Jens Christian Jensenius

Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production

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Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production. / White, Mitchell R; Tripathi, Shweta; Verma, Anamika; Kingma, Paul; Takahashi, Kazue; Jensenius, Jens; Thiel, Steffen; Wang, Guangshun; Crouch, Erika C; Hartshorn, Kevan L.

In: Innate Immunity, Vol. 23, No. 1, 01.2017, p. 77-88.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

White, MR, Tripathi, S, Verma, A, Kingma, P, Takahashi, K, Jensenius, J, Thiel, S, Wang, G, Crouch, EC & Hartshorn, KL 2017, 'Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production', Innate Immunity, vol. 23, no. 1, pp. 77-88. https://doi.org/10.1177/1753425916678470

APA

White, M. R., Tripathi, S., Verma, A., Kingma, P., Takahashi, K., Jensenius, J., ... Hartshorn, K. L. (2017). Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production. Innate Immunity, 23(1), 77-88. https://doi.org/10.1177/1753425916678470

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Author

White, Mitchell R ; Tripathi, Shweta ; Verma, Anamika ; Kingma, Paul ; Takahashi, Kazue ; Jensenius, Jens ; Thiel, Steffen ; Wang, Guangshun ; Crouch, Erika C ; Hartshorn, Kevan L. / Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production. In: Innate Immunity. 2017 ; Vol. 23, No. 1. pp. 77-88.

Bibtex

@article{c2979de3b0734241b886d2f9c0ce7ebe,
title = "Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production",
abstract = "Infiltrating activated monocytes are important mediators of damaging inflammation during influenza A virus (IAV) infection. We show that soluble respiratory proteins [collectins, surfactant proteins D (SP-D) and mannose binding lectin (MBL), H-ficolin and LL-37] inhibit replication of seasonal IAV in human monocytes. The collectins and H-ficolin also increased viral uptake by the cells, while LL-37 did not. H-ficolin was able to inhibit replication of the 2009 pandemic H1N1 strain (Cal09) in monocytes, but SP-D and LL-37 had significantly fewer inhibitory effects on this strain than on seasonal IAV. All of these proteins reduced IAV-induced TNF-α production, even in instances when viral replication was not reduced. We used modified recombinant versions of SP-D, MBL and ficolin to elucidate mechanisms through which these proteins alter monocyte interactions with IAV. We demonstrate the importance of the multimeric structure, and of binding properties of the lectin domain, in mediating antiviral and opsonic activity of the proteins. Hence, soluble inhibitors present in airway lining fluid may aid clearance of IAV by promoting monocyte uptake of the virus, while reducing viral replication and virus-induced TNF-α responses in these cells. However, SP-D and LL-37 have reduced ability to inhibit replication of pandemic IAV in monocytes.",
author = "White, {Mitchell R} and Shweta Tripathi and Anamika Verma and Paul Kingma and Kazue Takahashi and Jens Jensenius and Steffen Thiel and Guangshun Wang and Crouch, {Erika C} and Hartshorn, {Kevan L}",
year = "2017",
month = "1",
doi = "10.1177/1753425916678470",
language = "English",
volume = "23",
pages = "77--88",
journal = "Innate Immunity",
issn = "1753-4259",
publisher = "SAGE Publications",
number = "1",

}

RIS

TY - JOUR

T1 - Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production

AU - White, Mitchell R

AU - Tripathi, Shweta

AU - Verma, Anamika

AU - Kingma, Paul

AU - Takahashi, Kazue

AU - Jensenius, Jens

AU - Thiel, Steffen

AU - Wang, Guangshun

AU - Crouch, Erika C

AU - Hartshorn, Kevan L

PY - 2017/1

Y1 - 2017/1

N2 - Infiltrating activated monocytes are important mediators of damaging inflammation during influenza A virus (IAV) infection. We show that soluble respiratory proteins [collectins, surfactant proteins D (SP-D) and mannose binding lectin (MBL), H-ficolin and LL-37] inhibit replication of seasonal IAV in human monocytes. The collectins and H-ficolin also increased viral uptake by the cells, while LL-37 did not. H-ficolin was able to inhibit replication of the 2009 pandemic H1N1 strain (Cal09) in monocytes, but SP-D and LL-37 had significantly fewer inhibitory effects on this strain than on seasonal IAV. All of these proteins reduced IAV-induced TNF-α production, even in instances when viral replication was not reduced. We used modified recombinant versions of SP-D, MBL and ficolin to elucidate mechanisms through which these proteins alter monocyte interactions with IAV. We demonstrate the importance of the multimeric structure, and of binding properties of the lectin domain, in mediating antiviral and opsonic activity of the proteins. Hence, soluble inhibitors present in airway lining fluid may aid clearance of IAV by promoting monocyte uptake of the virus, while reducing viral replication and virus-induced TNF-α responses in these cells. However, SP-D and LL-37 have reduced ability to inhibit replication of pandemic IAV in monocytes.

AB - Infiltrating activated monocytes are important mediators of damaging inflammation during influenza A virus (IAV) infection. We show that soluble respiratory proteins [collectins, surfactant proteins D (SP-D) and mannose binding lectin (MBL), H-ficolin and LL-37] inhibit replication of seasonal IAV in human monocytes. The collectins and H-ficolin also increased viral uptake by the cells, while LL-37 did not. H-ficolin was able to inhibit replication of the 2009 pandemic H1N1 strain (Cal09) in monocytes, but SP-D and LL-37 had significantly fewer inhibitory effects on this strain than on seasonal IAV. All of these proteins reduced IAV-induced TNF-α production, even in instances when viral replication was not reduced. We used modified recombinant versions of SP-D, MBL and ficolin to elucidate mechanisms through which these proteins alter monocyte interactions with IAV. We demonstrate the importance of the multimeric structure, and of binding properties of the lectin domain, in mediating antiviral and opsonic activity of the proteins. Hence, soluble inhibitors present in airway lining fluid may aid clearance of IAV by promoting monocyte uptake of the virus, while reducing viral replication and virus-induced TNF-α responses in these cells. However, SP-D and LL-37 have reduced ability to inhibit replication of pandemic IAV in monocytes.

U2 - 10.1177/1753425916678470

DO - 10.1177/1753425916678470

M3 - Journal article

VL - 23

SP - 77

EP - 88

JO - Innate Immunity

JF - Innate Immunity

SN - 1753-4259

IS - 1

ER -