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VH and L-chain allotype determinants of rabbit IgG and Ig...
About Aarhus University
Jens Christian Jensenius
VH and L-chain allotype determinants of rabbit IgG and IgA estimated by a semi-automatic, modified Farr-type radioimmunoassay;
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
A sensitive radioimmunoassay for rabbit immunoglobulin allotypes has been developed. Separation of antibody-bound and free antigen was accomplished by salt precipitation with ammonium sulfate, followed by collection and wash on glass fiber filters by means of a semi-automatic cell harvester. The efficiency of the collection was increased by the addition of nonionic detergent which was salted out together with the precipitated reagents. The presentation of the a 1 allotype, located in the VH region, was found to be different on IgG and IgA molecules.
| Original language | English |
|---|---|
| Journal | European Journal of Immunology |
| Volume | 7 |
| Issue | 2 |
| Pages (from-to) | 99-103 |
| Number of pages | 5 |
| ISSN | 0014-2980 |
| DOIs | |
| Publication status | Published - 1977 |
- Animals, Binding, Competitive, Chemical Precipitation, Epitopes, Immune Sera, Immunogenetics, Immunoglobulin A, Immunoglobulin Allotypes, Immunoglobulin Fab Fragments, Immunoglobulin G, Rabbits, Radioimmunoassay
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ID: 43924881