Hans Jürgen Hoffmann

Endosomal signalling via exosome surface TGFβ-1

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Endosomal signalling via exosome surface TGFβ-1. / Shelke, Ganesh Vilas; Yin, Yanan; Jang, Su Chul; Lasser, Cecilia; Wennmalm, Stefan; Hoffmann, Hans Jurgen; Li, Li; Gho, Yong Song; Nilsson, Jonas Andreas; Lotvall, Jan.

In: Journal of Extracellular Vesicles, Vol. 8, No. 1, 1650458, 2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Shelke, GV, Yin, Y, Jang, SC, Lasser, C, Wennmalm, S, Hoffmann, HJ, Li, L, Gho, YS, Nilsson, JA & Lotvall, J 2019, 'Endosomal signalling via exosome surface TGFβ-1', Journal of Extracellular Vesicles, vol. 8, no. 1, 1650458. https://doi.org/10.1080/20013078.2019.1650458

APA

Shelke, G. V., Yin, Y., Jang, S. C., Lasser, C., Wennmalm, S., Hoffmann, H. J., Li, L., Gho, Y. S., Nilsson, J. A., & Lotvall, J. (2019). Endosomal signalling via exosome surface TGFβ-1. Journal of Extracellular Vesicles, 8(1), [1650458]. https://doi.org/10.1080/20013078.2019.1650458

CBE

Shelke GV, Yin Y, Jang SC, Lasser C, Wennmalm S, Hoffmann HJ, Li L, Gho YS, Nilsson JA, Lotvall J. 2019. Endosomal signalling via exosome surface TGFβ-1. Journal of Extracellular Vesicles. 8(1):Article 1650458. https://doi.org/10.1080/20013078.2019.1650458

MLA

Shelke, Ganesh Vilas et al. "Endosomal signalling via exosome surface TGFβ-1". Journal of Extracellular Vesicles. 2019. 8(1). https://doi.org/10.1080/20013078.2019.1650458

Vancouver

Shelke GV, Yin Y, Jang SC, Lasser C, Wennmalm S, Hoffmann HJ et al. Endosomal signalling via exosome surface TGFβ-1. Journal of Extracellular Vesicles. 2019;8(1). 1650458. https://doi.org/10.1080/20013078.2019.1650458

Author

Shelke, Ganesh Vilas ; Yin, Yanan ; Jang, Su Chul ; Lasser, Cecilia ; Wennmalm, Stefan ; Hoffmann, Hans Jurgen ; Li, Li ; Gho, Yong Song ; Nilsson, Jonas Andreas ; Lotvall, Jan. / Endosomal signalling via exosome surface TGFβ-1. In: Journal of Extracellular Vesicles. 2019 ; Vol. 8, No. 1.

Bibtex

@article{a72531f8e08d42b193cd2936c63d1a02,
title = "Endosomal signalling via exosome surface TGFβ-1",
abstract = "Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The latent form of TGFβ-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGFβ-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGFβ-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGFβ-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.",
keywords = "Mast cells, extracellular vesicles, exosomes, mesenchymal stem cells, tumour growth factor beta-1, cellular localization, endosomal signalling, proteoglycan, MESENCHYMAL STEM-CELLS, HEPARAN-SULFATE PROTEOGLYCANS, GROWTH-FACTOR-BETA, TGF-BETA, MEDIATED TRANSFER, CANCER EXOSOMES, INTERNALIZATION, FIBROBLAST, PHENOTYPE, MIGRATION",
author = "Shelke, {Ganesh Vilas} and Yanan Yin and Jang, {Su Chul} and Cecilia Lasser and Stefan Wennmalm and Hoffmann, {Hans Jurgen} and Li Li and Gho, {Yong Song} and Nilsson, {Jonas Andreas} and Jan Lotvall",
year = "2019",
doi = "10.1080/20013078.2019.1650458",
language = "English",
volume = "8",
journal = "Journal of Extracellular Vesicles",
issn = "2001-3078",
publisher = "Co-Action Publishing",
number = "1",

}

RIS

TY - JOUR

T1 - Endosomal signalling via exosome surface TGFβ-1

AU - Shelke, Ganesh Vilas

AU - Yin, Yanan

AU - Jang, Su Chul

AU - Lasser, Cecilia

AU - Wennmalm, Stefan

AU - Hoffmann, Hans Jurgen

AU - Li, Li

AU - Gho, Yong Song

AU - Nilsson, Jonas Andreas

AU - Lotvall, Jan

PY - 2019

Y1 - 2019

N2 - Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The latent form of TGFβ-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGFβ-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGFβ-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGFβ-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.

AB - Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The latent form of TGFβ-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGFβ-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGFβ-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGFβ-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.

KW - Mast cells

KW - extracellular vesicles

KW - exosomes

KW - mesenchymal stem cells

KW - tumour growth factor beta-1

KW - cellular localization

KW - endosomal signalling

KW - proteoglycan

KW - MESENCHYMAL STEM-CELLS

KW - HEPARAN-SULFATE PROTEOGLYCANS

KW - GROWTH-FACTOR-BETA

KW - TGF-BETA

KW - MEDIATED TRANSFER

KW - CANCER EXOSOMES

KW - INTERNALIZATION

KW - FIBROBLAST

KW - PHENOTYPE

KW - MIGRATION

U2 - 10.1080/20013078.2019.1650458

DO - 10.1080/20013078.2019.1650458

M3 - Journal article

C2 - 31595182

VL - 8

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

SN - 2001-3078

IS - 1

M1 - 1650458

ER -