Hans Jürgen Hoffmann

Increasing time interval and decreasing allergen dose interval improves ex vivo desensitization of human blood basophils

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Increasing time interval and decreasing allergen dose interval improves ex vivo desensitization of human blood basophils. / Witting Christensen, Sara K; Krohn, Inge Kortekaas; Thuraiaiyah, Jani; Skjold, Tina; Schmid, Johannes Martin; Hoffmann, Hans Jürgen H.

In: Cytometry. Part B: Clinical Cytometry, Vol. 92, No. 5 , 09.2017, p. 340-347.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Witting Christensen, SK, Krohn, IK, Thuraiaiyah, J, Skjold, T, Schmid, JM & Hoffmann, HJH 2017, 'Increasing time interval and decreasing allergen dose interval improves ex vivo desensitization of human blood basophils', Cytometry. Part B: Clinical Cytometry, vol. 92, no. 5 , pp. 340-347. https://doi.org/10.1002/cyto.b.21377

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Witting Christensen, Sara K ; Krohn, Inge Kortekaas ; Thuraiaiyah, Jani ; Skjold, Tina ; Schmid, Johannes Martin ; Hoffmann, Hans Jürgen H. / Increasing time interval and decreasing allergen dose interval improves ex vivo desensitization of human blood basophils. In: Cytometry. Part B: Clinical Cytometry. 2017 ; Vol. 92, No. 5 . pp. 340-347.

Bibtex

@article{13647c62413e4681bc6ccc2339a79f24,
title = "Increasing time interval and decreasing allergen dose interval improves ex vivo desensitization of human blood basophils",
abstract = "BACKGROUND: Desensitization is a method for inducing temporary tolerance to allergen. The mechanism underlying desensitization is yet to be established.METHODS: Basophil granulocytes in whole blood from grass pollen allergic subjects were desensitized ex vivo by sequential addition of increasing allergen concentrations. At each step basophil activation (CD193(+) CD63(+) ) was monitored with and without (background activation) allergen challenge at optimal concentration. The sequential desensitization protocol was compared to a single-dose desensitization protocols with threshold and subthreshold allergen concentrations. Incubation intervals and allergen concentrations were varied in order to optimise the protocol.RESULTS: Sequential desensitization effectively reduced basophil response. The single-dose subthreshold protocol and single-dose threshold protocols did not reduce basophil activation with optimal allergen challenge from a mean 57.1 (95% CI 32.7-81.5) to 50.4% (95% CI 16.3-84.4; n=5; p=0.43) and 45.0% (95% CI 23.1-66.9; p=0.14) respectively, while the sequential desensitization protocol reduced activation to a mean 37.2% (95% CI 16.3-58.1; p=0.018). Reducing incubation time from 10 to 5 minutes increased mean background activation from 22.4 (95% CI 11.7-33.1) to 30.0% (95% CI 19.7-40.3; n=5; p=0.026). Increasing time intervals from 10 to 20 minutes reduced background activation from 30.9 (95% CI 22.8-39.0) to 21.9% (95% CI 16.0-27.7; n=5; p=0.020). Increasing allergen concentrations intervals from 2-fold to 5- and 10-fold did not have significant effect on basophil activation.CONCLUSIONS: Sequential desensitization ex vivo effectively attenuates the basophil response to allergen. Increasing the time spent at each step improves desensitization. This protocol could be valuable for investigation of the mechanism of desensitization. This article is protected by copyright. All rights reserved.",
author = "{Witting Christensen}, {Sara K} and Krohn, {Inge Kortekaas} and Jani Thuraiaiyah and Tina Skjold and Schmid, {Johannes Martin} and Hoffmann, {Hans J{\"u}rgen H}",
note = "{\textcopyright} 2016 International Clinical Cytometry Society.",
year = "2017",
month = sep,
doi = "10.1002/cyto.b.21377",
language = "English",
volume = "92",
pages = "340--347",
journal = "Cytometry. Part B: Clinical Cytometry",
issn = "1552-4949",
publisher = "JohnWiley & Sons, Inc.",
number = "5 ",

}

RIS

TY - JOUR

T1 - Increasing time interval and decreasing allergen dose interval improves ex vivo desensitization of human blood basophils

AU - Witting Christensen, Sara K

AU - Krohn, Inge Kortekaas

AU - Thuraiaiyah, Jani

AU - Skjold, Tina

AU - Schmid, Johannes Martin

AU - Hoffmann, Hans Jürgen H

N1 - © 2016 International Clinical Cytometry Society.

PY - 2017/9

Y1 - 2017/9

N2 - BACKGROUND: Desensitization is a method for inducing temporary tolerance to allergen. The mechanism underlying desensitization is yet to be established.METHODS: Basophil granulocytes in whole blood from grass pollen allergic subjects were desensitized ex vivo by sequential addition of increasing allergen concentrations. At each step basophil activation (CD193(+) CD63(+) ) was monitored with and without (background activation) allergen challenge at optimal concentration. The sequential desensitization protocol was compared to a single-dose desensitization protocols with threshold and subthreshold allergen concentrations. Incubation intervals and allergen concentrations were varied in order to optimise the protocol.RESULTS: Sequential desensitization effectively reduced basophil response. The single-dose subthreshold protocol and single-dose threshold protocols did not reduce basophil activation with optimal allergen challenge from a mean 57.1 (95% CI 32.7-81.5) to 50.4% (95% CI 16.3-84.4; n=5; p=0.43) and 45.0% (95% CI 23.1-66.9; p=0.14) respectively, while the sequential desensitization protocol reduced activation to a mean 37.2% (95% CI 16.3-58.1; p=0.018). Reducing incubation time from 10 to 5 minutes increased mean background activation from 22.4 (95% CI 11.7-33.1) to 30.0% (95% CI 19.7-40.3; n=5; p=0.026). Increasing time intervals from 10 to 20 minutes reduced background activation from 30.9 (95% CI 22.8-39.0) to 21.9% (95% CI 16.0-27.7; n=5; p=0.020). Increasing allergen concentrations intervals from 2-fold to 5- and 10-fold did not have significant effect on basophil activation.CONCLUSIONS: Sequential desensitization ex vivo effectively attenuates the basophil response to allergen. Increasing the time spent at each step improves desensitization. This protocol could be valuable for investigation of the mechanism of desensitization. This article is protected by copyright. All rights reserved.

AB - BACKGROUND: Desensitization is a method for inducing temporary tolerance to allergen. The mechanism underlying desensitization is yet to be established.METHODS: Basophil granulocytes in whole blood from grass pollen allergic subjects were desensitized ex vivo by sequential addition of increasing allergen concentrations. At each step basophil activation (CD193(+) CD63(+) ) was monitored with and without (background activation) allergen challenge at optimal concentration. The sequential desensitization protocol was compared to a single-dose desensitization protocols with threshold and subthreshold allergen concentrations. Incubation intervals and allergen concentrations were varied in order to optimise the protocol.RESULTS: Sequential desensitization effectively reduced basophil response. The single-dose subthreshold protocol and single-dose threshold protocols did not reduce basophil activation with optimal allergen challenge from a mean 57.1 (95% CI 32.7-81.5) to 50.4% (95% CI 16.3-84.4; n=5; p=0.43) and 45.0% (95% CI 23.1-66.9; p=0.14) respectively, while the sequential desensitization protocol reduced activation to a mean 37.2% (95% CI 16.3-58.1; p=0.018). Reducing incubation time from 10 to 5 minutes increased mean background activation from 22.4 (95% CI 11.7-33.1) to 30.0% (95% CI 19.7-40.3; n=5; p=0.026). Increasing time intervals from 10 to 20 minutes reduced background activation from 30.9 (95% CI 22.8-39.0) to 21.9% (95% CI 16.0-27.7; n=5; p=0.020). Increasing allergen concentrations intervals from 2-fold to 5- and 10-fold did not have significant effect on basophil activation.CONCLUSIONS: Sequential desensitization ex vivo effectively attenuates the basophil response to allergen. Increasing the time spent at each step improves desensitization. This protocol could be valuable for investigation of the mechanism of desensitization. This article is protected by copyright. All rights reserved.

U2 - 10.1002/cyto.b.21377

DO - 10.1002/cyto.b.21377

M3 - Journal article

C2 - 27071698

VL - 92

SP - 340

EP - 347

JO - Cytometry. Part B: Clinical Cytometry

JF - Cytometry. Part B: Clinical Cytometry

SN - 1552-4949

IS - 5

ER -