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Jens Christian Jensenius

Assays for the mannan-binding lectin pathway

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This unit contains protocols that can be used to measure mannan-binding lectin (MBL) levels and MBL pathway activity in human plasma or serum. Using a modification of the conventional ELISA, the detection reagent used in the methods described (e.g., an antibody or streptavidin) is labeled with Eu3+ instead of an enzyme. The sensitizing reagent used in these assays employ the use of anti-MBL antibody or mannan. The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway.
Original languageEnglish
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 13
Pages (from-to)Unit 13.6
DOIs
Publication statusPublished - 2004

    Research areas

  • Complement Pathway, Mannose-Binding Lectin, Enzyme-Linked Immunosorbent Assay, Humans, Mannose-Binding Lectin, Mannose-Binding Protein-Associated Serine Proteases

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