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Jens Christian Jensenius

Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes

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Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes. / Siersted, Hans Christian; Jensenius, Jens Christian; Svehag, S E; Brandslund, Ivan.

In: Journal of Clinical & Laboratory Immunology, Vol. 12, No. 4, 1983, p. 201-8.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Siersted, HC, Jensenius, JC, Svehag, SE & Brandslund, I 1983, 'Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes', Journal of Clinical & Laboratory Immunology, vol. 12, no. 4, pp. 201-8.

APA

Siersted, H. C., Jensenius, J. C., Svehag, S. E., & Brandslund, I. (1983). Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes. Journal of Clinical & Laboratory Immunology, 12(4), 201-8.

CBE

Siersted HC, Jensenius JC, Svehag SE, Brandslund I. 1983. Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes. Journal of Clinical & Laboratory Immunology. 12(4):201-8.

MLA

Siersted, Hans Christian et al. "Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes". Journal of Clinical & Laboratory Immunology. 1983, 12(4). 201-8.

Vancouver

Siersted HC, Jensenius JC, Svehag SE, Brandslund I. Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes. Journal of Clinical & Laboratory Immunology. 1983;12(4):201-8.

Author

Siersted, Hans Christian ; Jensenius, Jens Christian ; Svehag, S E ; Brandslund, Ivan. / Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes. In: Journal of Clinical & Laboratory Immunology. 1983 ; Vol. 12, No. 4. pp. 201-8.

Bibtex

@article{0ae4b0b3093d42e58ee0d1e0bd0dabdb,
title = "Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes",
abstract = "Techniques for the quantification of C3d are shown to estimate the sum of 4 different plasma protein components possessing C3d but not C3c epitopes. All 4 components were C3-derived polypeptides as shown by activating serum containing 125I-labelled C3, isolating the anti-C3d reactive material in 14% PEG supernatant, followed by analysis on SDS-PAGE and autoradiography. Identical results were obtained by radiolabelling 14% PEG plasma supernatants followed by analysis of the anti-C3d reactive material. The components are referred to as d1, d1', d2 and d3 based on their relative electrophoretic mobilities (alpha 1, alpha 1, alpha 2 and alpha 2 respectively) judged by crossed immunoelectrophoresis. Their apparent molecular weights by SDS-PAGE were 129K (d1), 110K (d1'), 46K (d3) and 45K (d2). The possibility that one or more of the C3d containing components represented a complex of a C3 fragment with another plasma protein was investigated. The role of these components in the scheme of the physiological breakdown of C3 and the importance of the individual C3d components as indicators of complement activation in clinical materials is discussed. It is proposed that the 45K d2 component represents a final physiological breakdown product of C3 in human serum.",
keywords = "Antigen-Antibody Reactions, Chemical Precipitation, Complement C3, Complement C3c, Complement C3d, Electrophoresis, Polyacrylamide Gel, Epitopes, Humans, Immunoelectrophoresis, Two-Dimensional, Molecular Weight",
author = "Siersted, {Hans Christian} and Jensenius, {Jens Christian} and Svehag, {S E} and Ivan Brandslund",
year = "1983",
language = "English",
volume = "12",
pages = "201--8",
journal = "Journal of Clinical & Laboratory Immunology",
issn = "0141-2760",
publisher = "Teviot Scientific Publications Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Partial characterization of physiologically generated C3 components expressing C3d but not C3c epitopes

AU - Siersted, Hans Christian

AU - Jensenius, Jens Christian

AU - Svehag, S E

AU - Brandslund, Ivan

PY - 1983

Y1 - 1983

N2 - Techniques for the quantification of C3d are shown to estimate the sum of 4 different plasma protein components possessing C3d but not C3c epitopes. All 4 components were C3-derived polypeptides as shown by activating serum containing 125I-labelled C3, isolating the anti-C3d reactive material in 14% PEG supernatant, followed by analysis on SDS-PAGE and autoradiography. Identical results were obtained by radiolabelling 14% PEG plasma supernatants followed by analysis of the anti-C3d reactive material. The components are referred to as d1, d1', d2 and d3 based on their relative electrophoretic mobilities (alpha 1, alpha 1, alpha 2 and alpha 2 respectively) judged by crossed immunoelectrophoresis. Their apparent molecular weights by SDS-PAGE were 129K (d1), 110K (d1'), 46K (d3) and 45K (d2). The possibility that one or more of the C3d containing components represented a complex of a C3 fragment with another plasma protein was investigated. The role of these components in the scheme of the physiological breakdown of C3 and the importance of the individual C3d components as indicators of complement activation in clinical materials is discussed. It is proposed that the 45K d2 component represents a final physiological breakdown product of C3 in human serum.

AB - Techniques for the quantification of C3d are shown to estimate the sum of 4 different plasma protein components possessing C3d but not C3c epitopes. All 4 components were C3-derived polypeptides as shown by activating serum containing 125I-labelled C3, isolating the anti-C3d reactive material in 14% PEG supernatant, followed by analysis on SDS-PAGE and autoradiography. Identical results were obtained by radiolabelling 14% PEG plasma supernatants followed by analysis of the anti-C3d reactive material. The components are referred to as d1, d1', d2 and d3 based on their relative electrophoretic mobilities (alpha 1, alpha 1, alpha 2 and alpha 2 respectively) judged by crossed immunoelectrophoresis. Their apparent molecular weights by SDS-PAGE were 129K (d1), 110K (d1'), 46K (d3) and 45K (d2). The possibility that one or more of the C3d containing components represented a complex of a C3 fragment with another plasma protein was investigated. The role of these components in the scheme of the physiological breakdown of C3 and the importance of the individual C3d components as indicators of complement activation in clinical materials is discussed. It is proposed that the 45K d2 component represents a final physiological breakdown product of C3 in human serum.

KW - Antigen-Antibody Reactions

KW - Chemical Precipitation

KW - Complement C3

KW - Complement C3c

KW - Complement C3d

KW - Electrophoresis, Polyacrylamide Gel

KW - Epitopes

KW - Humans

KW - Immunoelectrophoresis, Two-Dimensional

KW - Molecular Weight

M3 - Journal article

C2 - 6198522

VL - 12

SP - 201

EP - 208

JO - Journal of Clinical & Laboratory Immunology

JF - Journal of Clinical & Laboratory Immunology

SN - 0141-2760

IS - 4

ER -