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Jens Christian Jensenius

Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia

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Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia. / Ditzel, H; Erb, Karin; Teisner, Børge; Nielsen, B; Borup-Christensen, P; Jensenius, Jens Christian.

In: Histochemistry, Vol. 94, No. 1, 1990, p. 95-9.

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Ditzel, H ; Erb, Karin ; Teisner, Børge ; Nielsen, B ; Borup-Christensen, P ; Jensenius, Jens Christian. / Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia. In: Histochemistry. 1990 ; Vol. 94, No. 1. pp. 95-9.

Bibtex

@article{446ce85fbb6840188ec63e19e7db95c8,
title = "Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia",
abstract = "We have analysed the binding of human IgM to fetal, normal adult and malignant colo-rectal tissues. Using an indirect immunoperoxidase technique on sections of frozen tissues human IgM binds to all normal adult colo-rectal epithelia (n = 15) tested. By contrast, 9 out of 25 colo-rectal adenocarcinomas were negative, and in the remaining 16 the staining reaction varied from staining of all the cancer areas to focal staining of a few areas. Human IgM did not bind to 14 samples of fetal intestinal epithelium (gestational age of 6-14 weeks). The binding of IgM was found to be mediated by secretory component (SC) as anti-SC antibody (anti-SC) showed a similar staining pattern as IgM and the IgM binding could be blocked by anti-SC. SC was also demonstrated in glandular epithelia of sections of all normal breast epithelia but only in 10 out of 15 breast adenocarcinomas. The loss of IgM binding and SC could not be correlated to the morphology of the adenocarcinomas. The observations on fetal, normal adult and malignant tissue suggest that IgM binding and SC may be gradually lost during dedifferentiation of normal cells, to malignant colo-rectal or breast epithelia.",
keywords = "Adenocarcinoma, Adult, Breast, Breast Neoplasms, Colon, Colonic Neoplasms, Digestive System, Epithelium, Fetus, Humans, Immunoglobulin Fragments, Immunoglobulin M, Secretory Component, Waldenstrom Macroglobulinemia",
author = "H Ditzel and Karin Erb and B{\o}rge Teisner and B Nielsen and P Borup-Christensen and Jensenius, {Jens Christian}",
year = "1990",
language = "English",
volume = "94",
pages = "95--9",
journal = "Histochemistry",
issn = "0301-5564",
publisher = "Springer Verlag",
number = "1",

}

RIS

TY - JOUR

T1 - Immunohistochemical analysis of the binding of human IgM to secretory component present in normal adult colon epithelia, but not in colon cancer and fetal colon epithelia

AU - Ditzel, H

AU - Erb, Karin

AU - Teisner, Børge

AU - Nielsen, B

AU - Borup-Christensen, P

AU - Jensenius, Jens Christian

PY - 1990

Y1 - 1990

N2 - We have analysed the binding of human IgM to fetal, normal adult and malignant colo-rectal tissues. Using an indirect immunoperoxidase technique on sections of frozen tissues human IgM binds to all normal adult colo-rectal epithelia (n = 15) tested. By contrast, 9 out of 25 colo-rectal adenocarcinomas were negative, and in the remaining 16 the staining reaction varied from staining of all the cancer areas to focal staining of a few areas. Human IgM did not bind to 14 samples of fetal intestinal epithelium (gestational age of 6-14 weeks). The binding of IgM was found to be mediated by secretory component (SC) as anti-SC antibody (anti-SC) showed a similar staining pattern as IgM and the IgM binding could be blocked by anti-SC. SC was also demonstrated in glandular epithelia of sections of all normal breast epithelia but only in 10 out of 15 breast adenocarcinomas. The loss of IgM binding and SC could not be correlated to the morphology of the adenocarcinomas. The observations on fetal, normal adult and malignant tissue suggest that IgM binding and SC may be gradually lost during dedifferentiation of normal cells, to malignant colo-rectal or breast epithelia.

AB - We have analysed the binding of human IgM to fetal, normal adult and malignant colo-rectal tissues. Using an indirect immunoperoxidase technique on sections of frozen tissues human IgM binds to all normal adult colo-rectal epithelia (n = 15) tested. By contrast, 9 out of 25 colo-rectal adenocarcinomas were negative, and in the remaining 16 the staining reaction varied from staining of all the cancer areas to focal staining of a few areas. Human IgM did not bind to 14 samples of fetal intestinal epithelium (gestational age of 6-14 weeks). The binding of IgM was found to be mediated by secretory component (SC) as anti-SC antibody (anti-SC) showed a similar staining pattern as IgM and the IgM binding could be blocked by anti-SC. SC was also demonstrated in glandular epithelia of sections of all normal breast epithelia but only in 10 out of 15 breast adenocarcinomas. The loss of IgM binding and SC could not be correlated to the morphology of the adenocarcinomas. The observations on fetal, normal adult and malignant tissue suggest that IgM binding and SC may be gradually lost during dedifferentiation of normal cells, to malignant colo-rectal or breast epithelia.

KW - Adenocarcinoma

KW - Adult

KW - Breast

KW - Breast Neoplasms

KW - Colon

KW - Colonic Neoplasms

KW - Digestive System

KW - Epithelium

KW - Fetus

KW - Humans

KW - Immunoglobulin Fragments

KW - Immunoglobulin M

KW - Secretory Component

KW - Waldenstrom Macroglobulinemia

M3 - Journal article

C2 - 2112519

VL - 94

SP - 95

EP - 99

JO - Histochemistry

JF - Histochemistry

SN - 0301-5564

IS - 1

ER -