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Jens Christian Jensenius

Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)

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Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2). / Sallenbach, Seraina; Thiel, Steffen; Aebi, Christoph; Otth, Margrith; Bigler, Susanna; Jensenius, Jens Christian; Schlapbach, Luregn J; Ammann, Roland A.

In: Pediatric Allergy and Immunology, Vol. 22, No. 4, 2011, p. 424-30.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Sallenbach, Seraina ; Thiel, Steffen ; Aebi, Christoph ; Otth, Margrith ; Bigler, Susanna ; Jensenius, Jens Christian ; Schlapbach, Luregn J ; Ammann, Roland A. / Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2). In: Pediatric Allergy and Immunology. 2011 ; Vol. 22, No. 4. pp. 424-30.

Bibtex

@article{7e3eb61501354a72979a829b465c7a90,
title = "Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)",
abstract = "This study aimed to measure serum concentrations of five lectin-pathway components, mannan-binding lectin (MBL), M-ficolin, L-ficolin, H-ficolin, and MBL-associated serine protease-2 (MASP-2), in healthy neonates and children, to determine if they change with age and to compare them with serum concentrations in healthy adults. Concentrations were measured in 141 preterm and 30 term neonates, in 120 children including infants and adolescents, and in 350 adults (97 for L-ficolin) by inhouse time-resolved immunofluorometric assays or commercially available enzyme-linked immunosorbent assays. The adjacent categories method applying Wilcoxon-Mann-Whitney tests was used to determine age categories where concentrations differed significantly. Displaying serum concentration vs. age, an inverted-U shape (higher concentrations in children than in neonates and adults) was found for MBL and the ficolins, and an S-shape for MASP-2. Serum concentrations of all five lectin-pathway components were significantly lower in preterm neonates 1 yr and H-ficolin in term neonates and in children were found to be comparable with adult values. MBL, M-, L-, and H-ficolin, and MASP-2 serum concentrations show important changes with age. The respective normal ranges for adults should not be used in the pediatric population. The age-specific pediatric ranges established here may be used instead.",
keywords = "Adolescent, Adult, Age Factors, Child, Complement Pathway, Mannose-Binding Lectin, Diagnostic Errors, Female, Humans, Infant, Newborn, Lectins, Male, Mannose-Binding Lectin, Mannose-Binding Protein-Associated Serine Proteases, Pregnancy, Premature Birth",
author = "Seraina Sallenbach and Steffen Thiel and Christoph Aebi and Margrith Otth and Susanna Bigler and Jensenius, {Jens Christian} and Schlapbach, {Luregn J} and Ammann, {Roland A}",
note = "{\circledC} 2011 John Wiley & Sons A/S.",
year = "2011",
doi = "10.1111/j.1399-3038.2010.01104.x",
language = "English",
volume = "22",
pages = "424--30",
journal = "Pediatric Allergy and Immunology",
issn = "0905-6157",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)

AU - Sallenbach, Seraina

AU - Thiel, Steffen

AU - Aebi, Christoph

AU - Otth, Margrith

AU - Bigler, Susanna

AU - Jensenius, Jens Christian

AU - Schlapbach, Luregn J

AU - Ammann, Roland A

N1 - © 2011 John Wiley & Sons A/S.

PY - 2011

Y1 - 2011

N2 - This study aimed to measure serum concentrations of five lectin-pathway components, mannan-binding lectin (MBL), M-ficolin, L-ficolin, H-ficolin, and MBL-associated serine protease-2 (MASP-2), in healthy neonates and children, to determine if they change with age and to compare them with serum concentrations in healthy adults. Concentrations were measured in 141 preterm and 30 term neonates, in 120 children including infants and adolescents, and in 350 adults (97 for L-ficolin) by inhouse time-resolved immunofluorometric assays or commercially available enzyme-linked immunosorbent assays. The adjacent categories method applying Wilcoxon-Mann-Whitney tests was used to determine age categories where concentrations differed significantly. Displaying serum concentration vs. age, an inverted-U shape (higher concentrations in children than in neonates and adults) was found for MBL and the ficolins, and an S-shape for MASP-2. Serum concentrations of all five lectin-pathway components were significantly lower in preterm neonates 1 yr and H-ficolin in term neonates and in children were found to be comparable with adult values. MBL, M-, L-, and H-ficolin, and MASP-2 serum concentrations show important changes with age. The respective normal ranges for adults should not be used in the pediatric population. The age-specific pediatric ranges established here may be used instead.

AB - This study aimed to measure serum concentrations of five lectin-pathway components, mannan-binding lectin (MBL), M-ficolin, L-ficolin, H-ficolin, and MBL-associated serine protease-2 (MASP-2), in healthy neonates and children, to determine if they change with age and to compare them with serum concentrations in healthy adults. Concentrations were measured in 141 preterm and 30 term neonates, in 120 children including infants and adolescents, and in 350 adults (97 for L-ficolin) by inhouse time-resolved immunofluorometric assays or commercially available enzyme-linked immunosorbent assays. The adjacent categories method applying Wilcoxon-Mann-Whitney tests was used to determine age categories where concentrations differed significantly. Displaying serum concentration vs. age, an inverted-U shape (higher concentrations in children than in neonates and adults) was found for MBL and the ficolins, and an S-shape for MASP-2. Serum concentrations of all five lectin-pathway components were significantly lower in preterm neonates 1 yr and H-ficolin in term neonates and in children were found to be comparable with adult values. MBL, M-, L-, and H-ficolin, and MASP-2 serum concentrations show important changes with age. The respective normal ranges for adults should not be used in the pediatric population. The age-specific pediatric ranges established here may be used instead.

KW - Adolescent

KW - Adult

KW - Age Factors

KW - Child

KW - Complement Pathway, Mannose-Binding Lectin

KW - Diagnostic Errors

KW - Female

KW - Humans

KW - Infant, Newborn

KW - Lectins

KW - Male

KW - Mannose-Binding Lectin

KW - Mannose-Binding Protein-Associated Serine Proteases

KW - Pregnancy

KW - Premature Birth

U2 - 10.1111/j.1399-3038.2010.01104.x

DO - 10.1111/j.1399-3038.2010.01104.x

M3 - Journal article

VL - 22

SP - 424

EP - 430

JO - Pediatric Allergy and Immunology

JF - Pediatric Allergy and Immunology

SN - 0905-6157

IS - 4

ER -