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Jens Christian Jensenius

Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165)

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Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165). / Erb, Karin; Ditzel, H; Waever-Rasmussen, J; Borup-Christensen, P; Jensenius, Jens Christian.

In: Human antibodies and hybridomas, Vol. 2, No. 4, 1991, p. 215-21.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Erb, K, Ditzel, H, Waever-Rasmussen, J, Borup-Christensen, P & Jensenius, JC 1991, 'Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165)', Human antibodies and hybridomas, vol. 2, no. 4, pp. 215-21.

APA

Erb, K., Ditzel, H., Waever-Rasmussen, J., Borup-Christensen, P., & Jensenius, J. C. (1991). Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165). Human antibodies and hybridomas, 2(4), 215-21.

CBE

Erb K, Ditzel H, Waever-Rasmussen J, Borup-Christensen P, Jensenius JC. 1991. Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165). Human antibodies and hybridomas. 2(4):215-21.

MLA

Erb, Karin et al. "Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165)". Human antibodies and hybridomas. 1991, 2(4). 215-21.

Vancouver

Erb K, Ditzel H, Waever-Rasmussen J, Borup-Christensen P, Jensenius JC. Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165). Human antibodies and hybridomas. 1991;2(4):215-21.

Author

Erb, Karin ; Ditzel, H ; Waever-Rasmussen, J ; Borup-Christensen, P ; Jensenius, Jens Christian. / Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165). In: Human antibodies and hybridomas. 1991 ; Vol. 2, No. 4. pp. 215-21.

Bibtex

@article{dabe291375ed4eabb070eb835bf80ed0,
title = "Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165)",
abstract = "We have compared by SDS-PAGE Western blotting the molecules detected by two human monoclonal antibodies, C-OU1 and 16.88. The antibodies have previously been shown to detect a cytoplasmatic antigen with an Mr of 43 kD present in colon adenocarcinoma cell lines and in colon cancer tissues. We now demonstrate that these antibodies differ significantly in their fine specificity, resulting in a quite dissimilar tumor selectivity. The antibody 16.88, in addition to reactivity with the 43-kD molecule, also recognizes a 190-kD molecule present both in melanoma cells and in cells previously reported as 16.88 antigen positive. The 16.88 antibody does not detect a 43-kD molecule in extracts of melanoma cells. The 190-kD component was not detectable in hepatoma or mamma carcinoma cells, both of which showed presence of the 43-kD molecule. The C-OU1 antibody shows no reactivity with the 190-kD molecule in any of the cells tested or with other proteins in melanoma cells. Radiolabeled 16.88 antibody shows better localization to melanoma cancer than to colon cancer xenograft transplanted onto nude mice. These findings indicate the presence of a tumor-associated antigen not previously described and have obvious implications for potential clinical uses of the antibodies.",
keywords = "Animals, Antibodies, Monoclonal, Antibody Specificity, Antigens, Neoplasm, Colonic Neoplasms, Humans, Immunoglobulin M, Mice, Mice, Nude, Neoplasm Transplantation, Tissue Distribution, Tumor Cells, Cultured",
author = "Karin Erb and H Ditzel and J Waever-Rasmussen and P Borup-Christensen and Jensenius, {Jens Christian}",
year = "1991",
language = "English",
volume = "2",
pages = "215--21",
journal = "Human antibodies and hybridomas",
issn = "0956-960X",
publisher = "Butterworth Scientific Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Antigens recognized by two human monoclonal IgM anticolon cancer antibodies, 16.88 and C-OU1 (B9165)

AU - Erb, Karin

AU - Ditzel, H

AU - Waever-Rasmussen, J

AU - Borup-Christensen, P

AU - Jensenius, Jens Christian

PY - 1991

Y1 - 1991

N2 - We have compared by SDS-PAGE Western blotting the molecules detected by two human monoclonal antibodies, C-OU1 and 16.88. The antibodies have previously been shown to detect a cytoplasmatic antigen with an Mr of 43 kD present in colon adenocarcinoma cell lines and in colon cancer tissues. We now demonstrate that these antibodies differ significantly in their fine specificity, resulting in a quite dissimilar tumor selectivity. The antibody 16.88, in addition to reactivity with the 43-kD molecule, also recognizes a 190-kD molecule present both in melanoma cells and in cells previously reported as 16.88 antigen positive. The 16.88 antibody does not detect a 43-kD molecule in extracts of melanoma cells. The 190-kD component was not detectable in hepatoma or mamma carcinoma cells, both of which showed presence of the 43-kD molecule. The C-OU1 antibody shows no reactivity with the 190-kD molecule in any of the cells tested or with other proteins in melanoma cells. Radiolabeled 16.88 antibody shows better localization to melanoma cancer than to colon cancer xenograft transplanted onto nude mice. These findings indicate the presence of a tumor-associated antigen not previously described and have obvious implications for potential clinical uses of the antibodies.

AB - We have compared by SDS-PAGE Western blotting the molecules detected by two human monoclonal antibodies, C-OU1 and 16.88. The antibodies have previously been shown to detect a cytoplasmatic antigen with an Mr of 43 kD present in colon adenocarcinoma cell lines and in colon cancer tissues. We now demonstrate that these antibodies differ significantly in their fine specificity, resulting in a quite dissimilar tumor selectivity. The antibody 16.88, in addition to reactivity with the 43-kD molecule, also recognizes a 190-kD molecule present both in melanoma cells and in cells previously reported as 16.88 antigen positive. The 16.88 antibody does not detect a 43-kD molecule in extracts of melanoma cells. The 190-kD component was not detectable in hepatoma or mamma carcinoma cells, both of which showed presence of the 43-kD molecule. The C-OU1 antibody shows no reactivity with the 190-kD molecule in any of the cells tested or with other proteins in melanoma cells. Radiolabeled 16.88 antibody shows better localization to melanoma cancer than to colon cancer xenograft transplanted onto nude mice. These findings indicate the presence of a tumor-associated antigen not previously described and have obvious implications for potential clinical uses of the antibodies.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antibody Specificity

KW - Antigens, Neoplasm

KW - Colonic Neoplasms

KW - Humans

KW - Immunoglobulin M

KW - Mice

KW - Mice, Nude

KW - Neoplasm Transplantation

KW - Tissue Distribution

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 1751784

VL - 2

SP - 215

EP - 221

JO - Human antibodies and hybridomas

JF - Human antibodies and hybridomas

SN - 0956-960X

IS - 4

ER -