About Aarhus University
Henrik Balslev
Antibacterial property testing of two species of tropical plant Lasianthus (Rubiaceae)
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In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 48, No. 1, 01.2017, p. 117-123.
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
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TY - JOUR
T1 - Antibacterial property testing of two species of tropical plant Lasianthus (Rubiaceae)
AU - Napiroon, Tiwtawat
AU - Balslev, Henrik
AU - Duangjai, Sutee
AU - Sookchaloem, Duangchai
AU - Chayamarit, Kongkanda
AU - Santemaleeworangun, Wichai
AU - Vayrodaya, Srunya
PY - 2017/1
Y1 - 2017/1
N2 - Two tropical plant species, Lasianthus pilosus and Lasianthus stipularis are used in traditional medicine but there have been no published studies of the extracts of these plants against bacteria. In this study, we aimed to determine the antimicrobial activities of the above two plants against two gram-positive and seven gram-negative bacterial strains to determine the potential of these two plant species for possible antimicrobial drug development. The antibacterial activities of the lipophilic extracts of these plants were evaluated by disk diffusion and broth microdilution methods. The zone diameters and minimum inhibitory concentrations (MIC) for these plant extracts exhibited their highest antibacterial activity against Pseudomonas aeruginosa, followed by Staphylococcus aureus and Acinetobacter baumannii, respectively. The MIC of these extracts against P. aeruginosa (ATCC 37166 and ATCC 27853) varied from 50 to 200 µg/ml. Thin layer chromatography and detection using different specific reagents revealed the presence of terpenoids, phenolic compounds and iridoid. Cell lysis due to the effect of the lipophilic extracts of these tested plants was demonstrated using scanning electron microscopy. In conclusion, the bioactive compounds of these plants should be studied further to develop potential antimicrobial agents.
AB - Two tropical plant species, Lasianthus pilosus and Lasianthus stipularis are used in traditional medicine but there have been no published studies of the extracts of these plants against bacteria. In this study, we aimed to determine the antimicrobial activities of the above two plants against two gram-positive and seven gram-negative bacterial strains to determine the potential of these two plant species for possible antimicrobial drug development. The antibacterial activities of the lipophilic extracts of these plants were evaluated by disk diffusion and broth microdilution methods. The zone diameters and minimum inhibitory concentrations (MIC) for these plant extracts exhibited their highest antibacterial activity against Pseudomonas aeruginosa, followed by Staphylococcus aureus and Acinetobacter baumannii, respectively. The MIC of these extracts against P. aeruginosa (ATCC 37166 and ATCC 27853) varied from 50 to 200 µg/ml. Thin layer chromatography and detection using different specific reagents revealed the presence of terpenoids, phenolic compounds and iridoid. Cell lysis due to the effect of the lipophilic extracts of these tested plants was demonstrated using scanning electron microscopy. In conclusion, the bioactive compounds of these plants should be studied further to develop potential antimicrobial agents.
KW - Lasianthus, medicinal plants, pathogenic bacteria
M3 - Journal article
VL - 48
SP - 117
EP - 123
JO - Southeast Asian Journal of Tropical Medicine and Public Health
JF - Southeast Asian Journal of Tropical Medicine and Public Health
SN - 0125-1562
IS - 1
ER -