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Jens Christian Jensenius

Primary structure of bovine collectin-43 (CL-43). Comparison with conglutinin and lung surfactant protein-D

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Collectin-43 (CL-43) is a bovine serum protein that is composed of subunits of three identical chains, each of which contains a collagen region and a C-type carbohydrate recognition domain; thus, CL-43 belongs to the collectins (group III of the C-type lectins). We have derived the complete primary sequence of CL-43 using partial protein sequencing, cDNA cloning, and reverse transcription-polymerase chain reaction techniques. The primary sequence of CL-43 shows that it contains an N-terminal region of 28 residues, followed by a collagenous domain of 38 repeats of Gly-Xaa-Yaa and then a C-terminal section of 159 residues, containing a short "neck" region and the carbohydrate recognition domain with the conserved residues found in all C-type lectins. The amino acid sequence of CL-43 showed 74% identity to bovine conglutinin and 70% identity to bovine lung surfactant protein D (SP-D), but the collagen region is considerably shorter than the 57 Gly-Xaa-Yaa triplets found in conglutinin and SP-D. Northern blot analysis showed that CL-43 was only synthesized in bovine liver, with no detectable signal in a variety of other bovine tissues, including lung. No cross-hybridizing signals were detected in mRNA from sheep, human, rat, or mouse liver. Since CL-43 and conglutinin have only been detected in members of bovidae, it is probable that an ancestral gene of these two proteins was first derived from a SP-D-like gene, and that this ancestral gene duplicated during evolution.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume269
Issue16
Pages (from-to)11820-4
Number of pages5
ISSN0021-9258
Publication statusPublished - 1994

    Research areas

  • Amino Acid Sequence, Animals, Base Sequence, Blood Proteins, Cattle, Cloning, Molecular, Collectins, DNA, Complementary, Glycoproteins, Lectins, Liver, Molecular Sequence Data, Oligonucleotide Probes, Polymerase Chain Reaction, Pulmonary Surfactant-Associated Protein D, Pulmonary Surfactants, Recombinant Proteins, Sequence Homology, Amino Acid, Serum Globulins

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