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Jens Christian Jensenius

L-MBP is expressed in epithelial cells of mouse small intestine

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The mannan-binding proteins (L-MBP and S-MBP, also denoted MBL-C and MBL-A), mainly produced in liver and existing in liver and serum, play important roles in the innate immunity against a variety of pathogens. Total RNA from mouse tissues were screened for MBP mRNA by RT-PCR. In addition to liver, S-MBP mRNA was detected in lung, kidney, and testis, and L-MBP mRNA was detected in kidney, thymus, and small intestine. Quantitative RT-PCR revealed that the small intestine is a predominant site of extrahepatic expression of L-MBP. Western blotting with polyclonal Abs against rat L-MBP demonstrated this protein in Triton X-100 extracts of the small intestine obtained from mice that had undergone systemic perfusion. Immunohistochemical staining with an mAb against mouse L-MBP and in situ hybridization revealed that L-MBP is selectively expressed in some villous epithelial cells of the small intestine. These findings suggest that L-MBP plays a role in mucosal innate immunity.
Original languageEnglish
JournalJournal of Immunology
Volume169
Issue12
Pages (from-to)6945-50
Number of pages6
ISSN0022-1767
Publication statusPublished - 2002

    Research areas

  • Animals, Hepatocytes, Immunohistochemistry, In Situ Hybridization, Intestinal Mucosa, Intestine, Small, Kidney, Liver, Lung, Male, Mannose-Binding Lectin, Mice, Mice, Inbred C57BL, Protein Isoforms, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Testis

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