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Jens Christian Jensenius

Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1

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Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1. / Ditzel, H; Rasmussen, J W; Borup-Christensen, P; Erb, Karin; Jensenius, Jens Christian.

In: Cancer, Vol. 73, No. 3 Suppl, 1994, p. 858-63.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Ditzel, H, Rasmussen, JW, Borup-Christensen, P, Erb, K & Jensenius, JC 1994, 'Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1', Cancer, vol. 73, no. 3 Suppl, pp. 858-63.

APA

Ditzel, H., Rasmussen, J. W., Borup-Christensen, P., Erb, K., & Jensenius, J. C. (1994). Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1. Cancer, 73(3 Suppl), 858-63.

CBE

Ditzel H, Rasmussen JW, Borup-Christensen P, Erb K, Jensenius JC. 1994. Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1. Cancer. 73(3 Suppl):858-63.

MLA

Ditzel, H et al. "Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1". Cancer. 1994, 73(3 Suppl). 858-63.

Vancouver

Ditzel H, Rasmussen JW, Borup-Christensen P, Erb K, Jensenius JC. Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1. Cancer. 1994;73(3 Suppl):858-63.

Author

Ditzel, H ; Rasmussen, J W ; Borup-Christensen, P ; Erb, Karin ; Jensenius, Jens Christian. / Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1. In: Cancer. 1994 ; Vol. 73, No. 3 Suppl. pp. 858-63.

Bibtex

@article{2072db5f739c4359865eb6d25678ea36,
title = "Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1",
abstract = "The human monoclonal immunoglobulin M (IgM) antibody, COU-1 is obtained from a human-human hybridoma, which is derived by fusion between a human B-lymphoblastoid cell line and lymphocytes obtained from mesenteric lymph nodes from a patient with colorectal cancer. COU-1 recognizes a 43 kilodaltons intracellularly located cytokeratin-like protein, strongly expressed by adenocarcinoma tissue as compared to normal tissues. In tumor-bearing nude mice, antibody COU-1 labeled with 125I has been shown to accumulate in human colon cancer grafts when compared to human melanoma grafts and the normal mouse tissues. The observed accumulation was sufficient to be detected externally by immunoscintigraphy. Antigen-binding fragments of the antibody were also prepared and were shown to accumulate in colon cancer grafts. Improved tumor to normal tissue ratio was seen with the half-monomeric fragment, and the time required was reduced. In the clinic, five patients with suspected colorectal cancer were given 2 mg of 131I-labeled COU-1. No adverse effects were detected in any of the patients. Planar images were obtained on days 1, 2, 3, 5, and 7 after administration. The best images were obtained on days 5 and 7. Tumors were localized by immunoscintigraphy in four of the patients. Of these patients, surgery revealed that three of them had primary colorectal cancers located in the cecum, the ascending colon, and the rectum, respectively, while one patient had a pancreatic cancer. The smallest lesion observed had a diameter of 3 cm. In one of the patients, otherwise undiagnosed multiple liver metastases were revealed by the immunoscintigraphy and confirmed at surgery. An x-ray of the colon performed on the fifth patient had shown a stricture in the descending colon suspected to be caused by cancer. The tumor scintigraphy showed no accumulation of the antibody. Surgery revealed that the stricture was caused by adherence and not cancer. These findings are encouraging for further studies of this human monoclonal antibody in cancer patients.",
keywords = "Adenocarcinoma, Antibodies, Monoclonal, Colonic Neoplasms, Colorectal Neoplasms, Humans, Immunoglobulin Fragments, Immunoglobulin M, Iodine Radioisotopes, Radioimmunodetection, Time Factors",
author = "H Ditzel and Rasmussen, {J W} and P Borup-Christensen and Karin Erb and Jensenius, {Jens Christian}",
year = "1994",
language = "English",
volume = "73",
pages = "858--63",
journal = "Cancer",
issn = "0008-543X",
publisher = "JohnWiley & Sons, Inc.",
number = "3 Suppl",

}

RIS

TY - JOUR

T1 - Immunoscintigraphy of colon cancers with the human monoclonal antibody COU-1

AU - Ditzel, H

AU - Rasmussen, J W

AU - Borup-Christensen, P

AU - Erb, Karin

AU - Jensenius, Jens Christian

PY - 1994

Y1 - 1994

N2 - The human monoclonal immunoglobulin M (IgM) antibody, COU-1 is obtained from a human-human hybridoma, which is derived by fusion between a human B-lymphoblastoid cell line and lymphocytes obtained from mesenteric lymph nodes from a patient with colorectal cancer. COU-1 recognizes a 43 kilodaltons intracellularly located cytokeratin-like protein, strongly expressed by adenocarcinoma tissue as compared to normal tissues. In tumor-bearing nude mice, antibody COU-1 labeled with 125I has been shown to accumulate in human colon cancer grafts when compared to human melanoma grafts and the normal mouse tissues. The observed accumulation was sufficient to be detected externally by immunoscintigraphy. Antigen-binding fragments of the antibody were also prepared and were shown to accumulate in colon cancer grafts. Improved tumor to normal tissue ratio was seen with the half-monomeric fragment, and the time required was reduced. In the clinic, five patients with suspected colorectal cancer were given 2 mg of 131I-labeled COU-1. No adverse effects were detected in any of the patients. Planar images were obtained on days 1, 2, 3, 5, and 7 after administration. The best images were obtained on days 5 and 7. Tumors were localized by immunoscintigraphy in four of the patients. Of these patients, surgery revealed that three of them had primary colorectal cancers located in the cecum, the ascending colon, and the rectum, respectively, while one patient had a pancreatic cancer. The smallest lesion observed had a diameter of 3 cm. In one of the patients, otherwise undiagnosed multiple liver metastases were revealed by the immunoscintigraphy and confirmed at surgery. An x-ray of the colon performed on the fifth patient had shown a stricture in the descending colon suspected to be caused by cancer. The tumor scintigraphy showed no accumulation of the antibody. Surgery revealed that the stricture was caused by adherence and not cancer. These findings are encouraging for further studies of this human monoclonal antibody in cancer patients.

AB - The human monoclonal immunoglobulin M (IgM) antibody, COU-1 is obtained from a human-human hybridoma, which is derived by fusion between a human B-lymphoblastoid cell line and lymphocytes obtained from mesenteric lymph nodes from a patient with colorectal cancer. COU-1 recognizes a 43 kilodaltons intracellularly located cytokeratin-like protein, strongly expressed by adenocarcinoma tissue as compared to normal tissues. In tumor-bearing nude mice, antibody COU-1 labeled with 125I has been shown to accumulate in human colon cancer grafts when compared to human melanoma grafts and the normal mouse tissues. The observed accumulation was sufficient to be detected externally by immunoscintigraphy. Antigen-binding fragments of the antibody were also prepared and were shown to accumulate in colon cancer grafts. Improved tumor to normal tissue ratio was seen with the half-monomeric fragment, and the time required was reduced. In the clinic, five patients with suspected colorectal cancer were given 2 mg of 131I-labeled COU-1. No adverse effects were detected in any of the patients. Planar images were obtained on days 1, 2, 3, 5, and 7 after administration. The best images were obtained on days 5 and 7. Tumors were localized by immunoscintigraphy in four of the patients. Of these patients, surgery revealed that three of them had primary colorectal cancers located in the cecum, the ascending colon, and the rectum, respectively, while one patient had a pancreatic cancer. The smallest lesion observed had a diameter of 3 cm. In one of the patients, otherwise undiagnosed multiple liver metastases were revealed by the immunoscintigraphy and confirmed at surgery. An x-ray of the colon performed on the fifth patient had shown a stricture in the descending colon suspected to be caused by cancer. The tumor scintigraphy showed no accumulation of the antibody. Surgery revealed that the stricture was caused by adherence and not cancer. These findings are encouraging for further studies of this human monoclonal antibody in cancer patients.

KW - Adenocarcinoma

KW - Antibodies, Monoclonal

KW - Colonic Neoplasms

KW - Colorectal Neoplasms

KW - Humans

KW - Immunoglobulin Fragments

KW - Immunoglobulin M

KW - Iodine Radioisotopes

KW - Radioimmunodetection

KW - Time Factors

M3 - Journal article

C2 - 8306271

VL - 73

SP - 858

EP - 863

JO - Cancer

JF - Cancer

SN - 0008-543X

IS - 3 Suppl

ER -