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Jens Christian Jensenius

Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma

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Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma. / Wittenborn, Thomas; Thiel, Steffen; Jensen, Lisbeth; Nielsen, Hans J; Jensenius, Jens Christian.

In: Journal of Innate Immunity, Vol. 2, No. 2, 2010, p. 167-80.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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@article{1fb6abac7a7b41dd968c3d921641f150,
title = "Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma",
abstract = "The three human ficolins, H-ficolin, L-ficolin and M-ficolin, are pattern recognition molecules of the innate immune system. All three ficolins can activate the lectin pathway of the complement system after binding to pathogens. H- and L-ficolin are serum proteins with an average concentration of 18 and 3 microg/ml, respectively. M-ficolin has been described as a membrane-associated pattern recognition receptor of monocytes, being also present in granulocytes; recently, minuscule amounts of M-ficolin have been found in serum, too. No assay specific for M-ficolin has yet been described and biological variations are unknown. We have raised specific monoclonal anti-human M-ficolin antibodies and have developed a quantitative assay for M-ficolin. M-ficolin elutes as a large, 900-kDa protein upon gel permeation chromatography of serum. Analysis of M-ficolin levels in serum samples of 350 blood donors reveals a mean concentration of 1.07 microg/ml, ranging from 0.28 to 4.05 microg/ml. Analyses of consecutive acute phase serum samples from major surgery patients indicated a complex response. Ontogeny was investigated through cord blood samples from healthy full-term babies, which showed adult levels, with sequential samples showing no increase from birth to 1 year of age. We suggest that M-ficolin should also be considered as a humoral pattern recognition molecule.",
keywords = "Adult, Animals, Antibodies, Monoclonal, Blood Proteins, Cell Line, Cell Membrane, Female, Humans, Immunity, Innate, Lectins, Mice, Mice, Inbred BALB C, Monocytes, Receptors, Pattern Recognition",
author = "Thomas Wittenborn and Steffen Thiel and Lisbeth Jensen and Nielsen, {Hans J} and Jensenius, {Jens Christian}",
year = "2010",
doi = "10.1159/000218324",
language = "English",
volume = "2",
pages = "167--80",
journal = "Journal of Innate Immunity",
issn = "1662-811X",
publisher = "S. Karger AG",
number = "2",

}

RIS

TY - JOUR

T1 - Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma

AU - Wittenborn, Thomas

AU - Thiel, Steffen

AU - Jensen, Lisbeth

AU - Nielsen, Hans J

AU - Jensenius, Jens Christian

PY - 2010

Y1 - 2010

N2 - The three human ficolins, H-ficolin, L-ficolin and M-ficolin, are pattern recognition molecules of the innate immune system. All three ficolins can activate the lectin pathway of the complement system after binding to pathogens. H- and L-ficolin are serum proteins with an average concentration of 18 and 3 microg/ml, respectively. M-ficolin has been described as a membrane-associated pattern recognition receptor of monocytes, being also present in granulocytes; recently, minuscule amounts of M-ficolin have been found in serum, too. No assay specific for M-ficolin has yet been described and biological variations are unknown. We have raised specific monoclonal anti-human M-ficolin antibodies and have developed a quantitative assay for M-ficolin. M-ficolin elutes as a large, 900-kDa protein upon gel permeation chromatography of serum. Analysis of M-ficolin levels in serum samples of 350 blood donors reveals a mean concentration of 1.07 microg/ml, ranging from 0.28 to 4.05 microg/ml. Analyses of consecutive acute phase serum samples from major surgery patients indicated a complex response. Ontogeny was investigated through cord blood samples from healthy full-term babies, which showed adult levels, with sequential samples showing no increase from birth to 1 year of age. We suggest that M-ficolin should also be considered as a humoral pattern recognition molecule.

AB - The three human ficolins, H-ficolin, L-ficolin and M-ficolin, are pattern recognition molecules of the innate immune system. All three ficolins can activate the lectin pathway of the complement system after binding to pathogens. H- and L-ficolin are serum proteins with an average concentration of 18 and 3 microg/ml, respectively. M-ficolin has been described as a membrane-associated pattern recognition receptor of monocytes, being also present in granulocytes; recently, minuscule amounts of M-ficolin have been found in serum, too. No assay specific for M-ficolin has yet been described and biological variations are unknown. We have raised specific monoclonal anti-human M-ficolin antibodies and have developed a quantitative assay for M-ficolin. M-ficolin elutes as a large, 900-kDa protein upon gel permeation chromatography of serum. Analysis of M-ficolin levels in serum samples of 350 blood donors reveals a mean concentration of 1.07 microg/ml, ranging from 0.28 to 4.05 microg/ml. Analyses of consecutive acute phase serum samples from major surgery patients indicated a complex response. Ontogeny was investigated through cord blood samples from healthy full-term babies, which showed adult levels, with sequential samples showing no increase from birth to 1 year of age. We suggest that M-ficolin should also be considered as a humoral pattern recognition molecule.

KW - Adult

KW - Animals

KW - Antibodies, Monoclonal

KW - Blood Proteins

KW - Cell Line

KW - Cell Membrane

KW - Female

KW - Humans

KW - Immunity, Innate

KW - Lectins

KW - Mice

KW - Mice, Inbred BALB C

KW - Monocytes

KW - Receptors, Pattern Recognition

U2 - 10.1159/000218324

DO - 10.1159/000218324

M3 - Journal article

VL - 2

SP - 167

EP - 180

JO - Journal of Innate Immunity

JF - Journal of Innate Immunity

SN - 1662-811X

IS - 2

ER -