Aarhus University Seal / Aarhus Universitets segl

Jens Christian Jensenius

Serum levels, ontogeny and heritability of chicken mannan-binding lectin (MBL)

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  • Department of Medical Microbiology and Immunology
Mannan-binding lectin (MBL) is a serum lectin found in mammals and recently also in birds. It is thought to play an important role in the innate immune defence through binding to surface carbohydrates on micro-organisms followed by complement activation via the MBL pathway. This results in opsonization or direct complement-mediated killing. To gain further knowledge about the physiology and function of the protein, we developed an enzyme-linked immunosorbent assay for chicken MBL and used this to investigate the level of MBL in different chicken strains during embryogenesis, early and adult life. The MBL concentrations in 308 chickens, representing 14 different strains, showed a non-Gaussian, unimodal distribution profile with a mean concentration of 5.8 micrograms/ml (range 0.4-37.8 micrograms/ml). No difference between the strains could be demonstrated and no chickens were found deficient in MBL. Ontogenetic studies showed that MBL is already detectable in embryos at a gestational age of 10 days (11 days before hatching). At hatching, the level is comparable to the level found in adult chickens. This level is fairly stable during the first weeks of life, but a deficiency state develops at 4 weeks of age, whereafter the level is normalized again at 5 weeks of age. Chickens with relatively low or high MBL levels were bred with cockerels having similar MBL levels and this resulted in F1 generations with significantly different MBL levels, suggesting that the protein level is genetically influenced.
Original languageEnglish
JournalImmunology
Volume94
Issue4
Pages (from-to)587-93
Number of pages7
ISSN0019-2805
Publication statusPublished - 1 Aug 1998

    Research areas

  • Animals, Animals, Newborn, Breeding, Carrier Proteins, Chick Embryo, Chickens, Chromatography, Gel, Collectins, Enzyme-Linked Immunosorbent Assay, Female, Gestational Age, Hybridization, Genetic, Male

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