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Jens Grønbech Hansen

Barberry rust survey – developing tools for diagnosis, analysis and data management

Research output: Contribution to conferencePosterResearch

Standard

Barberry rust survey – developing tools for diagnosis, analysis and data management. / Justesen, Annemarie Fejer; Hansen, Jens Grønbech; Hovmøller, Mogens Støvring; Lassen, Poul; Rodriguez Algaba, Julian; Hodson, D P; Patpour, Mehran; Nasab, H.R.; Aslanova, K.; Rahmatov, M.; Sikharulidze, Z.; Nazari, Kumarse.

2013. Poster session presented at Borlaug Global Rust Initiative (BGRI) Technical Workshop , New Delhi, India.

Research output: Contribution to conferencePosterResearch

Harvard

Justesen, AF, Hansen, JG, Hovmøller, MS, Lassen, P, Rodriguez Algaba, J, Hodson, DP, Patpour, M, Nasab, HR, Aslanova, K, Rahmatov, M, Sikharulidze, Z & Nazari, K 2013, 'Barberry rust survey – developing tools for diagnosis, analysis and data management', Borlaug Global Rust Initiative (BGRI) Technical Workshop , New Delhi, India, 19/08/2013.

APA

Justesen, A. F., Hansen, J. G., Hovmøller, M. S., Lassen, P., Rodriguez Algaba, J., Hodson, D. P., Patpour, M., Nasab, H. R., Aslanova, K., Rahmatov, M., Sikharulidze, Z., & Nazari, K. (2013). Barberry rust survey – developing tools for diagnosis, analysis and data management. Poster session presented at Borlaug Global Rust Initiative (BGRI) Technical Workshop , New Delhi, India.

CBE

Justesen AF, Hansen JG, Hovmøller MS, Lassen P, Rodriguez Algaba J, Hodson DP, Patpour M, Nasab HR, Aslanova K, Rahmatov M, Sikharulidze Z, Nazari K. 2013. Barberry rust survey – developing tools for diagnosis, analysis and data management. Poster session presented at Borlaug Global Rust Initiative (BGRI) Technical Workshop , New Delhi, India.

MLA

Justesen, Annemarie Fejer et al. Barberry rust survey – developing tools for diagnosis, analysis and data management. Borlaug Global Rust Initiative (BGRI) Technical Workshop , 19 Aug 2013, New Delhi, India, Poster, 2013. 1 p.

Vancouver

Justesen AF, Hansen JG, Hovmøller MS, Lassen P, Rodriguez Algaba J, Hodson DP et al. Barberry rust survey – developing tools for diagnosis, analysis and data management. 2013. Poster session presented at Borlaug Global Rust Initiative (BGRI) Technical Workshop , New Delhi, India.

Author

Justesen, Annemarie Fejer ; Hansen, Jens Grønbech ; Hovmøller, Mogens Støvring ; Lassen, Poul ; Rodriguez Algaba, Julian ; Hodson, D P ; Patpour, Mehran ; Nasab, H.R. ; Aslanova, K. ; Rahmatov, M. ; Sikharulidze, Z. ; Nazari, Kumarse. / Barberry rust survey – developing tools for diagnosis, analysis and data management. Poster session presented at Borlaug Global Rust Initiative (BGRI) Technical Workshop , New Delhi, India.1 p.

Bibtex

@conference{15905c15a2ed4995bcf36fe46b992763,
title = "Barberry rust survey – developing tools for diagnosis, analysis and data management",
abstract = "Barberry (Berberis spp.) may serve as alternate host of several Puccinia species including Puccinia graminis and P. striiformis causing stem and yellow rust on cereals and grasses, respectively. In order to study the importance of barberry in the epidemiology of Puccinia species in the CWANA region a rust survey was initiated. The aim was to 1) develop a surveillance protocol 2) develop molecular diagnostic tools for identifying Puccinia spp. from aecial samples, and 3) develop a data management and display system of results as part of the Wheat Rust ToolBox (http://wheatrust.org/international-services/service/). Rust infected leaves representing 120 barberry plants were collected in 2011 in Tajikistan and in 2012 in Iran, Azerbaijan, Uzbekistan and Georgia. Dried leaf samples were received at Global Rust Reference Center and DNA was extracted from up to six individual aecial clusters from each barberry plant. Due to variable quality of aecial samples DNA extraction was not successful for 40% of the samples. Sequences of EF1α, β-tubulin or ITS were analysed and compared to reference sequences of rust fungi infecting cereals and grasses. The analysis supported the presence of P. graminis s.l., P. arrhenatheri and P. striiformoides on barberry species. Survey and DNA sample maps with species designation were displayed in the Wheat Rust ToolBox. The future aim is to integrate barberry rust survey data based on molecular diagnostics and infection assays from research groups world-wide in order to gain insights into the role of barberry in the epidemiology of Puccinia spp. infecting cereals and grasses.",
author = "Justesen, {Annemarie Fejer} and Hansen, {Jens Gr{\o}nbech} and Hovm{\o}ller, {Mogens St{\o}vring} and Poul Lassen and {Rodriguez Algaba}, Julian and Hodson, {D P} and Mehran Patpour and H.R. Nasab and K. Aslanova and M. Rahmatov and Z. Sikharulidze and Kumarse Nazari",
year = "2013",
language = "English",
note = "Borlaug Global Rust Initiative (BGRI) Technical Workshop ; Conference date: 19-08-2013",

}

RIS

TY - CONF

T1 - Barberry rust survey – developing tools for diagnosis, analysis and data management

AU - Justesen, Annemarie Fejer

AU - Hansen, Jens Grønbech

AU - Hovmøller, Mogens Støvring

AU - Lassen, Poul

AU - Rodriguez Algaba, Julian

AU - Hodson, D P

AU - Patpour, Mehran

AU - Nasab, H.R.

AU - Aslanova, K.

AU - Rahmatov, M.

AU - Sikharulidze, Z.

AU - Nazari, Kumarse

PY - 2013

Y1 - 2013

N2 - Barberry (Berberis spp.) may serve as alternate host of several Puccinia species including Puccinia graminis and P. striiformis causing stem and yellow rust on cereals and grasses, respectively. In order to study the importance of barberry in the epidemiology of Puccinia species in the CWANA region a rust survey was initiated. The aim was to 1) develop a surveillance protocol 2) develop molecular diagnostic tools for identifying Puccinia spp. from aecial samples, and 3) develop a data management and display system of results as part of the Wheat Rust ToolBox (http://wheatrust.org/international-services/service/). Rust infected leaves representing 120 barberry plants were collected in 2011 in Tajikistan and in 2012 in Iran, Azerbaijan, Uzbekistan and Georgia. Dried leaf samples were received at Global Rust Reference Center and DNA was extracted from up to six individual aecial clusters from each barberry plant. Due to variable quality of aecial samples DNA extraction was not successful for 40% of the samples. Sequences of EF1α, β-tubulin or ITS were analysed and compared to reference sequences of rust fungi infecting cereals and grasses. The analysis supported the presence of P. graminis s.l., P. arrhenatheri and P. striiformoides on barberry species. Survey and DNA sample maps with species designation were displayed in the Wheat Rust ToolBox. The future aim is to integrate barberry rust survey data based on molecular diagnostics and infection assays from research groups world-wide in order to gain insights into the role of barberry in the epidemiology of Puccinia spp. infecting cereals and grasses.

AB - Barberry (Berberis spp.) may serve as alternate host of several Puccinia species including Puccinia graminis and P. striiformis causing stem and yellow rust on cereals and grasses, respectively. In order to study the importance of barberry in the epidemiology of Puccinia species in the CWANA region a rust survey was initiated. The aim was to 1) develop a surveillance protocol 2) develop molecular diagnostic tools for identifying Puccinia spp. from aecial samples, and 3) develop a data management and display system of results as part of the Wheat Rust ToolBox (http://wheatrust.org/international-services/service/). Rust infected leaves representing 120 barberry plants were collected in 2011 in Tajikistan and in 2012 in Iran, Azerbaijan, Uzbekistan and Georgia. Dried leaf samples were received at Global Rust Reference Center and DNA was extracted from up to six individual aecial clusters from each barberry plant. Due to variable quality of aecial samples DNA extraction was not successful for 40% of the samples. Sequences of EF1α, β-tubulin or ITS were analysed and compared to reference sequences of rust fungi infecting cereals and grasses. The analysis supported the presence of P. graminis s.l., P. arrhenatheri and P. striiformoides on barberry species. Survey and DNA sample maps with species designation were displayed in the Wheat Rust ToolBox. The future aim is to integrate barberry rust survey data based on molecular diagnostics and infection assays from research groups world-wide in order to gain insights into the role of barberry in the epidemiology of Puccinia spp. infecting cereals and grasses.

M3 - Poster

T2 - Borlaug Global Rust Initiative (BGRI) Technical Workshop

Y2 - 19 August 2013

ER -