Aarhus University Seal / Aarhus Universitets segl

Jens Christian Jensenius

VH and L-chain allotype determinants of rabbit IgG and IgA estimated by a semi-automatic, modified Farr-type radioimmunoassay;

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A sensitive radioimmunoassay for rabbit immunoglobulin allotypes has been developed. Separation of antibody-bound and free antigen was accomplished by salt precipitation with ammonium sulfate, followed by collection and wash on glass fiber filters by means of a semi-automatic cell harvester. The efficiency of the collection was increased by the addition of nonionic detergent which was salted out together with the precipitated reagents. The presentation of the a 1 allotype, located in the VH region, was found to be different on IgG and IgA molecules.
Original languageEnglish
JournalEuropean Journal of Immunology
Pages (from-to)99-103
Number of pages5
Publication statusPublished - 1977

    Research areas

  • Animals, Binding, Competitive, Chemical Precipitation, Epitopes, Immune Sera, Immunogenetics, Immunoglobulin A, Immunoglobulin Allotypes, Immunoglobulin Fab Fragments, Immunoglobulin G, Rabbits, Radioimmunoassay

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