajid; Kemen, Eric; Nazari, Kumarse; Bahri, Bochra A; Enjalbert, Jérôme; Hansen, Jens Grønbech; Brown, James K M; Sicheritz-Ponten, Thomas; Jones, Jonathan; de Vallavieille-Pope, Claude; Hovmøller, Mogens S; Justesen, Annemarie F.

In: Ecology and Evolution, Vol. 6, No. 9, 20.03.2016, p. 2790-2804.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Walter, S, Ali, S, Kemen, E, Nazari, K, Bahri, BA, Enjalbert, J, Hansen, JG, Brown, JKM, Sicheritz-Ponten, T, Jones, J, de Vallavieille-Pope, C, Hovmøller, MS & Justesen, AF 2016, 'Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis', Ecology and Evolution, vol. 6, no. 9, pp. 2790-2804. https://doi.org/10.1002/ece3.2069

APA

Walter, S., Ali, S., Kemen, E., Nazari, K., Bahri, B. A., Enjalbert, J., Hansen, J. G., Brown, J. K. M., Sicheritz-Ponten, T., Jones, J., de Vallavieille-Pope, C., Hovmøller, M. S., & Justesen, A. F. (2016). Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. Ecology and Evolution, 6(9), 2790-2804. https://doi.org/10.1002/ece3.2069

CBE

Walter S, Ali S, Kemen E, Nazari K, Bahri BA, Enjalbert J, Hansen JG, Brown JKM, Sicheritz-Ponten T, Jones J, de Vallavieille-Pope C, Hovmøller MS, Justesen AF. 2016. Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. Ecology and Evolution. 6(9):2790-2804. https://doi.org/10.1002/ece3.2069

MLA

Vancouver

Walter S, Ali S, Kemen E, Nazari K, Bahri BA, Enjalbert J et al. Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. Ecology and Evolution. 2016 Mar 20;6(9):2790-2804. https://doi.org/10.1002/ece3.2069

Author

Walter, Stephanie ; Ali, Sajid ; Kemen, Eric ; Nazari, Kumarse ; Bahri, Bochra A ; Enjalbert, Jérôme ; Hansen, Jens Grønbech ; Brown, James K M ; Sicheritz-Ponten, Thomas ; Jones, Jonathan ; de Vallavieille-Pope, Claude ; Hovmøller, Mogens S ; Justesen, Annemarie F. / Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis. In: Ecology and Evolution. 2016 ; Vol. 6, No. 9. pp. 2790-2804.

Bibtex

@article{a7f6e6e12c6c4141a0fd85f614e2d7c8,
title = "Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis",
abstract = "Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.",
keywords = "aggressive strain, PCR-based markers, plant pathogen, wheat yellow rust/stripe rust",
author = "Stephanie Walter and Sajid Ali and Eric Kemen and Kumarse Nazari and Bahri, {Bochra A} and J{\'e}r{\^o}me Enjalbert and Hansen, {Jens Gr{\o}nbech} and Brown, {James K M} and Thomas Sicheritz-Ponten and Jonathan Jones and {de Vallavieille-Pope}, Claude and Hovm{\o}ller, {Mogens S} and Justesen, {Annemarie F}",
year = "2016",
month = mar,
day = "20",
doi = "10.1002/ece3.2069",
language = "English",
volume = "6",
pages = "2790--2804",
journal = "Ecology and Evolution",
issn = "2045-7758",
publisher = "John Wiley Sons Ltd",
number = "9",

}

RIS

TY - JOUR

T1 - Molecular markers for tracking the origin and worldwide distribution of invasive strains of Puccinia striiformis

AU - Walter, Stephanie

AU - Ali, Sajid

AU - Kemen, Eric

AU - Nazari, Kumarse

AU - Bahri, Bochra A

AU - Enjalbert, Jérôme

AU - Hansen, Jens Grønbech

AU - Brown, James K M

AU - Sicheritz-Ponten, Thomas

AU - Jones, Jonathan

AU - de Vallavieille-Pope, Claude

AU - Hovmøller, Mogens S

AU - Justesen, Annemarie F

PY - 2016/3/20

Y1 - 2016/3/20

N2 - Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.

AB - Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence-characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2.

KW - aggressive strain

KW - PCR-based markers

KW - plant pathogen

KW - wheat yellow rust/stripe rust

U2 - 10.1002/ece3.2069

DO - 10.1002/ece3.2069

M3 - Journal article

C2 - 27066253

VL - 6

SP - 2790

EP - 2804

JO - Ecology and Evolution

JF - Ecology and Evolution

SN - 2045-7758

IS - 9

ER -