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Jens Christian Jensenius

Measurement of C3 conversion by ELISA estimation of neo-determinants on the C3d moiety

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An ELISA assay estimating neo-determinants on the C3d moiety is described. The C3d neo-determinants were detected by incubating the sample on F(ab')2 anti-C3d-coated plates followed by development with biotin-labelled anti-C3d and enzyme-labelled avidin. The assay was compared with rocket immunoelectrophoresis (IE) and crossed IE. Serum from a factor I deficient patient showed no detectable C3d when analysed by rocket IE, but activation was evident when analysing by crossed IE or ELISA. The results suggest that the neo-determinants detected by ELISA become exposed when C3 is split into C3a and C3b and this interpretation was supported by kinetic analysis of C3 activation by the three methods.
Original languageEnglish
JournalJournal of Immunological Methods
Volume94
Issue1-2
Pages (from-to)1-6
Number of pages6
ISSN0022-1759
Publication statusPublished - 1986

    Research areas

  • Complement Activation, Complement C3, Complement C3c, Complement C3d, Enzyme-Linked Immunosorbent Assay, Epitopes, Humans, Kinetics

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