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Jens Christian Jensenius

Quantification of human immunoglobulins by semiautomatic polyethylene glycol precipitation radioimmunoassays. Estimation of circulating immune complexes and immunoglobulin synthesized in vitro

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Quantification of human immunoglobulins by semiautomatic polyethylene glycol precipitation radioimmunoassays. Estimation of circulating immune complexes and immunoglobulin synthesized in vitro. / Jensenius, Jens Christian; Siersted, Hans Christian; Johnstone, A P.

In: Journal of Immunological Methods, Vol. 56, No. 1, 1983, p. 19-32.

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@article{d08a0f5a59c14f9c9301349779fca477,
title = "Quantification of human immunoglobulins by semiautomatic polyethylene glycol precipitation radioimmunoassays. Estimation of circulating immune complexes and immunoglobulin synthesized in vitro",
abstract = "Simple, reliable semiautomatic radioimmunoassays have been developed for the measurement of human immunoglobulins (IgG, IgA, IgM, IgK and Ig lambda). The assays are based on the separation of free from antibody-bound radiolabelled fragments of immunoglobulin by precipitation with 13% polyethylene glycol. The precipitate is harvested and washed on glass fibre filters by means of a commercial cell harvester. Radiolabelled lambda chain from Bence Jones urine was used for lambda chain assay and Fab' gamma from pooled IgG for the kappa assay. Labelled Fab or Fc fragments of IgG, IgA and IgM were used for the class specific assays. Selected commercial antisera were used throughout. The sensitivity limits of the assays performed according to the standard procedure were about 20 ng Ig per ml. The assays have been used for the quantification of circulating immune complexes and both cellular and secreted immunoglobulin produced in cell cultures. The results demonstrate the importance of the simultaneous quantification by L chain and H chain specific assays.",
keywords = "Animals, Antigen-Antibody Complex, Chemical Precipitation, Chickens, Detergents, Humans, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Immunoglobulin kappa-Chains, Immunoglobulin lambda-Chains, Immunoglobulins, Polyethylene Glycols, Rabbits, Radioimmunoassay",
author = "Jensenius, {Jens Christian} and Siersted, {Hans Christian} and Johnstone, {A P}",
year = "1983",
language = "English",
volume = "56",
pages = "19--32",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier BV",
number = "1",

}

RIS

TY - JOUR

T1 - Quantification of human immunoglobulins by semiautomatic polyethylene glycol precipitation radioimmunoassays. Estimation of circulating immune complexes and immunoglobulin synthesized in vitro

AU - Jensenius, Jens Christian

AU - Siersted, Hans Christian

AU - Johnstone, A P

PY - 1983

Y1 - 1983

N2 - Simple, reliable semiautomatic radioimmunoassays have been developed for the measurement of human immunoglobulins (IgG, IgA, IgM, IgK and Ig lambda). The assays are based on the separation of free from antibody-bound radiolabelled fragments of immunoglobulin by precipitation with 13% polyethylene glycol. The precipitate is harvested and washed on glass fibre filters by means of a commercial cell harvester. Radiolabelled lambda chain from Bence Jones urine was used for lambda chain assay and Fab' gamma from pooled IgG for the kappa assay. Labelled Fab or Fc fragments of IgG, IgA and IgM were used for the class specific assays. Selected commercial antisera were used throughout. The sensitivity limits of the assays performed according to the standard procedure were about 20 ng Ig per ml. The assays have been used for the quantification of circulating immune complexes and both cellular and secreted immunoglobulin produced in cell cultures. The results demonstrate the importance of the simultaneous quantification by L chain and H chain specific assays.

AB - Simple, reliable semiautomatic radioimmunoassays have been developed for the measurement of human immunoglobulins (IgG, IgA, IgM, IgK and Ig lambda). The assays are based on the separation of free from antibody-bound radiolabelled fragments of immunoglobulin by precipitation with 13% polyethylene glycol. The precipitate is harvested and washed on glass fibre filters by means of a commercial cell harvester. Radiolabelled lambda chain from Bence Jones urine was used for lambda chain assay and Fab' gamma from pooled IgG for the kappa assay. Labelled Fab or Fc fragments of IgG, IgA and IgM were used for the class specific assays. Selected commercial antisera were used throughout. The sensitivity limits of the assays performed according to the standard procedure were about 20 ng Ig per ml. The assays have been used for the quantification of circulating immune complexes and both cellular and secreted immunoglobulin produced in cell cultures. The results demonstrate the importance of the simultaneous quantification by L chain and H chain specific assays.

KW - Animals

KW - Antigen-Antibody Complex

KW - Chemical Precipitation

KW - Chickens

KW - Detergents

KW - Humans

KW - Immunoglobulin A

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Immunoglobulin kappa-Chains

KW - Immunoglobulin lambda-Chains

KW - Immunoglobulins

KW - Polyethylene Glycols

KW - Rabbits

KW - Radioimmunoassay

M3 - Journal article

C2 - 6402544

VL - 56

SP - 19

EP - 32

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -