Aarhus University Seal / Aarhus Universitets segl

Jens Christian Jensenius

Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

α-Lactalbumin is a ubiquitous calcium-binding milk protein with a well-characterized function in regulating the synthesis of lactose. An entirely different activity has been shown to occur when a complex is formed between calcium-free α-lactalbumin and oleic acid. This complex shows strong cytotoxic action against several cancer cells, and several mechanisms have been suggested to account for this cell-killing activity. Most studies have been performed using the human protein, but bovine α-lactalbumin shows similar activity. A new and simple 2-step method for purification of calcium-free α-lactalbumin has been developed, and the resulting highly purified preparation was used to generate a complex with oleic acid. Using 3 different cell lines and 2 types of cell viability assays, the bovine and human α-lactalbumin showed comparable cytotoxic activity. The effect was apparent after 15 min of incubation and was inhibited by the presence of fetal bovine serum or bovine serum albumin. The bovine protein might be a useful alternative to the human protein, but also raises the question whether cytotoxic activity could be generated in different kinds of food containing α-lactalbumin.
Original languageEnglish
JournalIndian Journal of Dairy Science
Pages (from-to)2159-2170
Number of pages12
Publication statusPublished - 2011

    Research areas

  • Animals, Cattle, Cell Count, Cell Line, Tumor, Culture Media, Serum-Free, Cytotoxins, HL-60 Cells, Humans, Lactalbumin, Milk, Milk, Human, Oleic Acid, Oleic Acids, Serum, U937 Cells

See relations at Aarhus University Citationformats

ID: 43925305