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Inger Holme

Overexpression of nepenthesin HvNEP-1 in barley endosperm reduces fusarium head blight and mycotoxin accumulation

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Overexpression of nepenthesin HvNEP-1 in barley endosperm reduces fusarium head blight and mycotoxin accumulation. / Bekalu, Zelalem Eshetu; Madsen, Claus Krogh; Dionisio, Giuseppe; Holme, Inger Bæksted; Jørgensen, Lise Nistrup; Fomsgaard, Inge S.; Brinch-Pedersen, Henrik.

In: Agronomy, Vol. 10, No. 2, 203, 02.2020.

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@article{82468fdf10f14581a08cb7fa69eb416a,
title = "Overexpression of nepenthesin HvNEP-1 in barley endosperm reduces fusarium head blight and mycotoxin accumulation",
abstract = "Fusarium head blight (FHB) causes substantial losses of yield and quality in grains, both in the field and in post-harvest storage. To date, adequate natural genetic resistance is not available for the control of FHB. This study reports the cloning and overexpression of a barley (Hordeum vulgare L.) antifungal gene, nepenthesin 1 (HvNEP-1), in the endosperm of barley grains. Transgenic barley lines overexpressing HvNEP-1 substantially reduced FHB severity and disease progression after inoculation with Fusarium graminearum or Fusarium culmorum. The transgenic barley also showed reduced accumulation of the mycotoxin deoxynivalenol (DON) in grain, far below the minimum value allowable for food. Semi-field evaluation of four HvNEP-1 transgenic lines revealed substantial reduction of FHB severity and progression as compared with the control H. vulgare cultivar Golden promise (GP) plants. Our study demonstrated the utility of HvNEP-1 for the control of FHB in barley, and possibly other grains such as wheat and maize.",
keywords = "Endosperm, FHB, Hordeum vulgare, HvNEP-1, Mycotoxins, Overexpression, Progression, Severity",
author = "Bekalu, {Zelalem Eshetu} and Madsen, {Claus Krogh} and Giuseppe Dionisio and Holme, {Inger B{\ae}ksted} and J{\o}rgensen, {Lise Nistrup} and Fomsgaard, {Inge S.} and Henrik Brinch-Pedersen",
year = "2020",
month = feb,
doi = "10.3390/agronomy10020203",
language = "English",
volume = "10",
journal = "Agronomy-Basel",
issn = "2073-4395",
publisher = "MDPI",
number = "2",

}

RIS

TY - JOUR

T1 - Overexpression of nepenthesin HvNEP-1 in barley endosperm reduces fusarium head blight and mycotoxin accumulation

AU - Bekalu, Zelalem Eshetu

AU - Madsen, Claus Krogh

AU - Dionisio, Giuseppe

AU - Holme, Inger Bæksted

AU - Jørgensen, Lise Nistrup

AU - Fomsgaard, Inge S.

AU - Brinch-Pedersen, Henrik

PY - 2020/2

Y1 - 2020/2

N2 - Fusarium head blight (FHB) causes substantial losses of yield and quality in grains, both in the field and in post-harvest storage. To date, adequate natural genetic resistance is not available for the control of FHB. This study reports the cloning and overexpression of a barley (Hordeum vulgare L.) antifungal gene, nepenthesin 1 (HvNEP-1), in the endosperm of barley grains. Transgenic barley lines overexpressing HvNEP-1 substantially reduced FHB severity and disease progression after inoculation with Fusarium graminearum or Fusarium culmorum. The transgenic barley also showed reduced accumulation of the mycotoxin deoxynivalenol (DON) in grain, far below the minimum value allowable for food. Semi-field evaluation of four HvNEP-1 transgenic lines revealed substantial reduction of FHB severity and progression as compared with the control H. vulgare cultivar Golden promise (GP) plants. Our study demonstrated the utility of HvNEP-1 for the control of FHB in barley, and possibly other grains such as wheat and maize.

AB - Fusarium head blight (FHB) causes substantial losses of yield and quality in grains, both in the field and in post-harvest storage. To date, adequate natural genetic resistance is not available for the control of FHB. This study reports the cloning and overexpression of a barley (Hordeum vulgare L.) antifungal gene, nepenthesin 1 (HvNEP-1), in the endosperm of barley grains. Transgenic barley lines overexpressing HvNEP-1 substantially reduced FHB severity and disease progression after inoculation with Fusarium graminearum or Fusarium culmorum. The transgenic barley also showed reduced accumulation of the mycotoxin deoxynivalenol (DON) in grain, far below the minimum value allowable for food. Semi-field evaluation of four HvNEP-1 transgenic lines revealed substantial reduction of FHB severity and progression as compared with the control H. vulgare cultivar Golden promise (GP) plants. Our study demonstrated the utility of HvNEP-1 for the control of FHB in barley, and possibly other grains such as wheat and maize.

KW - Endosperm

KW - FHB

KW - Hordeum vulgare

KW - HvNEP-1

KW - Mycotoxins

KW - Overexpression

KW - Progression

KW - Severity

UR - http://www.scopus.com/inward/record.url?scp=85078971324&partnerID=8YFLogxK

U2 - 10.3390/agronomy10020203

DO - 10.3390/agronomy10020203

M3 - Journal article

AN - SCOPUS:85078971324

VL - 10

JO - Agronomy-Basel

JF - Agronomy-Basel

SN - 2073-4395

IS - 2

M1 - 203

ER -