Jakob Tobias Nyvad

Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


  • Sophie N Saxton, Division of Cardiovascular Sciences, University of Manchester, Manchester, United Kingdom.
  • ,
  • Sarah B Withers, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom.
  • ,
  • Jakob Nyvad
  • Aleksandra Mazur
  • ,
  • Vladimir Matchkov
  • Anthony M Heagerty, Division of Cardiovascular Sciences, University of Manchester, Manchester, United Kingdom.
  • ,
  • Christian Aalkjær

BACKGROUND: Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte β3-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin.

OBJECTIVE: We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of β3-adrenoceptors and adiponectin.

METHOD: In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach).

RESULTS: We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using β3-adrenoceptor inhibition, and activation of β3-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The β3-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon β3-adrenoceptor activation.

CONCLUSIONS: These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte β3-adrenoceptors, which may trigger release of the vasodilator adiponectin.

Original languageEnglish
JournalJournal of Vascular Research
Pages (from-to)320-332
Number of pages13
Publication statusPublished - 2019

    Research areas

  • Adipocytes, Adrenoceptors, Vascular tone

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