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Measurement of C3 conversion by ELISA estimation of neo-d...
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Jens Christian Jensenius
Measurement of C3 conversion by ELISA estimation of neo-determinants on the C3d moiety
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
- U Holmskov-Nielsen ,
- Jens Christian Jensenius
- Børge Teisner ,
- Karin Erb
An ELISA assay estimating neo-determinants on the C3d moiety is described. The C3d neo-determinants were detected by incubating the sample on F(ab')2 anti-C3d-coated plates followed by development with biotin-labelled anti-C3d and enzyme-labelled avidin. The assay was compared with rocket immunoelectrophoresis (IE) and crossed IE. Serum from a factor I deficient patient showed no detectable C3d when analysed by rocket IE, but activation was evident when analysing by crossed IE or ELISA. The results suggest that the neo-determinants detected by ELISA become exposed when C3 is split into C3a and C3b and this interpretation was supported by kinetic analysis of C3 activation by the three methods.
| Original language | English |
|---|---|
| Journal | Journal of Immunological Methods |
| Volume | 94 |
| Issue | 1-2 |
| Pages (from-to) | 1-6 |
| Number of pages | 6 |
| ISSN | 0022-1759 |
| Publication status | Published - 1986 |
- Complement Activation, Complement C3, Complement C3c, Complement C3d, Enzyme-Linked Immunosorbent Assay, Epitopes, Humans, Kinetics
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