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Jens Christian Jensenius

ELISA quantitation of IgG subclass antibodies to dietary antigens

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The IgG subclasses of human antibodies against 2 dietary antigens, ovalbumin (OA) and beta-lactoglobulin (BLG), were studied by ELISA using monoclonal anti-human IgG subclass antibodies. Under the assay conditions used, the anti-IgG subclass antibodies were subclass specific. Quantitative estimates of the subclass antibodies were obtained by reference to a 'capture' assay using F(ab')2 anti-light chain antibody as ligand and IgG myelomas as standards. The validity of these estimates was supported by antibody quantitation using the Farr assay. In healthy adults with serum anti-OA or anti-BLG antibodies, anti-OA antibodies were found mainly in the IgG1 (9/11) and IgG4 (6/11) subclasses, whereas 5 sera showed high levels of IgG2 antibodies. In contrast, the IgG subclass distribution of anti-BLG antibodies was predominantly IgG4 (10/10).
Original languageEnglish
JournalJournal of Immunological Methods
Volume82
Issue2
Pages (from-to)321-31
Number of pages11
ISSN0022-1759
Publication statusPublished - 1985

    Research areas

  • Adult, Antibodies, Anti-Idiotypic, Antibodies, Monoclonal, Antibody Specificity, Diet, Enzyme-Linked Immunosorbent Assay, Female, Food Hypersensitivity, Humans, Immunoglobulin G, Lactoglobulins, Male, Myeloma Proteins, Ovalbumin

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