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Jens Christian Jensenius

Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen

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Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen. / Erb, Karin; Borup-Christensen, P; Ditzel, H; Chemnitz, John; Haas, Henning de; Jensenius, Jens Christian.

In: Hybridoma, Vol. 11, No. 2, 1992, p. 121-34.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Erb, K, Borup-Christensen, P, Ditzel, H, Chemnitz, J, Haas, HD & Jensenius, JC 1992, 'Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen', Hybridoma, vol. 11, no. 2, pp. 121-34.

APA

Erb, K., Borup-Christensen, P., Ditzel, H., Chemnitz, J., Haas, H. D., & Jensenius, J. C. (1992). Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen. Hybridoma, 11(2), 121-34.

CBE

Erb K, Borup-Christensen P, Ditzel H, Chemnitz J, Haas HD, Jensenius JC. 1992. Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen. Hybridoma. 11(2):121-34.

MLA

Vancouver

Erb K, Borup-Christensen P, Ditzel H, Chemnitz J, Haas HD, Jensenius JC. Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen. Hybridoma. 1992;11(2):121-34.

Author

Erb, Karin ; Borup-Christensen, P ; Ditzel, H ; Chemnitz, John ; Haas, Henning de ; Jensenius, Jens Christian. / Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen. In: Hybridoma. 1992 ; Vol. 11, No. 2. pp. 121-34.

Bibtex

@article{6f5840e06b754a71a856014c6b095c62,
title = "Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen",
abstract = "The human hybridoma cell line, B9165, was obtained after fusion of lymphocytes from lymph nodes draining the tumor region in a patient with adenocarcinoma of the colon with the human B-lymphoblastoid cell line WI-L2-729-HF2 (729-HF2). B9165 secretes the human monoclonal antibody, C-OU1 (IgM, kappa). Immunocytochemical and immunohistochemical analysis showed that the antibody bound to a differentiation antigen. Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells. No reaction was seen with intermediate filaments in normal colonic epithelium. The molecular weight of the antigen was shown to be 43 Kda by SDS-PAGE and Western blotting of tumor extracts, and isoelectric focusing of sonicated extracts demonstrated reaction with molecular species of pI 5.4-6.2. These findings suggest that the C-OU1 antigen is a modified cytokeratin 18. The B9165 cell line has proved to be quite stable, and the antibody is of potential clinical value. Its usefulness for localizing tumors in patients is being investigated.",
keywords = "Antibodies, Monoclonal, Antibodies, Neoplasm, Antigens, Differentiation, Antigens, Neoplasm, Colonic Neoplasms, Humans, Hybridomas, Immunohistochemistry, Intermediate Filaments, Microscopy, Electron",
author = "Karin Erb and P Borup-Christensen and H Ditzel and John Chemnitz and Haas, {Henning de} and Jensenius, {Jens Christian}",
year = "1992",
language = "English",
volume = "11",
pages = "121--34",
journal = "Hybridoma",
issn = "1554-0014",
publisher = "Mary Ann/Liebert, Inc. Publishers",
number = "2",

}

RIS

TY - JOUR

T1 - Characterization of a human-human hybridoma antibody, C-OU1, directed against a colon tumor-associated antigen

AU - Erb, Karin

AU - Borup-Christensen, P

AU - Ditzel, H

AU - Chemnitz, John

AU - Haas, Henning de

AU - Jensenius, Jens Christian

PY - 1992

Y1 - 1992

N2 - The human hybridoma cell line, B9165, was obtained after fusion of lymphocytes from lymph nodes draining the tumor region in a patient with adenocarcinoma of the colon with the human B-lymphoblastoid cell line WI-L2-729-HF2 (729-HF2). B9165 secretes the human monoclonal antibody, C-OU1 (IgM, kappa). Immunocytochemical and immunohistochemical analysis showed that the antibody bound to a differentiation antigen. Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells. No reaction was seen with intermediate filaments in normal colonic epithelium. The molecular weight of the antigen was shown to be 43 Kda by SDS-PAGE and Western blotting of tumor extracts, and isoelectric focusing of sonicated extracts demonstrated reaction with molecular species of pI 5.4-6.2. These findings suggest that the C-OU1 antigen is a modified cytokeratin 18. The B9165 cell line has proved to be quite stable, and the antibody is of potential clinical value. Its usefulness for localizing tumors in patients is being investigated.

AB - The human hybridoma cell line, B9165, was obtained after fusion of lymphocytes from lymph nodes draining the tumor region in a patient with adenocarcinoma of the colon with the human B-lymphoblastoid cell line WI-L2-729-HF2 (729-HF2). B9165 secretes the human monoclonal antibody, C-OU1 (IgM, kappa). Immunocytochemical and immunohistochemical analysis showed that the antibody bound to a differentiation antigen. Electron microscopy of colonic adenocarcinoma cells, intact tumor and colonic epithelium by the immunogold technique demonstrated that the C-OU1 antibody reacted with a molecule associated with areas of disruption of the intermediate filaments in the cytoplasm of the tumor cells. No reaction was seen with intermediate filaments in normal colonic epithelium. The molecular weight of the antigen was shown to be 43 Kda by SDS-PAGE and Western blotting of tumor extracts, and isoelectric focusing of sonicated extracts demonstrated reaction with molecular species of pI 5.4-6.2. These findings suggest that the C-OU1 antigen is a modified cytokeratin 18. The B9165 cell line has proved to be quite stable, and the antibody is of potential clinical value. Its usefulness for localizing tumors in patients is being investigated.

KW - Antibodies, Monoclonal

KW - Antibodies, Neoplasm

KW - Antigens, Differentiation

KW - Antigens, Neoplasm

KW - Colonic Neoplasms

KW - Humans

KW - Hybridomas

KW - Immunohistochemistry

KW - Intermediate Filaments

KW - Microscopy, Electron

M3 - Journal article

C2 - 1607210

VL - 11

SP - 121

EP - 134

JO - Hybridoma

JF - Hybridoma

SN - 1554-0014

IS - 2

ER -