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Jens Christian Jensenius

Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. / Liu, H; Jensen, L; Hansen, S; Petersen, Steen Vang; Takahashi, K; Ezekowitz, A B; Dagnæs-Hansen, Frederik; Jensenius, J C; Thiel, S.

In: Scandinavian Journal of Immunology, Vol. 53, No. 5, 2001, p. 489-97.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Liu, H, Jensen, L, Hansen, S, Petersen, SV, Takahashi, K, Ezekowitz, AB, Dagnæs-Hansen, F, Jensenius, JC & Thiel, S 2001, 'Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses.', Scandinavian Journal of Immunology, vol. 53, no. 5, pp. 489-97.

APA

Liu, H., Jensen, L., Hansen, S., Petersen, S. V., Takahashi, K., Ezekowitz, A. B., Dagnæs-Hansen, F., Jensenius, J. C., & Thiel, S. (2001). Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scandinavian Journal of Immunology, 53(5), 489-97.

CBE

Liu H, Jensen L, Hansen S, Petersen SV, Takahashi K, Ezekowitz AB, Dagnæs-Hansen F, Jensenius JC, Thiel S. 2001. Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scandinavian Journal of Immunology. 53(5):489-97.

MLA

Vancouver

Liu H, Jensen L, Hansen S, Petersen SV, Takahashi K, Ezekowitz AB et al. Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scandinavian Journal of Immunology. 2001;53(5):489-97.

Author

Liu, H ; Jensen, L ; Hansen, S ; Petersen, Steen Vang ; Takahashi, K ; Ezekowitz, A B ; Dagnæs-Hansen, Frederik ; Jensenius, J C ; Thiel, S. / Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. In: Scandinavian Journal of Immunology. 2001 ; Vol. 53, No. 5. pp. 489-97.

Bibtex

@article{f85e31f0fbd911dcb919000ea68e967b,
title = "Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses.",
abstract = "Rat monoclonal antibodies (MoAbs) against mouse mannan-binding lectin (MBL)-A and MBL-C were generated and assays for MBL-A and MBL-C were constructed. This allowed for the quantitative analysis of both proteins for the first time. Previously only MBL-A has been quantified using less standardized methods. In a mouse serum pool the concentrations were now determined at 7.5 microg MBL-A and 45 microg MBL-C per ml. On gel permeation chromatography of mouse serum, MBL-A eluted corresponding to a M(r) of 850 kDa whereas the majority of MBL-C eluted corresponding to a Mr of 950 kDa. On sucrose density gradient centrifugation the sedimentation velocities of MBL-A and MBL-C were estimated at 7.3 S and 10.8 S, respectively. The MBL-A and MBL-C levels in 10 laboratory mice strains were compared and found to vary between 4 microg/ml to 12 microg/ml, and 16 microg/ml to 118 microg/ml, respectively. After the induction of acute phase responses by intraperitoneal injection of either casein or lipopolysaccharide (LPS), MBL-A was found to increase approximately two-fold, with a maximum after 32 h, while MBL-C did not increase significantly. In comparison, serum amyloid A component (SAA) peaked at 15 h with an approximate 100-fold increase. Udgivelsesdato: 2001-May",
keywords = "Acute-Phase Proteins, Acute-Phase Reaction, Animals, Blotting, Western, Carrier Proteins, Centrifugation, Density Gradient, Chromatography, Gel, Collectins, Female, Lectins, Male, Mice, Mice, Inbred Strains, Rats, Rats, Wistar",
author = "H Liu and L Jensen and S Hansen and Petersen, {Steen Vang} and K Takahashi and Ezekowitz, {A B} and Frederik Dagn{\ae}s-Hansen and Jensenius, {J C} and S Thiel",
year = "2001",
language = "English",
volume = "53",
pages = "489--97",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "5",

}

RIS

TY - JOUR

T1 - Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses.

AU - Liu, H

AU - Jensen, L

AU - Hansen, S

AU - Petersen, Steen Vang

AU - Takahashi, K

AU - Ezekowitz, A B

AU - Dagnæs-Hansen, Frederik

AU - Jensenius, J C

AU - Thiel, S

PY - 2001

Y1 - 2001

N2 - Rat monoclonal antibodies (MoAbs) against mouse mannan-binding lectin (MBL)-A and MBL-C were generated and assays for MBL-A and MBL-C were constructed. This allowed for the quantitative analysis of both proteins for the first time. Previously only MBL-A has been quantified using less standardized methods. In a mouse serum pool the concentrations were now determined at 7.5 microg MBL-A and 45 microg MBL-C per ml. On gel permeation chromatography of mouse serum, MBL-A eluted corresponding to a M(r) of 850 kDa whereas the majority of MBL-C eluted corresponding to a Mr of 950 kDa. On sucrose density gradient centrifugation the sedimentation velocities of MBL-A and MBL-C were estimated at 7.3 S and 10.8 S, respectively. The MBL-A and MBL-C levels in 10 laboratory mice strains were compared and found to vary between 4 microg/ml to 12 microg/ml, and 16 microg/ml to 118 microg/ml, respectively. After the induction of acute phase responses by intraperitoneal injection of either casein or lipopolysaccharide (LPS), MBL-A was found to increase approximately two-fold, with a maximum after 32 h, while MBL-C did not increase significantly. In comparison, serum amyloid A component (SAA) peaked at 15 h with an approximate 100-fold increase. Udgivelsesdato: 2001-May

AB - Rat monoclonal antibodies (MoAbs) against mouse mannan-binding lectin (MBL)-A and MBL-C were generated and assays for MBL-A and MBL-C were constructed. This allowed for the quantitative analysis of both proteins for the first time. Previously only MBL-A has been quantified using less standardized methods. In a mouse serum pool the concentrations were now determined at 7.5 microg MBL-A and 45 microg MBL-C per ml. On gel permeation chromatography of mouse serum, MBL-A eluted corresponding to a M(r) of 850 kDa whereas the majority of MBL-C eluted corresponding to a Mr of 950 kDa. On sucrose density gradient centrifugation the sedimentation velocities of MBL-A and MBL-C were estimated at 7.3 S and 10.8 S, respectively. The MBL-A and MBL-C levels in 10 laboratory mice strains were compared and found to vary between 4 microg/ml to 12 microg/ml, and 16 microg/ml to 118 microg/ml, respectively. After the induction of acute phase responses by intraperitoneal injection of either casein or lipopolysaccharide (LPS), MBL-A was found to increase approximately two-fold, with a maximum after 32 h, while MBL-C did not increase significantly. In comparison, serum amyloid A component (SAA) peaked at 15 h with an approximate 100-fold increase. Udgivelsesdato: 2001-May

KW - Acute-Phase Proteins

KW - Acute-Phase Reaction

KW - Animals

KW - Blotting, Western

KW - Carrier Proteins

KW - Centrifugation, Density Gradient

KW - Chromatography, Gel

KW - Collectins

KW - Female

KW - Lectins

KW - Male

KW - Mice

KW - Mice, Inbred Strains

KW - Rats

KW - Rats, Wistar

M3 - Journal article

C2 - 11309157

VL - 53

SP - 489

EP - 497

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 5

ER -