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Jens Christian Jensenius

Biological variation of anti-αGal-antibodies studied by a novel Time-Resolved ImmunoFluorometric Assay

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Biological variation of anti-αGal-antibodies studied by a novel Time-Resolved ImmunoFluorometric Assay. / Bernth-Jensen, Jens Magnus; Møller, Bjarne Kuno; Jensenius, Jens Christian; Thiel, Steffen.

In: Journal of Immunological Methods, 30.07.2011.

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@article{bbe1c67e0e7948cea84db6c107623dc1,
title = "Biological variation of anti-αGal-antibodies studied by a novel Time-Resolved ImmunoFluorometric Assay",
abstract = "As much as one percent of antibodies in human plasma are reported to be specific for the non-human disaccharide structure αGal. Various microbes express αGal. However, the implications of anti-αGal antibodies for the anti-microbial defenses are poorly established. With the perspective of studying the biological importance of the antibodies, we have established a sensitive Time-Resolved ImmunoFluorometric Assay (TRIFMA) for quantification of such antibodies. Two versions were developed, one for IgM antibodies and one for IgG antibodies. Samples were collected from plasma donations of healthy adults (n=120) of known gender (60+60), AB0-type (0: 15+15, A: 15+15, B: 15+15, and AB: 15+15) and age (19-64yrs). We subsequently examined the potential association between antibody concentration and AB0-type, gender, age, and titers of antibodies to blood type antigens. We found that IgG and IgM anti-αGal concentrations are, 1) stable over time within the individual, 2) vary more than 400-fold between individuals, 3) negatively correlated with age for IgM but not for IgG antibodies, 4) IgM antibodies are 2-fold higher in females whereas no gender difference was observed for the IgG antibodies, 5) inter-mutual correlated, 6) lowest in individuals expressing B-antigen, and 7) AB0-type A individuals may constitute an intermediate group. Our established method and findings pave the way for further studies of the involvement of anti-αGal antibodies in immunity and may be a method to examine the potential of an individual to mount an anti-carbohydrate response.",
author = "Bernth-Jensen, {Jens Magnus} and M{\o}ller, {Bjarne Kuno} and Jensenius, {Jens Christian} and Steffen Thiel",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2011",
month = jul,
day = "30",
doi = "10.1016/j.jim.2011.07.017",
language = "English",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Biological variation of anti-αGal-antibodies studied by a novel Time-Resolved ImmunoFluorometric Assay

AU - Bernth-Jensen, Jens Magnus

AU - Møller, Bjarne Kuno

AU - Jensenius, Jens Christian

AU - Thiel, Steffen

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011/7/30

Y1 - 2011/7/30

N2 - As much as one percent of antibodies in human plasma are reported to be specific for the non-human disaccharide structure αGal. Various microbes express αGal. However, the implications of anti-αGal antibodies for the anti-microbial defenses are poorly established. With the perspective of studying the biological importance of the antibodies, we have established a sensitive Time-Resolved ImmunoFluorometric Assay (TRIFMA) for quantification of such antibodies. Two versions were developed, one for IgM antibodies and one for IgG antibodies. Samples were collected from plasma donations of healthy adults (n=120) of known gender (60+60), AB0-type (0: 15+15, A: 15+15, B: 15+15, and AB: 15+15) and age (19-64yrs). We subsequently examined the potential association between antibody concentration and AB0-type, gender, age, and titers of antibodies to blood type antigens. We found that IgG and IgM anti-αGal concentrations are, 1) stable over time within the individual, 2) vary more than 400-fold between individuals, 3) negatively correlated with age for IgM but not for IgG antibodies, 4) IgM antibodies are 2-fold higher in females whereas no gender difference was observed for the IgG antibodies, 5) inter-mutual correlated, 6) lowest in individuals expressing B-antigen, and 7) AB0-type A individuals may constitute an intermediate group. Our established method and findings pave the way for further studies of the involvement of anti-αGal antibodies in immunity and may be a method to examine the potential of an individual to mount an anti-carbohydrate response.

AB - As much as one percent of antibodies in human plasma are reported to be specific for the non-human disaccharide structure αGal. Various microbes express αGal. However, the implications of anti-αGal antibodies for the anti-microbial defenses are poorly established. With the perspective of studying the biological importance of the antibodies, we have established a sensitive Time-Resolved ImmunoFluorometric Assay (TRIFMA) for quantification of such antibodies. Two versions were developed, one for IgM antibodies and one for IgG antibodies. Samples were collected from plasma donations of healthy adults (n=120) of known gender (60+60), AB0-type (0: 15+15, A: 15+15, B: 15+15, and AB: 15+15) and age (19-64yrs). We subsequently examined the potential association between antibody concentration and AB0-type, gender, age, and titers of antibodies to blood type antigens. We found that IgG and IgM anti-αGal concentrations are, 1) stable over time within the individual, 2) vary more than 400-fold between individuals, 3) negatively correlated with age for IgM but not for IgG antibodies, 4) IgM antibodies are 2-fold higher in females whereas no gender difference was observed for the IgG antibodies, 5) inter-mutual correlated, 6) lowest in individuals expressing B-antigen, and 7) AB0-type A individuals may constitute an intermediate group. Our established method and findings pave the way for further studies of the involvement of anti-αGal antibodies in immunity and may be a method to examine the potential of an individual to mount an anti-carbohydrate response.

U2 - 10.1016/j.jim.2011.07.017

DO - 10.1016/j.jim.2011.07.017

M3 - Journal article

C2 - 21835180

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -