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Jens Christian Jensenius

Interaction of C1q and mannan-binding lectin (MBL) with C1r, C1s, MBL-associated serine proteases 1 and 2, and the MBL-associated protein MAp19.

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Interaction of C1q and mannan-binding lectin (MBL) with C1r, C1s, MBL-associated serine proteases 1 and 2, and the MBL-associated protein MAp19. / Thiel, S; Petersen, Steen Vang; Vorup-Jensen, T; Matsushita, M; Fujita, T; Stover, C M; Schwaeble, W J; Jensenius, J C.

In: Journal of Immunology, Vol. 165, No. 2, 2000, p. 878-87.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{0fc75930fbd911dcb919000ea68e967b,
title = "Interaction of C1q and mannan-binding lectin (MBL) with C1r, C1s, MBL-associated serine proteases 1 and 2, and the MBL-associated protein MAp19.",
abstract = "Mannan-binding lectin (MBL) and C1q activate the complement cascade via attached serine proteases. The proteases C1r and C1s were initially discovered in a complex with C1q, whereas the MBL-associated serine proteases 1 and 2 (MASP-1 and -2) were discovered in a complex with MBL. There is controversy as to whether MBL can utilize C1r and C1s or, inversely, whether C1q can utilize MASP-1 and 2. Serum deficient in C1r produced no complement activation in IgG-coated microwells, whereas activation was seen in mannan-coated microwells. In serum, C1r and C1s were found to be associated only with C1q, whereas MASP-1, MASP-2, and a third protein, MAp19 (19-kDa MBL-associated protein), were found to be associated only with MBL. The bulk of MASP-1 and MAp19 was found in association with each other and was not bound to MBL or MASP-2. The interactions of MASP-1, MASP-2, and MAp19 with MBL differ from those of C1r and C1s with C1q in that both high salt concentrations and calcium chelation (EDTA) are required to fully dissociate the MASPs or MAp19 from MBL. In the presence of calcium, most of the MASP-1, MASP-2, and MAp19 emerged on gel-permeation chromatography as large complexes that were not associated with MBL, whereas in the presence of EDTA most of these components formed smaller complexes. Over 95% of the total MASPs and MAp19 found in serum are not complexed with MBL. Udgivelsesdato: 2000-Jul-15",
keywords = "Calcium, Carrier Proteins, Centrifugation, Density Gradient, Chromatography, Gel, Collectins, Complement C1, Complement C1q, Complement C1r, Complement C1s, Complement C4b, Edetic Acid, Humans, Immune Sera, Immunoglobulin G, Lectins, Mannans, Mannose-Binding Protein-Associated Serine Proteases, Osmolar Concentration, Protein Binding, Serine Endopeptidases",
author = "S Thiel and Petersen, {Steen Vang} and T Vorup-Jensen and M Matsushita and T Fujita and Stover, {C M} and Schwaeble, {W J} and Jensenius, {J C}",
year = "2000",
language = "English",
volume = "165",
pages = "878--87",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

RIS

TY - JOUR

T1 - Interaction of C1q and mannan-binding lectin (MBL) with C1r, C1s, MBL-associated serine proteases 1 and 2, and the MBL-associated protein MAp19.

AU - Thiel, S

AU - Petersen, Steen Vang

AU - Vorup-Jensen, T

AU - Matsushita, M

AU - Fujita, T

AU - Stover, C M

AU - Schwaeble, W J

AU - Jensenius, J C

PY - 2000

Y1 - 2000

N2 - Mannan-binding lectin (MBL) and C1q activate the complement cascade via attached serine proteases. The proteases C1r and C1s were initially discovered in a complex with C1q, whereas the MBL-associated serine proteases 1 and 2 (MASP-1 and -2) were discovered in a complex with MBL. There is controversy as to whether MBL can utilize C1r and C1s or, inversely, whether C1q can utilize MASP-1 and 2. Serum deficient in C1r produced no complement activation in IgG-coated microwells, whereas activation was seen in mannan-coated microwells. In serum, C1r and C1s were found to be associated only with C1q, whereas MASP-1, MASP-2, and a third protein, MAp19 (19-kDa MBL-associated protein), were found to be associated only with MBL. The bulk of MASP-1 and MAp19 was found in association with each other and was not bound to MBL or MASP-2. The interactions of MASP-1, MASP-2, and MAp19 with MBL differ from those of C1r and C1s with C1q in that both high salt concentrations and calcium chelation (EDTA) are required to fully dissociate the MASPs or MAp19 from MBL. In the presence of calcium, most of the MASP-1, MASP-2, and MAp19 emerged on gel-permeation chromatography as large complexes that were not associated with MBL, whereas in the presence of EDTA most of these components formed smaller complexes. Over 95% of the total MASPs and MAp19 found in serum are not complexed with MBL. Udgivelsesdato: 2000-Jul-15

AB - Mannan-binding lectin (MBL) and C1q activate the complement cascade via attached serine proteases. The proteases C1r and C1s were initially discovered in a complex with C1q, whereas the MBL-associated serine proteases 1 and 2 (MASP-1 and -2) were discovered in a complex with MBL. There is controversy as to whether MBL can utilize C1r and C1s or, inversely, whether C1q can utilize MASP-1 and 2. Serum deficient in C1r produced no complement activation in IgG-coated microwells, whereas activation was seen in mannan-coated microwells. In serum, C1r and C1s were found to be associated only with C1q, whereas MASP-1, MASP-2, and a third protein, MAp19 (19-kDa MBL-associated protein), were found to be associated only with MBL. The bulk of MASP-1 and MAp19 was found in association with each other and was not bound to MBL or MASP-2. The interactions of MASP-1, MASP-2, and MAp19 with MBL differ from those of C1r and C1s with C1q in that both high salt concentrations and calcium chelation (EDTA) are required to fully dissociate the MASPs or MAp19 from MBL. In the presence of calcium, most of the MASP-1, MASP-2, and MAp19 emerged on gel-permeation chromatography as large complexes that were not associated with MBL, whereas in the presence of EDTA most of these components formed smaller complexes. Over 95% of the total MASPs and MAp19 found in serum are not complexed with MBL. Udgivelsesdato: 2000-Jul-15

KW - Calcium

KW - Carrier Proteins

KW - Centrifugation, Density Gradient

KW - Chromatography, Gel

KW - Collectins

KW - Complement C1

KW - Complement C1q

KW - Complement C1r

KW - Complement C1s

KW - Complement C4b

KW - Edetic Acid

KW - Humans

KW - Immune Sera

KW - Immunoglobulin G

KW - Lectins

KW - Mannans

KW - Mannose-Binding Protein-Associated Serine Proteases

KW - Osmolar Concentration

KW - Protein Binding

KW - Serine Endopeptidases

M3 - Journal article

C2 - 10878362

VL - 165

SP - 878

EP - 887

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -