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Jens Christian Jensenius

A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies

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Standard

A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies. / Ditzel, H; Erb, Karin; Nielsen, B; Borup-Christensen, P; Jensenius, Jens Christian.

In: Journal of Immunological Methods, Vol. 133, No. 2, 1990, p. 245-51.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Ditzel, H, Erb, K, Nielsen, B, Borup-Christensen, P & Jensenius, JC 1990, 'A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies', Journal of Immunological Methods, vol. 133, no. 2, pp. 245-51.

APA

Ditzel, H., Erb, K., Nielsen, B., Borup-Christensen, P., & Jensenius, J. C. (1990). A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies. Journal of Immunological Methods, 133(2), 245-51.

CBE

Ditzel H, Erb K, Nielsen B, Borup-Christensen P, Jensenius JC. 1990. A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies. Journal of Immunological Methods. 133(2):245-51.

MLA

Vancouver

Ditzel H, Erb K, Nielsen B, Borup-Christensen P, Jensenius JC. A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies. Journal of Immunological Methods. 1990;133(2):245-51.

Author

Ditzel, H ; Erb, Karin ; Nielsen, B ; Borup-Christensen, P ; Jensenius, Jens Christian. / A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies. In: Journal of Immunological Methods. 1990 ; Vol. 133, No. 2. pp. 245-51.

Bibtex

@article{57fcff2d6ec8458c98ea47f6542b4880,
title = "A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies",
abstract = "Using an indirect immunoperoxidase technique antigen independent binding of both human monoclonal and polyclonal IgM was found to a wide range of frozen sections of normal and malignant human glandular epithelia. Identical binding was found using dimeric human IgA (dIgA), whereas no binding was found with monomeric human IgA or human IgG. Secretory component (SC) was found to be the component in these tissues mediating antigen-independent binding of human IgM and dIgA antibodies. A method for the blocking of this antigen-independent binding of human IgM and dIgA was evaluated. Frozen sections of tissues containing SC were blocked with antibody to endogenous immunoglobulin and preincubated with rabbit anti-secretory component antibody (anti-SC) before applying the human monoclonal antibody. This treatment blocked the binding of control polyclonal and monoclonal human IgM to sections of SC-containing tissues such as respiratory and colonic epithelia. The influence of anti-SC on the binding of dIgA could not be established due to interactions between the anti-SC antibody and the IgA preparation. Using this method human hybridoma supernatants containing IgM could be readily screened for reactivity with frozen tissue sections from patients with colo-rectal cancer. This approach is recommended for the screening of human IgM monoclonal antibodies on frozen human tissue sections.",
keywords = "Animals, Antibodies, Monoclonal, Epithelium, Freezing, Humans, Hybridomas, Immunoenzyme Techniques, Immunoglobulin A, Immunoglobulin M, Immunohistochemistry, Rabbits, Secretory Component, Tumor Cells, Cultured",
author = "H Ditzel and Karin Erb and B Nielsen and P Borup-Christensen and Jensenius, {Jens Christian}",
year = "1990",
language = "English",
volume = "133",
pages = "245--51",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - A method for blocking antigen-independent binding of human IgM to frozen tissue sections when screening human hybridoma antibodies

AU - Ditzel, H

AU - Erb, Karin

AU - Nielsen, B

AU - Borup-Christensen, P

AU - Jensenius, Jens Christian

PY - 1990

Y1 - 1990

N2 - Using an indirect immunoperoxidase technique antigen independent binding of both human monoclonal and polyclonal IgM was found to a wide range of frozen sections of normal and malignant human glandular epithelia. Identical binding was found using dimeric human IgA (dIgA), whereas no binding was found with monomeric human IgA or human IgG. Secretory component (SC) was found to be the component in these tissues mediating antigen-independent binding of human IgM and dIgA antibodies. A method for the blocking of this antigen-independent binding of human IgM and dIgA was evaluated. Frozen sections of tissues containing SC were blocked with antibody to endogenous immunoglobulin and preincubated with rabbit anti-secretory component antibody (anti-SC) before applying the human monoclonal antibody. This treatment blocked the binding of control polyclonal and monoclonal human IgM to sections of SC-containing tissues such as respiratory and colonic epithelia. The influence of anti-SC on the binding of dIgA could not be established due to interactions between the anti-SC antibody and the IgA preparation. Using this method human hybridoma supernatants containing IgM could be readily screened for reactivity with frozen tissue sections from patients with colo-rectal cancer. This approach is recommended for the screening of human IgM monoclonal antibodies on frozen human tissue sections.

AB - Using an indirect immunoperoxidase technique antigen independent binding of both human monoclonal and polyclonal IgM was found to a wide range of frozen sections of normal and malignant human glandular epithelia. Identical binding was found using dimeric human IgA (dIgA), whereas no binding was found with monomeric human IgA or human IgG. Secretory component (SC) was found to be the component in these tissues mediating antigen-independent binding of human IgM and dIgA antibodies. A method for the blocking of this antigen-independent binding of human IgM and dIgA was evaluated. Frozen sections of tissues containing SC were blocked with antibody to endogenous immunoglobulin and preincubated with rabbit anti-secretory component antibody (anti-SC) before applying the human monoclonal antibody. This treatment blocked the binding of control polyclonal and monoclonal human IgM to sections of SC-containing tissues such as respiratory and colonic epithelia. The influence of anti-SC on the binding of dIgA could not be established due to interactions between the anti-SC antibody and the IgA preparation. Using this method human hybridoma supernatants containing IgM could be readily screened for reactivity with frozen tissue sections from patients with colo-rectal cancer. This approach is recommended for the screening of human IgM monoclonal antibodies on frozen human tissue sections.

KW - Animals

KW - Antibodies, Monoclonal

KW - Epithelium

KW - Freezing

KW - Humans

KW - Hybridomas

KW - Immunoenzyme Techniques

KW - Immunoglobulin A

KW - Immunoglobulin M

KW - Immunohistochemistry

KW - Rabbits

KW - Secretory Component

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 2230141

VL - 133

SP - 245

EP - 251

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -