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Jens Christian Jensenius

Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA

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Standard

Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA. / Kemp, Mette; Husby, Sofie; Jensenius, Jens Christian; Rasmussen, G G; Svehag, S E.

In: Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology, Vol. 93, No. 5, 1985, p. 217-23.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Kemp, M, Husby, S, Jensenius, JC, Rasmussen, GG & Svehag, SE 1985, 'Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA', Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology, vol. 93, no. 5, pp. 217-23.

APA

Kemp, M., Husby, S., Jensenius, J. C., Rasmussen, G. G., & Svehag, S. E. (1985). Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA. Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology, 93(5), 217-23.

CBE

Kemp M, Husby S, Jensenius JC, Rasmussen GG, Svehag SE. 1985. Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA. Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology. 93(5):217-23.

MLA

Kemp, Mette et al. "Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA". Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology. 1985, 93(5). 217-23.

Vancouver

Kemp M, Husby S, Jensenius JC, Rasmussen GG, Svehag SE. Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA. Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology. 1985;93(5):217-23.

Author

Kemp, Mette ; Husby, Sofie ; Jensenius, Jens Christian ; Rasmussen, G G ; Svehag, S E. / Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA. In: Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology. 1985 ; Vol. 93, No. 5. pp. 217-23.

Bibtex

@article{e48bb309b83e4e9b88d9df51ff58f6c4,
title = "Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA",
abstract = "A one day enzyme immunoassay for the detection of rheumatoid factors of the IgG, IgM, and IgA class is described. The assay utilizes rabbit IgG as solid-phase reactant and the biotin-avidin interaction for the coupling of enzyme to indicator antibody. Three different indicator antibodies discriminated effectively between rheumatoid arthritis patients and normal subjects. F(ab')2 fragments of goat antibodies were found best suited for the test. Rheumatoid factor activity was expressed in U/ml by comparing samples to an internal standard, which was related to the WHO international reference serum for rheumatoid arthritis. Rheumatoid factor activity (U/ml) in the IgM-specific assay showed a close correlation to the latex agglutination titer. Avidity indices estimated from the slopes of the dose response curves of test sera were significantly higher for rheumatoid arthritis patients than for a group of healthy persons, indicating a higher avidity of the rheumatoid factors in the patients' sera.",
keywords = "Adult, Aged, Animals, Antibodies, Antibody Affinity, Antibody Specificity, Avidin, Biotin, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Female, Goats, Humans, Immunoglobulin A, Immunoglobulin Allotypes, Immunoglobulin G, Immunoglobulin M, Latex Fixation Tests, Male, Middle Aged, Rabbits, Rheumatoid Factor",
author = "Mette Kemp and Sofie Husby and Jensenius, {Jens Christian} and Rasmussen, {G G} and Svehag, {S E}",
year = "1985",
language = "English",
volume = "93",
pages = "217--23",
journal = "Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology",
issn = "0108-0202",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Analysis of rheumatoid factors by a biotin-avidin based isotype-specific ELISA

AU - Kemp, Mette

AU - Husby, Sofie

AU - Jensenius, Jens Christian

AU - Rasmussen, G G

AU - Svehag, S E

PY - 1985

Y1 - 1985

N2 - A one day enzyme immunoassay for the detection of rheumatoid factors of the IgG, IgM, and IgA class is described. The assay utilizes rabbit IgG as solid-phase reactant and the biotin-avidin interaction for the coupling of enzyme to indicator antibody. Three different indicator antibodies discriminated effectively between rheumatoid arthritis patients and normal subjects. F(ab')2 fragments of goat antibodies were found best suited for the test. Rheumatoid factor activity was expressed in U/ml by comparing samples to an internal standard, which was related to the WHO international reference serum for rheumatoid arthritis. Rheumatoid factor activity (U/ml) in the IgM-specific assay showed a close correlation to the latex agglutination titer. Avidity indices estimated from the slopes of the dose response curves of test sera were significantly higher for rheumatoid arthritis patients than for a group of healthy persons, indicating a higher avidity of the rheumatoid factors in the patients' sera.

AB - A one day enzyme immunoassay for the detection of rheumatoid factors of the IgG, IgM, and IgA class is described. The assay utilizes rabbit IgG as solid-phase reactant and the biotin-avidin interaction for the coupling of enzyme to indicator antibody. Three different indicator antibodies discriminated effectively between rheumatoid arthritis patients and normal subjects. F(ab')2 fragments of goat antibodies were found best suited for the test. Rheumatoid factor activity was expressed in U/ml by comparing samples to an internal standard, which was related to the WHO international reference serum for rheumatoid arthritis. Rheumatoid factor activity (U/ml) in the IgM-specific assay showed a close correlation to the latex agglutination titer. Avidity indices estimated from the slopes of the dose response curves of test sera were significantly higher for rheumatoid arthritis patients than for a group of healthy persons, indicating a higher avidity of the rheumatoid factors in the patients' sera.

KW - Adult

KW - Aged

KW - Animals

KW - Antibodies

KW - Antibody Affinity

KW - Antibody Specificity

KW - Avidin

KW - Biotin

KW - Cross Reactions

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Goats

KW - Humans

KW - Immunoglobulin A

KW - Immunoglobulin Allotypes

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Latex Fixation Tests

KW - Male

KW - Middle Aged

KW - Rabbits

KW - Rheumatoid Factor

M3 - Journal article

C2 - 4083017

VL - 93

SP - 217

EP - 223

JO - Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology

JF - Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology

SN - 0108-0202

IS - 5

ER -