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Giuseppe Dionisio

Member of Administrative Staff

Giuseppe Dionisio
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I am leading the facilities of Proteomics, Mass Spectroscopy, Protein chemistry/Biochemistry of the section of Crop Genetics and Biotechnology at Flakkebjerg Campus part of Department of Molecular biology and Genetics of Aarhus University. The topics of my research include plant and microbial molecular biology, proteomics, physiology and biochemistry (e.g. enzymology of plant and microbial proteins). In relationship to plant biotechnology my past achievements have been based on grain hydrolases characterization as biochemically purified from the plant sources or recombinant produced in Escherichia coli or yeast. Plant transformation with such hydrolases (Transgenic or Cis-genic approach) have validated their role in a specific biochemical pathway (i.e. purple acid phosphatases with phytase activity in phytate degradation). Other plant enzymes of interest are cereal grains hydrolases in particular different classes of proteases (aspartic, serine, cysteine types, etc.) and proteases inhibitors (i.e. chymotrypsin inhibitors, serpins, Bowmann-Birk, etc.). Their biochemical characterization, post translational modifications discovery by Mass Spectroscopy including absolute differential proteomics is currently performed under my supervision using enzymes extracted from the grains or recombinant enzymes overexpressed either in planta or in heterologous hosts (E. coli, Pichia pastoris, etc.). One of the topics in which I am currently working on is proteolytic feed enzymes from plant/fungi and their interaction with endogenous cereal proteinaceous inhibitors. Another topic I am interested in and working on also is the Biopharming of Human pathogen virus as Virus Like Particles (VLPs) for candidate vaccine development in cereal grains (i.e. Hepatitis B surface antigen). Furthermore, one of my last topics of my research is metabolic engineering of carrot transcription factors (MYB/bHLH) involved in purple color of taproots by using overexpression techniques like cis-genesis and new knock-out methods like CRISPR/Cas9. The range of my collaboration includes Novozymes, Carlsberg, Chr. Hansen, Sejet Plant Breeding, Copenhagen University, University of East Anglia, Norwich UK, Leibniz Institut, Freising, Germany and others). New topics where I am specializing are: A) Bio-insecticides production based on recombinant entomopathogenic fungal enzymes and their in planta transgenic expression. B) Protease inhibitors biosynthesis by NonRibosomal Peptide Synthetases (NRPS) and recombinant based. C) Development of immunological based kits for plant pathogen detection. Giuseppe Dionisio is author of 24 peer-reviewed publications (h-index 11/Google Scholar), and active reviewer of many international peer-reviewed journals. Editor activities: Topic editor in Frontiers in Plant Biotechnology: “Biopharming: in plant versus microorganism recombinant bio-pharmaceuticals production” 

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