Hans Jürgen Hoffmann

PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes

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PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. / Foldbjerg, Rasmus; Olesen, Ping Liu; Hougaard, Mads; Dang, Duy Anh; Hoffmann, Hans Jürgen; Autrup, Herman.

In: Toxicology Letters, Vol. 190, No. 2, 28.10.2009, p. 156-162.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Foldbjerg, Rasmus ; Olesen, Ping Liu ; Hougaard, Mads ; Dang, Duy Anh ; Hoffmann, Hans Jürgen ; Autrup, Herman. / PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. In: Toxicology Letters. 2009 ; Vol. 190, No. 2. pp. 156-162.

Bibtex

@article{1fed0b50865e11deb4c2000ea68e967b,
title = "PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes",
abstract = "The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs) in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69 nm ± 3 nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24 h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.",
keywords = "silver, nanoparticles, apoptosis, reactive oxygen species, monocytes",
author = "Rasmus Foldbjerg and Olesen, {Ping Liu} and Mads Hougaard and Dang, {Duy Anh} and Hoffmann, {Hans J{\"u}rgen} and Herman Autrup",
year = "2009",
month = oct,
day = "28",
doi = "10.1016/j.toxlet.2009.07.009",
language = "English",
volume = "190",
pages = "156--162",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier Ireland Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes

AU - Foldbjerg, Rasmus

AU - Olesen, Ping Liu

AU - Hougaard, Mads

AU - Dang, Duy Anh

AU - Hoffmann, Hans Jürgen

AU - Autrup, Herman

PY - 2009/10/28

Y1 - 2009/10/28

N2 - The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs) in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69 nm ± 3 nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24 h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.

AB - The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs) in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69 nm ± 3 nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24 h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.

KW - silver

KW - nanoparticles

KW - apoptosis

KW - reactive oxygen species

KW - monocytes

U2 - 10.1016/j.toxlet.2009.07.009

DO - 10.1016/j.toxlet.2009.07.009

M3 - Journal article

C2 - 19607894

VL - 190

SP - 156

EP - 162

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 2

ER -