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Hans-Jörg Schulz

Visualizing uncertainty in biological expression data

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DOI

  • Clemens Holzhüter
  • ,
  • Alexander Lex
  • ,
  • Dieter Schmalstieg
  • ,
  • Hans-Jörg Schulz
  • Heidrun Schumann
  • ,
  • Marc Streit
Expression analysis of textasciitildeomics data using microarrays has become a standard procedure in the life sciences. However, microarrays are subject to technical limitations and errors, which render the data gathered likely to be uncertain. While a number of approaches exist to target this uncertainty statistically, it is hardly ever even shown when the data is visualized using for example clustered heatmaps. Yet, this is highly useful when trying not to omit data that is "good enough" for an analysis, which otherwise would be discarded as too unreliable by established conservative thresholds. Our approach addresses this shortcoming by first identifying the margin above the error threshold of uncertain, yet possibly still useful data. It then displays this uncertain data in the context of the valid data by enhancing a clustered heatmap. We employ different visual representations for the different kinds of uncertainty involved. Finally, it lets the user interactively adjust the thresholds, giving visual feedback in the heatmap representation, so that an informed choice on which thresholds to use can be made instead of applying the usual rule-of-thumb cut-offs. We exemplify the usefulness of our concept by giving details for a concrete use case from our partners at the Medical University of Graz, thereby demonstrating our implementation of the general approach.
Original languageEnglish
Title of host publicationProceedings of the Conference on Visualization and Data Analysis VDA 2012
EditorsPak Chung Wong, David L. Kao, Ming C. Hao, Chaomei Chen, Robert Kosara, Mark A. Livingston, Jinah Park, Ian Roberts
Number of pages11
Place of publicationBurlingame, CA, USA
PublisherSPIE
Publication year2012
Chapter82940O
DOIs
Publication statusPublished - 2012

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